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AB4147

Anti-NUMB 抗体

Anti-NUMB antibody

4

(10 Reviews)

|

(41 Publications)

Goat Polyclonal NUMB antibody. Suitable for Flow Cyt, IP, ELISA, WB, IHC-P, ICC/IF and reacts with Human, Mouse, Chicken samples. Cited in 41 publications. Immunogen corresponding to Synthetic Peptide within Human NUMB aa 600 to C-terminus.

查看别名

C14orf41, NUMB, Protein numb homolog, h-Numb, Protein S171

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUMB antibody (AB4147)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUMB antibody (AB4147)

Immunohistochemistry staining of paraffin embedded Human Prostate labeling NUMB with ab4147 at (8µg/mL). Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Sandwich ELISA - Anti-NUMB antibody (AB4147)
  • sELISA

Lab

Sandwich ELISA - Anti-NUMB antibody (AB4147)

Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (1µg/mL). IgG control : Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Western blot - Anti-NUMB antibody (AB4147)
  • WB

Supplier Data

Western blot - Anti-NUMB antibody (AB4147)

Detected by chemiluminescence.

All lanes:

Western blot - Anti-NUMB antibody (ab4147) at 1 µg/mL

All lanes:

Mouse brain lysates at 35 µg

Predicted band size: 71 kDa

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Flow Cytometry - Anti-NUMB antibody (AB4147)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-NUMB antibody (AB4147)

Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (1µg/mL). IgG control : Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-NUMB antibody (AB4147)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NUMB antibody (AB4147)

Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing plasma membrane and cytoplasmic staining. The nuclear stain is DAPI (blue).

Negative control : Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

Immunocytochemistry/ Immunofluorescence - Anti-NUMB antibody (AB4147)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NUMB antibody (AB4147)

Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cell junction and cytoplasmic staining. The nuclear stain is DAPI (blue).

Negative control : Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

Immunocytochemistry/ Immunofluorescence - Anti-NUMB antibody (AB4147)
  • ICC/IF

AbReview35128****

Immunocytochemistry/ Immunofluorescence - Anti-NUMB antibody (AB4147)

ab4147 staining NUMB in the NIH3T3 cell line from Mouse fibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 10% serum for 60 minutes at 24°C. Samples were incubated with primary antibody (1/100) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Donkey anti-rabbit polyclonal(1/500) was used as the secondary antibody.

This image is courtesy of an anonymous Abreview

关键信息

宿主种属

Goat

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Mouse, Human, Chicken

应用

IP, ELISA, IHC-P, WB, ICC/IF, Flow Cyt

applications

免疫原

Synthetic Peptide within Human NUMB aa 600 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P49757

特异性

This antibody is expected to recognize all reported isoforms (NP_001005743.1, 001005744.1, NP_003735, NP_001005745.1). It is also likely to cross-react with Numblike (NUMBL, GeneID: 9253) which has a very similar C-Terminus, although this has not yet been tested experimentally.

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反应性数据

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产品详情

This product may cross-react with Numblike (NUMBL, GeneID: 9253) Principal Names - NUMB; S171; numb homolog (Drosophila) Official Gene Symbol - NUMB GenBank Accession Number – NP_003735 LocusLink ID - 8650 (human); 18222 (mouse) Gene Ontology terms - integral to plasma membrane
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
存储溶液
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

NUMB also known as numb protein is a cytoplasmic protein involved in cell fate determination. It has an approximate molecular mass of 75 kDa. NUMB plays a critical role in asymmetric cell division particularly during neural development. The protein is expressed in various tissues including the brain heart and liver but most prominently in neural tissues. NUMB influences the timing of differentiation and proliferation by interacting with several cellular components.
Biological function summary

NUMB proteins are important to regulating cell fate decisions. NUMB primarily functions as a part of a larger complex including proteins like Notch and Eps15. In neural stem cells NUMB acts as an inhibitor of the Notch signaling pathway. It also regulates endocytosis and the signaling pathways that contribute to maintaining cellular diversity during development. NUMB interactions influence processes like cell adhesion and migration which are essential for proper tissue formation.

Pathways

NUMB proteins play significant roles within the Notch and Hedgehog signaling pathways. These are critical pathways for developmental processes. In the Notch pathway NUMB negatively regulates Notch receptor activity preventing Notch-mediated gene expression. NUMB interacts with other proteins such as alpha-adaptin and LNX1 through these pathways helping regulate the endocytic trafficking of key signaling molecules. In the Hedgehog pathway NUMB affects signal transduction though its role is less defined compared to its involvement in the Notch pathway.

