Anti-Nucleostemin抗体(ab70346)
Key features and details
- Rabbit polyclonal to Nucleostemin
- Suitable for: ICC/IF, IHC-P, WB, IP
- Reacts with: Mouse, Rat, Chicken, Human
- Isotype: IgG
概述
-
产品名称
Anti-Nucleostemin抗体
参阅全部 Nucleostemin 一抗 -
描述
兔多克隆抗体to Nucleostemin -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, IHC-P, WB, IPmore details -
种属反应性
与反应: Mouse, Rat, Chicken, Human
预测可用于: Rabbit, Horse, Dog, Chimpanzee
-
免疫原
Synthetic peptide corresponding to Human Nucleostemin (C terminal).
Database link: Q9BVP2 -
阳性对照
- HeLa, 293T and mouse 3T3 whole cell lysates.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab70346 was affinity purified using an epitope specific to Nucleostemin immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab70346于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (2) |
Use a concentration of 1 µg/ml.
|
| IHC-P | (3) |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| WB | (4) |
1/2000 - 1/10000. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
|
| IP | (1) |
Use at 1-2 µg/mg of lysate.
|
| 说明 |
|---|
|
ICC/IF
Use a concentration of 1 µg/ml. |
|
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
1/2000 - 1/10000. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa). |
|
IP
Use at 1-2 µg/mg of lysate. |
靶标
-
功能
May be required to maintain the proliferative capacity of stem cells and may play an important role in tumorigenesis. -
组织特异性
Increased levels in lung tissue in cancer patients. -
序列相似性
Belongs to the MMR1/HSR1 GTP-binding protein family.
Contains 1 G (guanine nucleotide-binding) domain. -
结构域
The basic domain (B) allows nucleolar localization in the absence of GTP. The intermediate domain (I) inhibits nucleolar localization by the B domain and is required for exit from the nucleolus. Exit from the nucleolus to the nucleoplasm requires both the I and the acidic (A) domains, and may be triggered by GTP hydrolysis.
In contrast to other GTP-binding proteins, this family is characterized by a circular permutation of the GTPase motifs described by a G4-G1-G3 pattern. -
细胞定位
Nucleus. Nucleus > nucleolus. Shuttles between the nucleus and nucleolus. - Information by UniProt
-
数据库链接
- Entrez Gene: 26354 Human
- Entrez Gene: 30877 Mouse
- Entrez Gene: 290556 Rat
- Omim: 608011 Human
- SwissProt: Q9BVP2 Human
- SwissProt: Q8CI11 Mouse
- SwissProt: Q811S9 Rat
- Unigene: 313544 Human
see all -
别名
- C77032 antibody
- E2 induced gene 3 protein antibody
- E2-induced gene 3 protein antibody
see all
图片
-
Western blot - Anti-Nucleostemin antibody (ab70346)All lanes : Anti-Nucleostemin antibody (ab70346) at 1/5000 dilution
Lane 1 : HeLa Whole cell lysate
Lane 2 : HEK293T Whole cell lysate
Lane 3 : Jurkat Whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Goat anti-Rabbit Light Chain HRP Conjugate
Predicted band size: 62 kDaDetection: Chemiluminescence with an exposure time of 10 seconds
-
Western blot - Anti-Nucleostemin antibody (ab70346)All lanes : Anti-Nucleostemin antibody (ab70346) at 1/2000 dilution
Lane 1 : TMCK-1 Whole cell lysate
Lane 2 : NIH 3T3 :Whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Goat anti-Rabbit Light Chain HRP Conjugate
Predicted band size: 62 kDaDetection: Chemiluminescence with an exposure time of 30 seconds.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleostemin antibody (ab70346)Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded section of mouse CT26 colon carcinoma tissue labelling Nucleostemin with ab70346 at 1:200 (1µg/ml). Detection: DAB.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleostemin antibody (ab70346)Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded section of human breast carcinoma tissue labelling Nucleostemin with ab70346 at 1:200 (1µg/ml). Detection: DAB.
-
Immunoprecipitation - Anti-Nucleostemin antibody (ab70346)Nucleostemin was immunoprecipitated from HEK-293T whole cell lysate with 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab70346 at 0.04 µg/ml dilution.
Lane 1: ab70346 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
-
Immunocytochemistry/ Immunofluorescence - Anti-Nucleostemin antibody (ab70346)ICC/IF image of ab70346 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70346, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleostemin antibody (ab70346)This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United KingdomImmunohistochemistical detection of Nucleostemin with ab70346 on formaldehyde-fixed paraffin-embedded human colon sections. Antigen retrieval step: heat mediated in citric acid pH6 buffer. Blocking step: 1% BSA for 10 mins @ rt°C. Primary antibody ab70346 incubated at 1/50 for 16 hours in TBS/BSA/azide. Secondary antibody: anti-rabbit IgG conjugated to biotin (1/200). The intestinal epithelium is the most rapidly self-renewing tissue in adult mammals. Stem cell markers have been observed in cycling columnar cells at the crypt base (Nature 449, 1003-1007; 2007). The cells (nuclei of the simple columnar epithelium of the upper mucosa) immunoreactive for Nucleostemin in this image (green arrowheads) anatomically correspond to the same cells expected to be Lgr5 positivite. A red arrowhead indicates nucleolar positivity in one of many cell nuclei in the Lamina propria of the mucosa (this area is not known for stem cell posit
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (16)
ab70346 被引用在 16 文献中.
- Fujiwara-Tani R et al. Short Telomere Lesions with Dysplastic Metaplasia Histology May Represent Precancerous Lesions of Helicobacter pylori-Positive Gastric Mucosa. Int J Mol Sci 24:N/A (2023). PubMed: 36834592
- Gyoten M et al. Lovastatin Treatment Inducing Apoptosis in Human Pancreatic Cancer Cells by Inhibiting Cholesterol Rafts in Plasma Membrane and Mitochondria. Int J Mol Sci 24:N/A (2023). PubMed: 38069135
- Kondo Y et al. Endosialin/CD248 may be a potential therapeutic target to prevent the invasion and metastasis in osteosarcoma. Oncol Lett 23:42 (2022). PubMed: 34976154
- Sukocheva OA et al. Divergence of Intracellular Trafficking of Sphingosine Kinase 1 and Sphingosine-1-Phosphate Receptor 3 in MCF-7 Breast Cancer Cells and MCF-7-Derived Stem Cell-Enriched Mammospheres. Int J Mol Sci 22:N/A (2021). PubMed: 33919234
- Fujiwara-Tani R et al. BRAF Mutation Is Associated with Hyperplastic Polyp-Associated Gastric Cancer. Int J Mol Sci 22:N/A (2021). PubMed: 34884530