AB86727

Anti-Nucleolin抗体[130CT38.1.5]

Name: Nucleolin抗体[130CT38.1.5] (ab86727)| Abcam中文官网
Brand: Abcam Limited
SKU: AB86727
Price: 4421 CNY
Availability: InStock
Rating: 1 (1 reviews)

Anti-Nucleolin antibody [130CT38.1.5]

BOND RX™ Validated
了解详情

(1 Review)

(1 Publication)

Mouse Monoclonal Nucleolin antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

查看别名

Nucleolin, Protein C23, NCL

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin antibody [130CT38.1.5] (AB86727)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin antibody [130CT38.1.5] (AB86727)

IHC image of ab86727 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab86727, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Project11379****

Western blot - Anti-Nucleolin antibody [130CT38.1.5] (AB86727)

All lanes:

Western blot - Anti-Nucleolin antibody [130CT38.1.5] (ab86727) at 5 µg/mL

All lanes:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 20 µg

Secondary

All lanes:

Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed at 1/10000 dilution

Predicted band size: 76 kDa

Observed band size: 100 kDa,28 kDa

true

Exposure time: 20min

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

130CT38.1.5

亚型

IgG

不含载体蛋白

反应种属

Human

应用

IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-5 µg/mL", "WB-species-notes": "<p></p>" } } }

style

left-column

产品详情

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

style

left-column

性能和储存信息

形式

Liquid

纯度

IgG fraction

存储溶液

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Nucleolin also known as C23 is a multifunctional protein mainly found in the nucleolus with a molecular weight of approximately 100 kDa. It plays a mechanical role in ribosomal RNA (rRNA) synthesis and ribosome biogenesis. Nucleolin is widely expressed in proliferating cells including HeLa culture and shows higher expression in cancer cells compared to normal cells. The nucleolin protein is involved in various nucleolar processes like the modulation of chromatin structure and nucleocytoplasmic transport.

Biological function summary

The nucleolin molecular weight protein contributes to regulating cellular processes such as chromatin remodeling rRNA transcription and mRNA stability. Nucleolin often forms complexes with various other proteins and nucleic acids enhancing its functionality within the cell nucleus. Its participation in processes like DNA replication and cell proliferation shows the central role nucleolin plays at the cellular level. It influences cellular responses by binding directly to specific RNA or DNA sequences.

Pathways

The nucleolin protein engages in major cellular pathways such as the RNA processing and p53 signaling pathways. Through the RNA processing pathway it interacts with proteins like fibrillarin to assist in rRNA maturation. Moreover within the p53 signaling pathway nucleolin regulates the activity of the tumor suppressor protein p53 impacting cell cycle progression and apoptosis. These interactions highlight nucleolin's role in maintaining cellular homeostasis and response to stress.

The nucleolin protein often shows altered expression in cancers and cardiovascular diseases. In cancer nucleolin's high expression levels correlate with tumor progression and metastasis making it a potential target for cancer therapies. In cardiovascular diseases nucleolin interacts with proteins like VEGF to influence angiogenesis which affects disease progression. Understanding nucleolin's involvement in these diseases provides insight into potential therapeutic targets and interventions.

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.

See full target information NCL

style

left-column

文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Human reproduction (Oxford, England) 30:406-16 PubMed25475585

2014

Preventive effect of oral mucosal epithelial cell sheets on intrauterine adhesions.

Applications

Unspecified application

Species

Unspecified reactive species

Goro Kuramoto,Soichi Takagi,Ken Ishitani,Tatsuya Shimizu,Teruo Okano,Hideo Matsui

View all publications

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Nucleolin抗体[130CT38.1.5]

Anti-Nucleolin antibody [130CT38.1.5]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin antibody [130CT38.1.5] (AB86727)

Western blot - Anti-Nucleolin antibody [130CT38.1.5] (AB86727)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原