AB272616

Anti-NSUN3抗体

Name: NSUN3抗体 (ab272616)| Abcam中文官网
Brand: Abcam Limited
SKU: AB272616
Price: 5965 CNY
Availability: InStock

Anti-NSUN3 antibody

Be the first to review this product! Submit a review

(5 Publications)

Rabbit Polyclonal NSUN3 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NSUN3 aa 100-250.

查看别名

MSTP077, UG0651E06, NSUN3, NOL1/NOP2/Sun domain family member 3

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSUN3 antibody (AB272616)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSUN3 antibody (AB272616)

Paraffin-embedded formalin-fixed Human urinary bladder tissue stained for NSUN3 using ab272616 at 1/200 in immunohistochemical analysis.

Unknown

Western blot - Anti-NSUN3 antibody (AB272616)

All lanes:

Western blot - Anti-NSUN3 antibody (ab272616) at 0.4 µg/mL

Lane 1:

Control (vector only transfected HEK-293T lysate)

Lane 2:

NSUN3 over-expression lysate (mammalian HEK-293T cells)

Predicted band size: 38 kDa

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

WB, IHC-P

applications

免疫原

Recombinant Fragment Protein within Human NSUN3 aa 100-250. The exact immunogen used to generate this antibody is proprietary information.

Q9H649

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The NSUN3 protein also known as NOP2/Sun domain family member 3 functions as an RNA methyltransferase. It specifically catalyzes the methylation of cytosine in mitochondrial tRNA molecules which is a critical step for proper mitochondrial protein translation. NSUN3 is approximately 60 kDa in mass. It expresses mostly in tissues with high energy demands such as heart and muscle tissues due to its role in mitochondrial function.

Biological function summary

The function of NSUN3 is tightly linked to mitochondrial ribosome structure and translation. By methylating tRNA at cytosine-34 NSUN3 participates in the formation of the anticodon wobble position which is vital for accurate and efficient mitochondrial protein synthesis. It does not act alone as it often functions as part of larger mitochondrial translation complexes contributing to proper mitochondrial ribosomal assembly and function.

Pathways

NSUN3 is an integral component of the mitochondrial gene expression and oxidative phosphorylation pathways. It plays a significant role in regulating mitochondrial translation by directly modifying tRNA and interacts with other proteins like TRMT10C that also participate in this modification process. These pathways are important for maintaining mitochondrial energy metabolism and production of ATP within cells.

NSUN3 deficiency associates with mitochondrial disorders that manifest as systemic energy production failures due to impaired mitochondrial translation. These deficiencies often overlap with conditions like mitochondrial myopathy or lactic acidosis. Additionally the malfunction or absence of NSUN3 can disrupt interactions with other mitochondrial proteins like MTO1 further exacerbating mitochondrial dysfunction and leading to disease progression.

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

Mitochondrial tRNA methyltransferase that mediates methylation of cytosine to 5-methylcytosine (m5C) at position 34 of mt-tRNA(Met) (PubMed : 27214402, PubMed : 27356879, PubMed : 27497299). mt-tRNA(Met) methylation at cytosine(34) takes place at the wobble position of the anticodon and initiates the formation of 5-formylcytosine (f(5)c) at this position (PubMed : 27214402, PubMed : 27356879, PubMed : 27497299). mt-tRNA(Met) containing the f(5)c modification at the wobble position enables recognition of the AUA codon in addition to the AUG codon, expanding codon recognition in mitochondrial translation (PubMed : 27356879, PubMed : 27497299).

See full target information NSUN3

style

left-column

文献 (5)

Recent publications for all applications. Explore the full list and refine your search

Clinics (Sao Paulo, Brazil) 80:100662 PubMed40279954

2025

Inhibition of NSUN3 suppresses immune escape in non-small cell lung cancer through stabilizing PD-L1 in a 5-methyladenosine dependent way.

Applications

Unspecified application

Species

Unspecified reactive species

Hancong Huang,Xiaohong Lv,Qianhua Chen,Lixia Dong

Clinical and experimental pharmacology & physiology 52:e70026 PubMed39924309

2025

NSUN3 Aggravates Sepsis-Associated Acute Kidney Injury by Stabilising TIFA mRNA Through m5C.

Applications

Unspecified application

Species

Unspecified reactive species

Chenran Zhang,Wenming Shen,Xuwen Zheng,Ming Zhu,Kaiqi Xu,Hai Huang,Jinnan Yin

American journal of cancer research 14:1768-1783 PubMed38726285

2024

Roles of RNA mC modification patterns in prognosis and tumor microenvironment infiltration of diffuse large B-cell lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Wenting Cui,Cancan Luo,Lili Zhou,Tiantian Yu,Yongsheng Meng,Qianqian Yu,Zhixiang Lei,Ya Wang,Lijuan Peng,Qingqing Luo,Duozhuang Tang,Ruifang Sun,Li Yu

Cancer gene therapy 30:149-162 PubMed36123390

2022

YAP 5-methylcytosine modification increases its mRNA stability and promotes the transcription of exosome secretion-related genes in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjun Yu,Congcong Zhang,Yikun Wang,Xiaoting Tian,Yayou Miao,Fanyu Meng,Lifang Ma,Xiao Zhang,Jinjing Xia

Oral diseases 30:313-328 PubMed35997137

2022

RNA 5-Methylcytosine Regulator NSUN3 promotes tumor progression through regulating immune infiltration in head and neck squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Shufang Jin,Jiayi Li,Yihan Shen,Yiqun Wu,Zhiyuan Zhang,Hailong Ma

View all publications

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NSUN3抗体

Anti-NSUN3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSUN3 antibody (AB272616)

Western blot - Anti-NSUN3 antibody (AB272616)