Anti-NUCB2抗体(ab224348)
Key features and details
- Rabbit polyclonal to NUCB2
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-NUCB2抗体
参阅全部 NUCB2 一抗 -
描述
兔多克隆抗体to NUCB2 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment corresponding to Human NUCB2 aa 300 to the C-terminus.
Database link: P80303 -
阳性对照
- WB: HL-60 and NBT-II cell lysate. IHC-P: Human stomach tissue. ICC/IF: U-2 OS cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab224348于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 50 kDa.
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| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
| 说明 |
|---|
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IHC-P
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 50 kDa. |
|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
靶标
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功能
Calcium-binding protein. May have a role in calcium homeostasis. -
组织特异性
Predominantly expressed in spleen, testis and normal stomach. -
序列相似性
Belongs to the nucleobindin family.
Contains 2 EF-hand domains. -
细胞定位
Membrane. Cytoplasm. Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 4925 Human
- Entrez Gene: 53322 Mouse
- Entrez Gene: 59295 Rat
- Omim: 608020 Human
- SwissProt: P80303 Human
- SwissProt: P81117 Mouse
- SwissProt: Q9JI85 Rat
- Unigene: 654599 Human
see all -
别名
- DNA binding protein NEFA antibody
- DNA-binding protein NEFA antibody
- Gastric cancer antigen Zg4 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUCB2 antibody (ab224348)
Paraffin-embedded human stomach tissue stained for NUCB2 using ab224348 at 1/1000 in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-NUCB2 antibody (ab224348)PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for NUCB2 (green) using ab224348 at 4 µg/ml in ICC/IF.
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Western blot - Anti-NUCB2 antibody (ab224348)All lanes : Anti-NUCB2 antibody (ab224348) at 1/100 dilution
Lane 1 : NIH/3T3 (mouse embryonic fibroblast cell line) cell lysate
Lane 2 : NBT-II cell lysate
Developed using the ECL technique.
Predicted band size: 50 kDa -
Western blot - Anti-NUCB2 antibody (ab224348)Anti-NUCB2 antibody (ab224348) at 1/100 dilution + HL-60 (human promyelocytic leukemia cell line) cell lysate
Developed using the ECL technique.
Predicted band size: 50 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab224348 被引用在 3 文献中.
- Cao X et al. WITHDRAWN: Ethyl acetate extracted from the chinese medicinal plant Caesalpinia sappan L alleviates gustatory response in diabetes mellitus rats. Histol Histopathol N/A:18676 (2023). PubMed: 37997936
- Tao R et al. Nucleobindin-2 enhances the epithelial-mesenchymal transition in renal cell carcinoma. Oncol Lett 19:3653-3664 (2020). PubMed: 32391090
- Zhao J et al. High expression of NUCB2 promotes papillary thyroid cancer cells proliferation and invasion. Onco Targets Ther 12:1309-1318 (2019). PubMed: 30863097