NUMB proteins have been implicated in breast cancer and Alzheimer's disease. In breast cancer alterations in NUMB expression or function can contribute to tumor progression due to dysregulation of the Notch pathway. Its interaction with proteins like MDM2 influences the stability and activity of p53 an important tumor suppressor. In Alzheimer's disease NUMB's involvement in endocytic processes may impact amyloid precursor protein processing and beta-amyloid plaque formation which are central to the disease pathology.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Regulates clathrin-mediated receptor endocytosis (PubMed : 18657069). Plays a role in the process of neurogenesis (By similarity). Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate (By similarity). Not required for the proliferation of neural progenitor cells before the onset of neurogenesis. Also involved postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity (By similarity). May also mediate local repair of brain ventricular wall damage (By similarity).
See full target information NUMB
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文献 (41)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:2290 PubMed37085479

2023

RNA binding protein SYNCRIP maintains proteostasis and self-renewal of hematopoietic stem and progenitor cells.

Applications

Unspecified application

Species

Unspecified reactive species

Florisela Herrejon Chavez,Hanzhi Luo,Paolo Cifani,Alli Pine,Karen L Chu,Suhasini Joshi,Ersilia Barin,Alexandra Schurer,Mandy Chan,Kathryn Chang,Grace Y Q Han,Aspen J Pierson,Michael Xiao,Xuejing Yang,Lindsey M Kuehm,Yuning Hong,Diu T T Nguyen,Gabriela Chiosis,Alex Kentsis,Christina Leslie,Ly P Vu,Michael G Kharas

International journal of biological sciences 18:1896-1911 PubMed35342338

2022

MAD2B promotes podocyte injury through regulating Numb-dependent Notch 1 pathway in diabetic nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Meng-Ran Li,Chun-Tao Lei,Hui Tang,Xing-Jie Yin,Zhe Hao,Yang Qiu,Ya-Ru Xie,Jie-Yu Zeng,Hua Su,Chun Zhang

eLife 10: PubMed33899739

2021

DIAPH3 deficiency links microtubules to mitotic errors, defective neurogenesis, and brain dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Eva On-Chai Lau,Devid Damiani,Georges Chehade,Nuria Ruiz-Reig,Rana Saade,Yves Jossin,Mohamed Aittaleb,Olivier Schakman,Nicolas Tajeddine,Philippe Gailly,Fadel Tissir

Nature communications 12:1065 PubMed33594067

2021

LIGHT/LTβR signaling regulates self-renewal and differentiation of hematopoietic and leukemia stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

S S Höpner,Ana Raykova,R Radpour,M A Amrein,D Koller,G M Baerlocher,C Riether,A F Ochsenbein

Aging cell 20:e13310 PubMed33524216

2021

Decreased p53 is associated with a decline in asymmetric stem cell self-renewal in aged human epidermis.

Applications

Unspecified application

Species

Unspecified reactive species

Alexandra Charruyer,Tracy Weisenberger,Hang Li,Ayman Khalifa,Andrew W Schroeder,Annika Belzer,Ruby Ghadially

Molecular brain 14:20 PubMed33478555

2021

Non-SUMOylated CRMP2 decreases Na1.7 currents via the endocytic proteins Numb, Nedd4-2 and Eps15.

Applications

Unspecified application

Species

Unspecified reactive species

Kimberly Gomez,Dongzhi Ran,Cynthia L Madura,Aubin Moutal,Rajesh Khanna

Cell reports 30:3605-3615.e5 PubMed32187533

2020

A Model of Differential Mammary Growth Initiation by Stat3 and Asymmetric Integrin-α6 Inheritance.

Applications

Unspecified application

Species

Unspecified reactive species

Edward J Morris,Jordan A Gillespie,Christopher A Maxwell,Shoukat Dedhar

American journal of physiology. Gastrointestinal a 318:G841-G853 PubMed32146835

2020

negatively regulates the epithelial-to-mesenchymal transition in colorectal cancer through the Wnt signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Chi Cheng,Zhenfeng Huang,Ruiyao Zhou,Huimin An,Gaojian Cao,Jun Ye,Chaolin Huang,Daoyi Wu

GeroScience 42:595-611 PubMed32088828

2020

CD82-TRPM7-Numb signaling mediates age-related cognitive impairment.

Applications

Unspecified application

Species

Unspecified reactive species

Yin Zhao,Tamas Kiss,Jordan DelFavero,Lu Li,Xing Li,Lu Zheng,Jie Wang,Chao Jiang,Jing Shi,Zoltan Ungvari,Anna Csiszar,Xin A Zhang

Oncogenesis 9:20 PubMed32054830

2020

CUL4B contributes to cancer stemness by repressing tumor suppressor miR34a in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yanjun Li,Huili Hu,Yuxing Wang,Yujia Fan,Yang Yang,Beibei Guo,Xueyong Xie,Jiabei Lian,Baichun Jiang,Bo Han,Yanlei Wang,Changshun Shao,Yaoqin Gong
View all publications
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