Anti-NTH1抗体[2660C1a] (ab70726)
![Western blot - Anti-NTH1 antibody [2660C1a] (ab70726)](https://www.abcam.cn/ps/products/70/ab70726/Images/ab70726-85514-anti-nth1-antibody-2660c1a-western-blot.jpg "Western blot - Anti-NTH1 antibody [2660C1a] (ab70726)")
Key features and details
- Mouse monoclonal [2660C1a] to NTH1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
概述
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产品名称
Anti-NTH1抗体[2660C1a]
参阅全部 NTH1 一抗 -
描述
小鼠单克隆抗体[2660C1a] to NTH1 -
宿主
Mouse -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant N-terminal fragment of human NTH1
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阳性对照
- Recombinant human NTH1. Whole cell lysates from human HEK293, mouse NIH3T3 and rat F2408 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
纯度
Protein G purified -
纯化说明
ab70726 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane. -
克隆
单克隆 -
克隆编号
2660C1a -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab70726于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (3) |
Use a concentration of 0.2 - 2 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 34 kDa).
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| 说明 |
|---|
|
WB
Use a concentration of 0.2 - 2 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 34 kDa). |
靶标
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功能
Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site. Required for the repair of both oxidative DNA damage and spontaneous mutagenic lesions. -
组织特异性
Widely expressed with highest levels in heart and lowest levels in lung and liver. -
序列相似性
Belongs to the Nth/MutY family. -
发展阶段
Expression levels are regulated during the cell cycle with increased levels during early and mid S-phase. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 4913 Human
- Entrez Gene: 18207 Mouse
- Entrez Gene: 29541 Rat
- Omim: 602656 Human
- SwissProt: P78549 Human
- SwissProt: O35980 Mouse
- Unigene: 66196 Human
- Unigene: 148315 Mouse
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别名
- Bifunctional DNA N glycoslyase/DNA (apurinic or apyrimidinic site) lyase antibody
- DNA glycoslyase/AP lyase antibody
- Endonuclease III like protein 1 antibody
see all
图片
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Western blot - Anti-NTH1 antibody [2660C1a] (ab70726)All lanes : Anti-NTH1 antibody [2660C1a] (ab70726) at 0.2 µg/ml
Lane 1 : Whole cell lysate from human HEK293 cells
Lane 2 : Whole cell lysate from mouse NIH3T3 cells
Lane 3 : Whole cell lysate from rat F2408 cells
Lysates/proteins at 25 µg per lane.
Predicted band size: 34 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted? -
![Western blot - Anti-NTH1 antibody [2660C1a] (ab70726)](https://www.abcam.cn/ps/products/70/ab70726/Images/ab70726-85513-anti-nth1-antibody-2660c1a-western-blot.gif)
Western blot - Anti-NTH1 antibody [2660C1a] (ab70726)Anti-NTH1 antibody [2660C1a] (ab70726) at 0.2 µg/ml + immunised recombinant human NTH1 protein
Predicted band size: 34 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
实验方案
数据表及文件
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Datasheet download
文献 (3)
ab70726 被引用在 3 文献中.
- Konis SMR et al. TRIM26 Maintains Cell Survival in Response to Oxidative Stress through Regulating DNA Glycosylase Stability. Int J Mol Sci 23:N/A (2022). PubMed: 36232914
- Williams SC & Parsons JL NTH1 Is a New Target for Ubiquitylation-Dependent Regulation by TRIM26 Required for the Cellular Response to Oxidative Stress. Mol Cell Biol 38:N/A (2018). PubMed: 29610152
- Limpose KL et al. Overexpression of the base excision repair NTHL1 glycosylase causes genomic instability and early cellular hallmarks of cancer. Nucleic Acids Res 46:4515-4532 (2018). PubMed: 29522130
