重组Anti-NUDT5抗体[EPR7735] (ab129163)
![Western blot - Anti-NUDT5 antibody [EPR7735] (ab129163)](https://www.abcam.cn/ps/products/129/ab129163/Images/ab129163-397643-anti-nudt5-antibody-epr7735-western-blot-human-knockout-lysate.jpg "Western blot - Anti-NUDT5 antibody [EPR7735] (ab129163)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7735] to NUDT5
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-NUDT5抗体[EPR7735]
参阅全部 NUDT5 一抗 -
描述
兔单克隆抗体[EPR7735] to NUDT5 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF or IHC-P -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human NUDT5 aa 150-250. The exact sequence is proprietary.
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阳性对照
- WB: HeLa, 293T, HepG2 and U937 cell lysates
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数（KD）
KD = 3.48 x 10 -10 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR7735 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab129163于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 33 kDa (predicted molecular weight: 24 kDa).
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| 说明 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 33 kDa (predicted molecular weight: 24 kDa). |
靶标
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功能
Hydrolyzes with similar activities ADP-ribose, ADP-mannose, and ADP-glucose. Can also hydrolyze other nucleotide sugars with low activity. -
组织特异性
Widely expressed. Most abundant in liver. -
序列相似性
Belongs to the Nudix hydrolase family.
Contains 1 nudix hydrolase domain. - Information by UniProt
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数据库链接
- Entrez Gene: 11164 Human
- Omim: 609230 Human
- SwissProt: Q9UKK9 Human
- Unigene: 555956 Human
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别名
- ADP sugar pyrophosphatase antibody
- ADP-sugar pyrophosphatase antibody
- hYSAH 1 antibody
see all
图片
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Western blot - Anti-NUDT5 antibody [EPR7735] (ab129163)All lanes : Anti-NUDT5 antibody [EPR7735] (ab129163) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : NUDT5 knockout HEK293T cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 24 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab129163 observed at 30 kDa. Red - loading control ab8245 observed at 36 kDa.
ab129163 Anti-NUDT5 antibody [EPR7735] was shown to specifically react with NUDT5 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266242 (knockout cell lysate ab258087) was used. Wild-type and NUDT5 knockout samples were subjected to SDS-PAGE. ab129163 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-NUDT5 antibody [EPR7735] (ab129163)](https://www.abcam.cn/ps/products/129/ab129163/Images/ab129163-143163-anti-nudt5-antibody-epr7735-western-blot.jpg)
Western blot - Anti-NUDT5 antibody [EPR7735] (ab129163)All lanes : Anti-NUDT5 antibody [EPR7735] (ab129163) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : U937 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution
Predicted band size: 24 kDa
Observed band size: 33 kDa why is the actual band size different from the predicted? -
![OI-RD Scanning - Anti-NUDT5 antibody [EPR7735] (ab129163)](https://www.abcam.cn/ps/products/129/ab129163/Images/ab129163-143162-anti-nudt5-antibody-epr7735.jpg)
OI-RD Scanning - Anti-NUDT5 antibody [EPR7735] (ab129163) -
![Anti-NUDT5 antibody [EPR7735] (ab129163)](https://www.abcam.cn/ps/products/129/ab129163/Images/ab129163-4-benefits-of-recombinant-antibodies.png)
Anti-NUDT5 antibody [EPR7735] (ab129163)
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab129163 尚未被引用在任何文献中。