重组Anti-Nucleoside phosphorylase抗体[EPR5715] (ab109447)
![Western blot - Anti-Nucleoside phosphorylase antibody [EPR5715] (ab109447)](https://www.abcam.cn/ps/products/109/ab109447/Images/ab109447-393576-anti-nucleoside-phosphorylase-antibody-epr5715-western-blot-human-knockout-lysate.jpg "Western blot - Anti-Nucleoside phosphorylase antibody [EPR5715] (ab109447)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5715] to Nucleoside phosphorylase
- Suitable for: WB, ICC/IF
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Nucleoside phosphorylase抗体[EPR5715]
参阅全部 Nucleoside phosphorylase 一抗 -
描述
兔单克隆抗体[EPR5715] to Nucleoside phosphorylase -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details
不适用于: IHC-P or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- HeLa, Jurkat, JAR, K562, Human placenta, and 293T lysates. ICC/IF: SH SY5Y cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数（KD）
KD = 1.76 x 10 -10 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5715 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109447于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 31 kDa (predicted molecular weight: 32 kDa).
|
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| ICC/IF |
1/100 - 1/250.
|
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 31 kDa (predicted molecular weight: 32 kDa). |
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ICC/IF
1/100 - 1/250. |
靶标
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疾病相关
Defects in PNP are the cause of purine nucleoside phosphorylase deficiency (PNP deficiency) [MIM:613179]. It leads to a severe T-cell immunodeficiency with neurologic disorder in children. -
序列相似性
Belongs to the PNP/MTAP phosphorylase family. -
细胞定位
Cytoplasm > cytoskeleton. - Information by UniProt
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数据库链接
- Entrez Gene: 4860 Human
- Omim: 164050 Human
- SwissProt: P00491 Human
- Unigene: 75514 Human
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别名
- FLJ94043 antibody
- FLJ97288 antibody
- FLJ97312 antibody
see all
图片
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Western blot - Anti-Nucleoside phosphorylase antibody [EPR5715] (ab109447)All lanes : Anti-Nucleoside phosphorylase antibody [EPR5715] (ab109447) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : PNP knockout HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 32 kDa
Observed band size: 31 kDa why is the actual band size different from the predicted?Lanes 1-3: Merged signal (red and green). Green - ab109447 observed at 31 kDa. Red - loading control ab7291 observed at 50 kDa.
ab109447 Anti-Nucleoside phosphorylase antibody [EPR5715] was shown to specifically react with Nucleoside phosphorylase in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab266158 (knockout cell lysate ab257594) was used. Wild-type and Nucleoside phosphorylase knockout samples were subjected to SDS-PAGE. ab109447 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunocytochemistry/ Immunofluorescence - Anti-Nucleoside phosphorylase antibody [EPR5715] (ab109447)](https://www.abcam.cn/ps/products/109/ab109447/Images/ab109447-122311-anti-nucleoside-phosphorylase-antibody-epr5715-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Nucleoside phosphorylase antibody [EPR5715] (ab109447)ab109447 at 1/100 dilution staining Nucleoside phosphorylase in SH SY5Y cells by Immunofluorescence. -
![Western blot - Anti-Nucleoside phosphorylase antibody [EPR5715] (ab109447)](https://www.abcam.cn/ps/products/109/ab109447/Images/ab109447-122312-anti-nucleoside-phosphorylase-antibody-epr5715-western-blot.jpg)
Western blot - Anti-Nucleoside phosphorylase antibody [EPR5715] (ab109447)All lanes : Anti-Nucleoside phosphorylase antibody [EPR5715] (ab109447) at 1/1000 dilution
Lane 1 : Jurkat lysate
Lane 2 : JAR lysate
Lane 3 : K562 lysate
Lane 4 : Human placenta lysate
Lane 5 : 293T lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 32 kDa
Observed band size: 31 kDa why is the actual band size different from the predicted? -
![OI-RD Scanning - Anti-Nucleoside phosphorylase antibody [EPR5715] (ab109447)](https://www.abcam.cn/ps/products/109/ab109447/Images/ab109447-122310-anti-nucleoside-phosphorylase-antibody-epr5715.jpg)
OI-RD Scanning - Anti-Nucleoside phosphorylase antibody [EPR5715] (ab109447) -
![Anti-Nucleoside phosphorylase antibody [EPR5715] (ab109447)](https://www.abcam.cn/ps/products/109/ab109447/Images/ab109447-5-benefits-of-recombinant-antibodies.png)
Anti-Nucleoside phosphorylase antibody [EPR5715] (ab109447)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab109447 尚未被引用在任何文献中。