AB136649

Anti-Nucleolin抗体[364-5]

Name: Nucleolin抗体[364-5] (ab136649)| Abcam中文官网
Brand: Abcam Limited
SKU: AB136649
Price: 4844 CNY
Availability: InStock
Rating: 5 (2 reviews)

Anti-Nucleolin antibody [364-5]

BOND RX™ Validated
了解详情

(2 Reviews)

(18 Publications)

Anti-Nucleolin antibody [364-5] (ab136649) is a mouse monoclonal antibody detecting Nucleolin in Western Blot, Flow Cytometry, IP, IHC-P, ICC/IF. Suitable for Human.

- Over 10 publications

查看别名

Nucleolin, Protein C23, NCL

7 Images

Immunocytochemistry/ Immunofluorescence - Anti-Nucleolin antibody [364-5] (AB136649)

ICC/IF

AbReview41541****

Immunocytochemistry/ Immunofluorescence - Anti-Nucleolin antibody [364-5] (AB136649)

ab136649 staining Nucleolin in human breast cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 5% Triton X-100 in PBS and blocked with 5% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/1000) for 1 hour. An undiluted Alexa Fluor® 546-conjugated anti-mouse IgG polyclonal was used as the secondary antibody.

This image is courtesy of an anonymous Abreview

Flow Cytometry - Anti-Nucleolin antibody [364-5] (AB136649)

Flow Cyt

Unknown

Flow Cytometry - Anti-Nucleolin antibody [364-5] (AB136649)

Flow Cytometric analysis of HeLa cells (fixed and permeabilized with methanol) labelling Nucleolin with ab136649 (blue) at 1µg/ml or an equal amount of an isotype control antibody (red).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin antibody [364-5] (AB136649)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin antibody [364-5] (AB136649)

IHC image of nucleolin staining in human liver HCC formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab136649, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Nucleolin antibody [364-5] (AB136649)

Immunocytochemical analysis of HDFn cells (paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15 min)) labelling Nucleolin with ab136649 at 1µg/ml (red) and anti-GAPDH (ab110305) in green, as a counter stain. Secondary : Alexa 594 goat anti-mouse IgG1 at a 1/1000 dilution.

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Nucleolin antibody [364-5] (AB136649)

Immunocytochemical analysis of HeLa cells (paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15 min)) labelling Nucleolin with ab136649 at 1μg/ml (red) and anti-GAPDH (ab110305) in green, as a counter stain.
Secondary : Alexa 594 goat anti-mouse IgG1 at a 1/1000 dilution.

Unknown

Immunoprecipitation - Anti-Nucleolin antibody [364-5] (AB136649)

Detection of Nucleolin by Immunoprecipitation.
Nucleolin in HepG2 cell lysate (1 mg) was immunoprecipitated using 1 µg ab136649 antibody. The gel was stained with Coomassie brilliant blue G.

All lanes:

Immunoprecipitation - Anti-Nucleolin antibody [364-5] (ab136649)

Predicted band size: 76 kDa

false

Unknown

Western blot - Anti-Nucleolin antibody [364-5] (AB136649)

All lanes:

Western blot - Anti-Nucleolin antibody [364-5] (ab136649) at 1 µg/mL

Lane 1:

HepG2 whole cell lysate at 20 µg

Lane 2:

HDFn whole cell lysate at 25 µg

Lane 3:

SH-SY5Y whole cell lysate at 20 µg

Secondary

All lanes:

Goat polyclonal to Mouse IgG - AP at 1/3000 dilution

Predicted band size: 76 kDa

false

不同偶联物与剂型 (2)

519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Nucleolin antibody [364-5]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Nucleolin antibody [364-5]

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

364-5

亚型

IgG1

不含载体蛋白

反应种属

Human

应用

ICC/IF, Flow Cyt, IHC-P, IP, WB

applications

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1 µg/mL", "IP-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg/mL", "FlowCyt-species-notes": "<a href='/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1 µg/mL", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1 µg/mL", "FlowCyt-species-notes": "<a href='/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1 µg/mL", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1 µg/mL", "FlowCyt-species-notes": "<a href='/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1 µg/mL", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1 µg/mL", "FlowCyt-species-notes": "<a href='/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "" } } }

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产品详情

What is this antibody validated in?
Anti-Nucleolin antibody [364-5] (ab136649) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

What is the molecular weight of Nucleolin?
Anti-Nucleolin [364-5] (ab136649) specifically detects a band for Nucleolin (UniProt: P19338) at a molecular weight of 76kDa.

Trusted by the scientific community
Anti-Nucleolin [364-5] (ab136649) was first used in a scientific publication in 2012 and has been cited over 10 times in peer-reviewed journals.

Other related products
We have a range of other formats of antibody clone [364-5] also available for your convenience: ab136649, Alexa Fluor® 488 - ab154028, Alexa Fluor® 647 - ab198580, Carrier free - ab212672

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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性能和储存信息

形式

Liquid

纯化说明

ab136649 is purified by biochemical fractionation. Near homogeneity as judged by SDS-PAGE.

存储溶液

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 0.88% Sodium chloride, 0.36% HEPES

运输条件

Blue Ice

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Nucleolin also known as C23 is a multifunctional protein mainly found in the nucleolus with a molecular weight of approximately 100 kDa. It plays a mechanical role in ribosomal RNA (rRNA) synthesis and ribosome biogenesis. Nucleolin is widely expressed in proliferating cells including HeLa culture and shows higher expression in cancer cells compared to normal cells. The nucleolin protein is involved in various nucleolar processes like the modulation of chromatin structure and nucleocytoplasmic transport.

