AB85916

Anti-Nup107抗体

Name: Nup107抗体 (ab85916)| Abcam中文官网
Brand: Abcam Limited
SKU: AB85916
Price: 4606 CNY
Availability: InStock

Anti-Nup107 antibody

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(2 Publications)

Rabbit Polyclonal Nup107 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human Nuclear pore complex protein Nup107 aa 850 to C-terminus.

查看别名

Nuclear pore complex protein Nup107, 107 kDa nucleoporin, Nucleoporin Nup107, NUP107

2 Images

Unknown

Immunoprecipitation - Anti-Nup107 antibody (AB85916)

Detection of Nup107 by Western Blot of Immunprecipitate.
ab85916 at 1µg/ml staining Nup107 in HeLa whole cell lysate immunoprecipitated using ab85916 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection : Chemiluminescence with exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-Nup107 antibody (ab85916)

Predicted band size: 106 kDa

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Unknown

Western blot - Anti-Nup107 antibody (AB85916)

All lanes:

Western blot - Anti-Nup107 antibody (ab85916) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Predicted band size: 106 kDa

Observed band size: 106 kDa

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Exposure time: 3min

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

IP, WB

applications

免疫原

Synthetic Peptide within Human Nuclear pore complex protein Nup107 aa 850 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P57740

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

纯化说明

ab85916 was affinity purified using an epitope specific to Nup107 immobilized on solid support.

存储溶液

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The Nup107 also known as nucleoporin 107 is an important protein component of the nuclear pore complex (NPC). With a molecular mass of approximately 107 kDa it is a significant element in the structure of the NPC. Nup107 is expressed in various tissues throughout the body. It plays an essential mechanical role in the assembly and maintenance of NPCs facilitating the transport of macromolecules between the nucleus and the cytoplasm.

Biological function summary

Nup107 forms part of the Nup107-160 subcomplex which contributes to the NPC's structural integrity and function. This complex acts as a scaffold providing support for the overall architecture and positioning within the nuclear envelope. The presence of Nup107 influences nuclear-cytoplasmic transport which is key in regulation of gene expression and cell cycle progression. Nup107 ensures proper assembly serving as an anchor for other nucleoporins within the NPC.

Pathways

The Nup107 subcomplex plays a critical role in key cellular processes such as the cell cycle and gene expression regulation. It participates in the mitotic entry and exit by regulating nucleocytoplasmic transport of cell cycle regulators and transcription factors. Nup107 interacts with proteins like Nup133 and Nup160 which assist in these processes. The precise functioning of Nup107 is necessary for maintaining cellular homeostasis and successful progression through cell division.

Nup107 has links to conditions such as primary ovarian insufficiency and nephrotic syndrome. Mutations or aberrant expression of Nup107 disrupt NPC assembly or function potentially leading to impaired cellular transport and altered cellular functions. In ovarian insufficiency altered Nup107 expression affects folliculogenesis by disrupting nuclear transport of specific factors. In nephrotic syndrome Nup107 dysfunction impacts the glomerular filtration barrier with associated proteins like nephrin and podocin demonstrating interrelated pathological effects.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Plays a role in the nuclear pore complex (NPC) assembly and/or maintenance (PubMed : 12552102, PubMed : 15229283, PubMed : 30179222). Required for the assembly of peripheral proteins into the NPC (PubMed : 12552102, PubMed : 15229283). May anchor NUP62 to the NPC (PubMed : 15229283). Involved in nephrogenesis (PubMed : 30179222).

See full target information Nuclear pore complex protein Nup107

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 5:3270 PubMed24509916

2014

The nucleoporin MEL-28 promotes RanGTP-dependent γ-tubulin recruitment and microtubule nucleation in mitotic spindle formation.

Applications

Unspecified application

Species

Unspecified reactive species

Hideki Yokoyama,Birgit Koch,Rudolf Walczak,Fulya Ciray-Duygu,Juan Carlos González-Sánchez,Damien P Devos,Iain W Mattaj,Oliver J Gruss

PLoS biology 11:e1001524 PubMed23555195

2013

A conserved role for human Nup98 in altering chromatin structure and promoting epigenetic transcriptional memory.

Applications

Unspecified application

Species

Unspecified reactive species

William H Light,Jonathan Freaney,Varun Sood,Abbey Thompson,Agustina D'Urso,Curt M Horvath,Jason H Brickner

View all publications

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Abcam Product Promise

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Anti-Nup107抗体

Anti-Nup107 antibody

Immunoprecipitation - Anti-Nup107 antibody (AB85916)

Western blot - Anti-Nup107 antibody (AB85916)

关键信息

宿主种属

克隆

亚型

不含载体蛋白

反应种属

应用

免疫原

反应性数据

性能和储存信息

形式

纯化工艺

纯化说明

存储溶液

运输条件

推荐的短期储存条件

推荐的长期储存条件

分装信息

储存信息

补充信息

Biological function summary

Pathways

产品实验方案

靶点信息

文献 (2)

Abcam Product Promise

Anti-Nup107抗体

Anti-Nup107 antibody

Immunoprecipitation - Anti-Nup107 antibody (AB85916)

Western blot - Anti-Nup107 antibody (AB85916)

关键信息

宿主种属

克隆

亚型

不含载体蛋白

反应种属

应用

免疫原

替代产品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

搭配使用产品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

反应性数据

性能和储存信息

形式

纯化工艺

纯化说明

存储溶液

运输条件

推荐的短期储存条件

推荐的长期储存条件

分装信息

储存信息

补充信息

Biological function summary

Pathways

产品实验方案

靶点信息

文献 (2)

Abcam Product Promise