AB264224

Anti-NUMB抗体

Name: NUMB抗体 (ab264224)| Abcam中文官网
Brand: Abcam Limited
SKU: AB264224
Price: 4863 CNY
Availability: InStock

Anti-NUMB antibody

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(1 Publication)

Rabbit Polyclonal NUMB antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human NUMB aa 350-450.

查看别名

C14orf41, NUMB, Protein numb homolog, h-Numb, Protein S171

2 Images

Supplier Data

Immunoprecipitation - Anti-NUMB antibody (AB264224)

NUMB was immunoprecipitated from 1 mg HeLa whole cell lysate using ab264224 at 3 μg/mg lysate. Western blot was performed on the immunoprecipitate using ab264224 at 0.1 μg/ml.

Lane 1 : ab264224 IP in HeLa whole cell lysate.
Lane 2 : Control IgG.

All lanes:

Immunoprecipitation - Anti-NUMB antibody (ab264224)

Predicted band size: 71 kDa

true

Exposure time: 30s

Supplier Data

Western blot - Anti-NUMB antibody (AB264224)

All lanes:

Western blot - Anti-NUMB antibody (ab264224) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

HEK-293T whole cell lysate at 50 µg

Predicted band size: 71 kDa

true

Exposure time: 30s

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

WB, IP

applications

免疫原

Synthetic Peptide within Human NUMB aa 350-450. The exact immunogen used to generate this antibody is proprietary information.

P49757

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

NUMB also known as numb protein is a cytoplasmic protein involved in cell fate determination. It has an approximate molecular mass of 75 kDa. NUMB plays a critical role in asymmetric cell division particularly during neural development. The protein is expressed in various tissues including the brain heart and liver but most prominently in neural tissues. NUMB influences the timing of differentiation and proliferation by interacting with several cellular components.

Biological function summary

NUMB proteins are important to regulating cell fate decisions. NUMB primarily functions as a part of a larger complex including proteins like Notch and Eps15. In neural stem cells NUMB acts as an inhibitor of the Notch signaling pathway. It also regulates endocytosis and the signaling pathways that contribute to maintaining cellular diversity during development. NUMB interactions influence processes like cell adhesion and migration which are essential for proper tissue formation.

Pathways

NUMB proteins play significant roles within the Notch and Hedgehog signaling pathways. These are critical pathways for developmental processes. In the Notch pathway NUMB negatively regulates Notch receptor activity preventing Notch-mediated gene expression. NUMB interacts with other proteins such as alpha-adaptin and LNX1 through these pathways helping regulate the endocytic trafficking of key signaling molecules. In the Hedgehog pathway NUMB affects signal transduction though its role is less defined compared to its involvement in the Notch pathway.

NUMB proteins have been implicated in breast cancer and Alzheimer's disease. In breast cancer alterations in NUMB expression or function can contribute to tumor progression due to dysregulation of the Notch pathway. Its interaction with proteins like MDM2 influences the stability and activity of p53 an important tumor suppressor. In Alzheimer's disease NUMB's involvement in endocytic processes may impact amyloid precursor protein processing and beta-amyloid plaque formation which are central to the disease pathology.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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靶点信息

Regulates clathrin-mediated receptor endocytosis (PubMed : 18657069). Plays a role in the process of neurogenesis (By similarity). Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate (By similarity). Not required for the proliferation of neural progenitor cells before the onset of neurogenesis. Also involved postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity (By similarity). May also mediate local repair of brain ventricular wall damage (By similarity).

See full target information NUMB

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