Biological function summary

The nucleolin molecular weight protein contributes to regulating cellular processes such as chromatin remodeling rRNA transcription and mRNA stability. Nucleolin often forms complexes with various other proteins and nucleic acids enhancing its functionality within the cell nucleus. Its participation in processes like DNA replication and cell proliferation shows the central role nucleolin plays at the cellular level. It influences cellular responses by binding directly to specific RNA or DNA sequences.

Pathways

The nucleolin protein engages in major cellular pathways such as the RNA processing and p53 signaling pathways. Through the RNA processing pathway it interacts with proteins like fibrillarin to assist in rRNA maturation. Moreover within the p53 signaling pathway nucleolin regulates the activity of the tumor suppressor protein p53 impacting cell cycle progression and apoptosis. These interactions highlight nucleolin's role in maintaining cellular homeostasis and response to stress.

The nucleolin protein often shows altered expression in cancers and cardiovascular diseases. In cancer nucleolin's high expression levels correlate with tumor progression and metastasis making it a potential target for cancer therapies. In cardiovascular diseases nucleolin interacts with proteins like VEGF to influence angiogenesis which affects disease progression. Understanding nucleolin's involvement in these diseases provides insight into potential therapeutic targets and interventions.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.

See full target information NCL

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文献 (18)

Recent publications for all applications. Explore the full list and refine your search

Research (Washington, D.C.) 8:0918 PubMed41049614

2025

A Circular RNA Promotes Tumor Metastasis through Stabilizing MSI2 Protein in Pancreatic Ductal Adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Zhang Li,Lanyang Gao,Yang Yang,Yue Ming,Wenrong Liu,Tingting Zhang,Zixia Ye,Fuyan Xu,Juan He,Jiao Li,Jiawei Guo,Xiaojuan Yang,Qing Zhu,Yong Peng

Biology direct 20:24 PubMed40001211

2025

Nucleolin in the cell membrane promotes Ang II-mediated VSMC phenotypic switching by regulating the AT1R internalization function : Nucleolin promotes Ang II-mediated VSMC phenotypic switching.

Applications

Unspecified application

Species

Unspecified reactive species

Li Fang,Zhijie Shen,Yinzhuang Zhang,Zhuoni Mao,Dan Huang,Chenyu Lou

Life science alliance 7: PubMed38858088

2024

The nucleolar phase of signal recognition particle assembly.

Applications

Unspecified application

Species

Unspecified reactive species

Amani Issa,Florence Schlotter,Justine Flayac,Jing Chen,Ludivine Wacheul,Manon Philippe,Lucas Sardini,Lalia Mostefa,Franck Vandermoere,Edouard Bertrand,Céline Verheggen,Denis Lj Lafontaine,Séverine Massenet

Nature communications 15:2585 PubMed38519458

2024

Predicting nuclear G-quadruplex RNA-binding proteins with roles in transcription and phase separation.

Applications

Unspecified application

Species

Unspecified reactive species

Johanna Luige,Alexandros Armaos,Gian Gaetano Tartaglia,Ulf Andersson Vang Ørom

Biology open 12: PubMed37670689

2023

CRISPR/Cas9-mediated knockout of the ubiquitin variant UbKEKS reveals a role in regulating nucleolar structures and composition.

Applications

Unspecified application

Species

Unspecified reactive species

Julie Frion,Anna Meller,Gwendoline Marbach,Dominique Lévesque,Xavier Roucou,François-Michel Boisvert

Nucleic acids research 51:3357-3374 PubMed36869663

2023

Proteomic analyses reveal new features of the box H/ACA RNP biogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Florence Schlotter,Salim Mérouani,Justine Flayac,Valentyne Kogey,Amani Issa,Maxime Dodré,Alexandra Huttin,Christiane Branlant,Edouard Bertrand,Stéphane Labialle,Franck Vandermoere,Céline Verheggen,Séverine Massenet

Theranostics 12:474-492 PubMed34976196

2022

A transcriptional biosensor to monitor single cancer cell therapeutic responses by bioluminescence microscopy.

Applications

Unspecified application

Species

Unspecified reactive species

Audrey Champagne,Pallavi Jain,Lauriane Vélot,Julie Riopel,Véronique Lefebvre,Bertrand Neveu,Frédéric Pouliot

International journal of molecular sciences 22: PubMed34299079

2021

The Alkylating Agent Methyl Methanesulfonate Triggers Lipid Alterations at the Inner Nuclear Membrane That Are Independent from Its DNA-Damaging Ability.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Ovejero,Caroline Soulet,María Moriel-Carretero

Nature communications 12:3845 PubMed34158506

2021

The Atr-Chek1 pathway inhibits axon regeneration in response to Piezo-dependent mechanosensation.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Li,Tsz Y Lo,Leann Miles,Qin Wang,Harun N Noristani,Dan Li,Jingwen Niu,Shannon Trombley,Jessica I Goldshteyn,Chuxi Wang,Shuchao Wang,Jingyun Qiu,Katarzyna Pogoda,Kalpana Mandal,Megan Brewster,Panteleimon Rompolas,Ye He,Paul A Janmey,Gareth M Thomas,Shuxin Li,Yuanquan Song

Nature communications 11:1306 PubMed32161257

2020

UBB pseudogene 4 encodes functional ubiquitin variants.

Applications

Unspecified application

Species

Unspecified reactive species

Marie-Line Dubois,Anna Meller,Sondos Samandi,Mylène Brunelle,Julie Frion,Marie A Brunet,Amanda Toupin,Maxime C Beaudoin,Jean-François Jacques,Dominique Lévesque,Michelle S Scott,Pierre Lavigne,Xavier Roucou,François-Michel Boisvert

View all publications

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Anti-Nucleolin抗体[364-5]