AB255903

重组Anti-NTF2抗体[5A3] - BSA and Azide free

Anti-NTF2 antibody [5A3] - BSA and Azide free

Recombinant
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(1 Publication)

Mouse Recombinant Monoclonal NTF2 antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human, Rat, Mouse samples. Cited in 1 publication.

查看别名

NTF2, NUTF2, Nuclear transport factor 2, NTF-2, Placental protein 15, PP15

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-NTF2 antibody [5A3] - BSA and Azide free (AB255903)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-NTF2 antibody [5A3] - BSA and Azide free (AB255903)

This data was produced using the same clone but in a different formulation, ab254146.

Immunocytochemical analysis of 4% paraformaldehyde-fixed, 100% methanol-permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling NTF2 with ab254146 at 1/50 dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear and cytoplasmic staining in HeLa cells. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 594) at a 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue).
Negative control 1 : ab254146 at a 1/50 dilution followed by ab150080 at a 1/200 dilution.
Negative control 2 : ab179513 at a 1/200 dilution followed by ab150113 at a 1/1000 dilution.

Lab

Western blot - Anti-NTF2 antibody [5A3] - BSA and Azide free (AB255903)

This data was produced using the same clone but in a different formulation, ab254146.

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-NTF2 antibody [5A3] (<a href='/products/primary-antibodies/ntf2-antibody-5a3-ab254146'>ab254146</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 10 µg

Lane 2:

HEK-293 (human embryonic kidney epithelial cell), whole cell lysate at 10 µg

Lane 3:

HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 10 µg

Lane 4:

K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate at 10 µg

Lane 5:

IMR-32 (human neuroblastoma neuroblast), whole cell lysate at 10 µg

Lane 6:

F9 (mouse embryonal carcinoma epithelial cell), whole cell lysate at 10 µg

Lane 7:

L6 (rat skeletal muscle myoblast), whole cell lysate at 10 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 14 kDa

false

Exposure time: 8s

不同偶联物与剂型 (1)

Unconjugated

Anti-NTF2 antibody [5A3]

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

5A3

亚型

IgG2a

不含载体蛋白

Yes

反应种属

Mouse, Rat, Human

应用

WB, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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产品详情

ab255903 is the carrier-free version of ab254146.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The NTF2 protein also known as Nuclear Transport Factor 2 has an important role in the transport mechanism between the cytoplasm and the nucleus. It has a molecular weight of approximately 14 kDa. NTF2 exists in multiple tissues including in the liver and brain. This protein facilitates the transport of molecules specifically enzymes and regulatory proteins through the nuclear pore complex.

Biological function summary

The NTF2 protein acts as a transport receptor that primarily mediates the import of RanGDP into the nucleus. It forms complexes with other proteins to ensure efficient transport functionality. The interaction with Ran a small GTPase is critical in maintaining the gradient necessary for nucleocytoplasmic transport. NTF2 operates by binding to nuclear pore complexes and guiding proteins into the nucleus.

Pathways

NTF2 holds importance in nucleocytoplasmic transport and Ran-mediated transport pathways. It interacts closely with other molecules such as Ran and importin β facilitating efficient nuclear import cycles. This protein also plays an associated role in cellular processes like RNA export and signal transduction by influencing how proteins translocate across the nuclear membrane.

NTF2 connects to certain neurological disorders including Alzheimer's disease. Abnormalities in nucleocytoplasmic transport can influence disease pathology and NTF2's malfunction may lead to the accumulation of misfolded proteins impacting neural function. Additionally NTF2 involvement can be observed in cancer where changes in transport capacity may lead to altered cell cycle regulation. In cancer pathology it relates to proteins like p53 which when dysregulated promotes tumorigenesis.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Mediates the import of GDP-bound RAN from the cytoplasm into the nucleus which is essential for the function of RAN in cargo receptor-mediated nucleocytoplasmic transport. Thereby, plays indirectly a more general role in cargo receptor-mediated nucleocytoplasmic transport. Interacts with GDP-bound RAN in the cytosol, recruits it to the nuclear pore complex via its interaction with nucleoporins and promotes its nuclear import.

See full target information NUTF2

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular biology of the cell 11:703-19 PubMed10679025

2000

Monoclonal antibodies to NTF2 inhibit nuclear protein import by preventing nuclear translocation of the GTPase Ran.

Applications

Unspecified application

Species

Unspecified reactive species

S M Steggerda,B E Black,B M Paschal

View all publications

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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重组Anti-NTF2抗体[5A3] - BSA and Azide free