Anti-Nuclear Receptor Corepressor NCoR抗体(ab3482)
Key features and details
- Rabbit polyclonal to Nuclear Receptor Corepressor NCoR
- Suitable for: ChIP, WB, IP, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-Nuclear Receptor Corepressor NCoR抗体
参阅全部 Nuclear Receptor Corepressor NCoR 一抗 -
描述
兔多克隆抗体to核Receptor Corepressor NCoR -
宿主
Rabbit -
经测试应用
适用于: ChIP, WB, IP, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Xenopus laevis, Xenopus tropicalis -
免疫原
Synthetic peptide corresponding to Mouse Nuclear Receptor Corepressor NCoR aa 2427-2443.
Sequence:PAPLLSAQYETLSDSDD
(Peptide available asab4997) -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
- Signal Transduction
- Signaling Pathway
- Nuclear Signaling
- Nuclear Hormone Receptors
- Co-activators/co-repressors
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
- Co-activators/co-repressors
- Epigenetics and Nuclear Signaling
- Chromatin Modifying Enzymes
- Acetylation
- HDACs
- Class II / Hda1 Class
相关产品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
应用 | Ab评论 | 说明 |
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ChIP |
Use at an assay dependent concentration.
Pubmed ID: 16930818. |
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WB | (1) |
Use at an assay dependent concentration. Predicted molecular weight: 270 kDa.
By Western blot, this antibody detects a protein at ~270 kDa representing N-CoR from HeLa cell extracts and mouse RAW 264.7/primary macrophages. |
IP |
Use at an assay dependent concentration.
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IHC-P | (1) |
1/200 - 1/1000.
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ICC/IF |
1/100 - 1/500.
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说明 |
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ChIP
Use at an assay dependent concentration. Pubmed ID: 16930818. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 270 kDa. By Western blot, this antibody detects a protein at ~270 kDa representing N-CoR from HeLa cell extracts and mouse RAW 264.7/primary macrophages. |
IP
Use at an assay dependent concentration. |
IHC-P
1/200 - 1/1000. |
ICC/IF
1/100 - 1/500. |
靶标
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功能
Mediates transcriptional repression by certain nuclear receptors. Part of a complex which promotes histone deacetylation and the formation of repressive chromatin structures which may impede the access of basal transcription factors. -
序列相似性
Belongs to the N-CoR nuclear receptor corepressors family.
Contains 2 SANT domains. -
结构域
The N-terminal region contains three independent domains that are capable of mediating transcriptional repression (RD1, RD2 and RD3).
The C-terminal region contains two separate nuclear receptor-interacting domains (ID1 and ID2), each of which contains a conserved sequence referred to as the CORNR box. This motif is necessary and sufficient for binding to unligated nuclear hormone receptors, while sequences flanking the CORNR box determine the precise nuclear hormone receptor specificity. -
翻译后修饰
Ubiquitinated; mediated by SIAH2 and leading to its subsequent proteasomal degradation. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 9611 Human
- Entrez Gene: 20185 Mouse
- Entrez Gene: 54299 Rat
- Entrez Gene: 398304 Xenopus laevis
- Omim: 600849 Human
- SwissProt: O75376 Human
- SwissProt: Q60974 Mouse
- SwissProt: Q9WUB5 Rat
see all -
别名
- hN CoR antibody
- hNCoR antibody
- KIAA1047 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Receptor Corepressor NCoR antibody - ChIP Grade (ab3482)
ab3482 labelling Nuclear Receptor Corepressor NCoR in the nucleus of Mouse breast tissue (right) compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Tissue was blocked in 3% H2O2-methanol for 15 min at room temperature, then incubated with primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Receptor Corepressor NCoR antibody - ChIP Grade (ab3482)
ab3482 labelling Nuclear Receptor Corepressor NCoR in the nucleus of Human breast carcinoma (right) compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Tissue was blocked in 3% H2O2-methanol for 15 min at room temperature, then incubated with primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
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Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Receptor Corepressor NCoR antibody - ChIP Grade (ab3482)
ab3482 labelling Nuclear Receptor Corepressor NCoR (green) in the nucleus and cytoplasm of MCF-7 cells by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4 ºC. A DyLight-conjugated anti-rabbit was used as the secondary antibody. Red (phalloidin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.
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Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Receptor Corepressor NCoR antibody - ChIP Grade (ab3482)
ab3482 labelling Nuclear Receptor Corepressor NCoR (green) in the nucleus and cytoplasm of HeLa cells by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4 ºC. A DyLight-conjugated anti-rabbit was used as the secondary antibody. Red (phalloidin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (15)
ab3482 被引用在 15 文献中.
- Qin D et al. Lgr5+ cell fate regulation by coordination of metabolic nuclear receptors during liver repair. Theranostics 12:6130-6142 (2022). PubMed: 36168631
- Wang Y et al. Discovery of LAMP-2A as potential biomarkers for glioblastoma development by modulating apoptosis through N-CoR degradation. Cell Commun Signal 19:40 (2021). PubMed: 33761934
- Garg M et al. Cardiolipin-mediated PPAR? S112 phosphorylation impairs IL-10 production and inflammation resolution during bacterial pneumonia. Cell Rep 34:108736 (2021). PubMed: 33567272
- Beilner D et al. Nuclear receptor corepressor (NCoR) is a positive prognosticator for cervical cancer. Arch Gynecol Obstet 304:1307-1314 (2021). PubMed: 33861372
- St-Jean S et al. NCOR1 Sustains Colorectal Cancer Cell Growth and Protects against Cellular Senescence. Cancers (Basel) 13:N/A (2021). PubMed: 34503224