重组Anti-Nucleostemin抗体[EPR3911(2)] (ab129185)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3911(2)] to Nucleostemin
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
概述
-
产品名称
Anti-Nucleostemin抗体[EPR3911(2)]
参阅全部 Nucleostemin 一抗 -
描述
兔单克隆抗体[EPR3911(2)] to Nucleostemin -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WBmore details
不适用于: IHC-P or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- HeLa, PC-3, SW480, and K562 cell lysates, Permeabilized SW480 cells
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
解离常数(KD)
KD = 6.76 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
-
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR3911(2) -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Isotype control
-
Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab129185于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/1000 - 1/10000. Predicted molecular weight: 62 kDa.
|
说明 |
---|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Predicted molecular weight: 62 kDa. |
靶标
-
功能
May be required to maintain the proliferative capacity of stem cells and may play an important role in tumorigenesis. -
组织特异性
Increased levels in lung tissue in cancer patients. -
序列相似性
Belongs to the MMR1/HSR1 GTP-binding protein family.
Contains 1 G (guanine nucleotide-binding) domain. -
结构域
The basic domain (B) allows nucleolar localization in the absence of GTP. The intermediate domain (I) inhibits nucleolar localization by the B domain and is required for exit from the nucleolus. Exit from the nucleolus to the nucleoplasm requires both the I and the acidic (A) domains, and may be triggered by GTP hydrolysis.
In contrast to other GTP-binding proteins, this family is characterized by a circular permutation of the GTPase motifs described by a G4-G1-G3 pattern. -
细胞定位
Nucleus. Nucleus > nucleolus. Shuttles between the nucleus and nucleolus. - Information by UniProt
-
数据库链接
- Entrez Gene: 26354 Human
- Omim: 608011 Human
- SwissProt: Q9BVP2 Human
- Unigene: 313544 Human
-
别名
- C77032 antibody
- E2 induced gene 3 protein antibody
- E2-induced gene 3 protein antibody
see all
图片
-
All lanes : Anti-Nucleostemin antibody [EPR3911(2)] (ab129185) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : PC-3 cell lysate
Lane 3 : SW480 cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 62 kDa -
Intracellular flow cytometric analysis of permeabilized SW480 cells using ab129185 (red) at 1/10 dilution or a rabbit IgG (negative) (green).
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab129185 尚未被引用在任何文献中。