AB212671

Anti-Nucleolin抗体[SPM614] - BSA and Azide free

Name: Nucleolin抗体[SPM614] - BSA and Azide free (ab212671)| Abcam中文官网
Brand: Abcam Limited
SKU: AB212671
Price: 9609 CNY
Availability: InStock

Anti-Nucleolin antibody [SPM614] - BSA and Azide free

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(1 Publication)

Mouse Monoclonal Nucleolin antibody. Carrier free. Suitable for IHC-P, ICC and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human NCL.

查看别名

Nucleolin, Protein C23, NCL

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin antibody [SPM614] - BSA and Azide free (AB212671)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin antibody [SPM614] - BSA and Azide free (AB212671)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue section labeling Nucleolin with ab212671 at 1 μg/mL.

Supplier Data

Immunocytochemistry - Anti-Nucleolin antibody [SPM614] - BSA and Azide free (AB212671)

Immunocytochemistry analysis of K562 (Human chronic myelogenous leukemia lymphoblast cell line) cells labeling Nucleolin with ab212671 at 1 ug/ml follower by goat anti-mouse IgG-CF488 (green). Membrane stained with phalloidin-CF640 (red)

Supplier Data

Immunocytochemistry - Anti-Nucleolin antibody [SPM614] - BSA and Azide free (AB212671)

Immunocytochemistry analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Nucleolin with ab212671 at 1 ug/ml (green). Nuclei stained with DAPI (blue).

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

SPM614

亚型

IgG1

轻链类型

kappa

不含载体蛋白

Yes

反应种属

Human

应用

IHC-P, ICC

applications

免疫原

Recombinant Full Length Protein corresponding to Human NCL.

P19338

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.5-1 µg/mL", "IHCP-species-notes": "for 30 minutes at room temperature. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1-4 µg/mL", "ICC-species-notes": "" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "0.5-1 µg/mL", "IHCP-species-notes": "for 30 minutes at room temperature. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "0.5-1 µg/mL", "ICC-species-notes": "" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "0.5-1 µg/mL", "IHCP-species-notes": "for 30 minutes at room temperature. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "1-4 µg/mL", "ICC-species-notes": "" }, "Cow": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "0.5-1 µg/mL", "IHCP-species-notes": "for 30 minutes at room temperature. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "1-4 µg/mL", "ICC-species-notes": "" } } }

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产品详情

ab212671 can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein G

纯化说明

Purified from bioreactor concentrate.

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Nucleolin also known as C23 is a multifunctional protein mainly found in the nucleolus with a molecular weight of approximately 100 kDa. It plays a mechanical role in ribosomal RNA (rRNA) synthesis and ribosome biogenesis. Nucleolin is widely expressed in proliferating cells including HeLa culture and shows higher expression in cancer cells compared to normal cells. The nucleolin protein is involved in various nucleolar processes like the modulation of chromatin structure and nucleocytoplasmic transport.

Biological function summary

The nucleolin molecular weight protein contributes to regulating cellular processes such as chromatin remodeling rRNA transcription and mRNA stability. Nucleolin often forms complexes with various other proteins and nucleic acids enhancing its functionality within the cell nucleus. Its participation in processes like DNA replication and cell proliferation shows the central role nucleolin plays at the cellular level. It influences cellular responses by binding directly to specific RNA or DNA sequences.

Pathways

The nucleolin protein engages in major cellular pathways such as the RNA processing and p53 signaling pathways. Through the RNA processing pathway it interacts with proteins like fibrillarin to assist in rRNA maturation. Moreover within the p53 signaling pathway nucleolin regulates the activity of the tumor suppressor protein p53 impacting cell cycle progression and apoptosis. These interactions highlight nucleolin's role in maintaining cellular homeostasis and response to stress.

The nucleolin protein often shows altered expression in cancers and cardiovascular diseases. In cancer nucleolin's high expression levels correlate with tumor progression and metastasis making it a potential target for cancer therapies. In cardiovascular diseases nucleolin interacts with proteins like VEGF to influence angiogenesis which affects disease progression. Understanding nucleolin's involvement in these diseases provides insight into potential therapeutic targets and interventions.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.

See full target information NCL

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Biomedicines 11: PubMed37760875

2023

Creation of an iPSC-Based Skeletal Muscle Model of McArdle Disease Harbouring the Mutation c.2392T>C (p.Trp798Arg) in the Gene.

Applications

Unspecified application

Species

Unspecified reactive species

Victoria Cerrada,Inés García-Consuegra,Joaquín Arenas,M Esther Gallardo

View all publications

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Anti-Nucleolin抗体[SPM614] - BSA and Azide free

Anti-Nucleolin antibody [SPM614] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin antibody [SPM614] - BSA and Azide free (AB212671)

Immunocytochemistry - Anti-Nucleolin antibody [SPM614] - BSA and Azide free (AB212671)

Immunocytochemistry - Anti-Nucleolin antibody [SPM614] - BSA and Azide free (AB212671)

关键信息

宿主种属

克隆

克隆号

亚型

轻链类型

不含载体蛋白

反应种属

应用

免疫原