Anti-Nucleolin抗体(ab70493)
Key features and details
- Rabbit polyclonal to Nucleolin
- Suitable for: IHC-P, ICC/IF, ICC, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
概述
-
产品名称
Anti-Nucleolin抗体
参阅全部 Nucleolin 一抗 -
描述
兔多克隆抗体to Nucleolin -
宿主
Rabbit -
经测试应用
适用于: IHC-P, ICC/IF, ICC, WB, IPmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, African green monkey, Orangutan, Elephant
-
免疫原
Synthetic peptide within Human Nucleolin aa 550 to the C-terminus (C terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P19338 -
阳性对照
- WB: HeLa, HEK-293T and NIH/3T3 whole cell lysate. IHC-P: Human ovarian carcinoma and mouse squamous cell carcinoma tissue. Human normal lymph node tissue. Mouse cerebellum tissue. IP: HeLa whole cell lysate. ICC/IF: HeLa cells.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab70493于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| ICC/IF | (1) |
Use at an assay dependent concentration.
|
| ICC |
Use a concentration of 5 µg/ml.
|
|
| WB | (2) |
1/2000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 77 kDa).
|
| IP |
Use at 2-5 µg/mg of lysate.
|
| 说明 |
|---|
|
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ICC/IF
Use at an assay dependent concentration. |
|
ICC
Use a concentration of 5 µg/ml. |
|
WB
1/2000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 77 kDa). |
|
IP
Use at 2-5 µg/mg of lysate. |
靶标
-
功能
Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats. -
序列相似性
Contains 4 RRM (RNA recognition motif) domains. -
翻译后修饰
Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group. -
细胞定位
Nucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. - Information by UniProt
-
数据库链接
- Entrez Gene: 4691 Human
- Entrez Gene: 17975 Mouse
- Entrez Gene: 100174230 Orangutan
- Omim: 164035 Human
- SwissProt: P19338 Human
- SwissProt: P09405 Mouse
- SwissProt: Q5RF26 Orangutan
- Unigene: 79110 Human
see all -
别名
- C23 antibody
- FLJ45706 antibody
- MS1116 antibody
see all
图片
-
Western blot - Anti-Nucleolin antibody (ab70493)All lanes : Anti-Nucleolin antibody (ab70493) at 0.02 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Lane 5 : Whole cell lysate from NIH3T3 cells at 50 µg
Predicted band size: 77 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin antibody (ab70493)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma (left) and mouse squamous cell carcinoma (right) tissues labelling Nucleolin with ab70493 at 1/1000 (0.2µg/ml). Detection: DAB. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin antibody (ab70493)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebellum tissue labelling Nucleolin with ab70493 at 1/100 (2 µg/ml), followed by goat anti-rabbit IgG-heavy and light chain cross-adsorbed antibody DyLight® 594 used at 1/100 dilution.
-
Immunoprecipitation - Anti-Nucleolin antibody (ab70493)Detection of Human Nucleolin by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab70493 at 3 µg/mg lysate (Lane 1). Lane 2 represents rabbit IgG IP control. Subsequent Western blot detection of nucleolin was performed using ab70493 at 1 µg/ml. -
Immunocytochemistry/ Immunofluorescence - Anti-Nucleolin antibody (ab70493)ICC/IF image of ab70493 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70493, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin antibody (ab70493)IHC image of ab70493 staining in human normal lymph node formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70493, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (17)
ab70493 被引用在 17 文献中.
- Potapova TA et al. Distinct states of nucleolar stress induced by anticancer drugs. Elife 12:N/A (2023). PubMed: 38099650
- Moudry P et al. RNA-interference screen for p53 regulators unveils a role of WDR75 in ribosome biogenesis. Cell Death Differ 29:687-696 (2022). PubMed: 34611297
- Mirra S et al. Ubiquitin Specific Protease USP48 Destabilizes NF-κB/p65 in Retinal Pigment Epithelium Cells. Int J Mol Sci 23:N/A (2022). PubMed: 36077078
- Angrand G et al. Type I arginine methyltransferases are intervention points to unveil the oncogenic Epstein-Barr virus to the immune system. Nucleic Acids Res 50:11799-11819 (2022). PubMed: 36350639
- Goldson TM et al. Nucleolin mediates the binding of cancer cells to L-selectin under conditions of lymphodynamic shear stress. Am J Physiol Cell Physiol 318:C83-C93 (2020). PubMed: 31644306