Anti-NSP1抗体[32D6] (ab4641)
![Immunocytochemistry/ Immunofluorescence - Anti-NSP1 antibody [32D6] (ab4641)](https://www.abcam.cn/ps/products/4/ab4641/Images/Nsp1p-Primary-antibodies-ab4641-5.jpg "Immunocytochemistry/ Immunofluorescence - Anti-NSP1 antibody [32D6] (ab4641)")
Key features and details
- Mouse monoclonal [32D6] to NSP1
- Suitable for: ICC/IF, WB
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG
概述
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产品名称
Anti-NSP1抗体[32D6] -
描述
小鼠单克隆抗体[32D6] to NSP1 -
宿主
Mouse -
特异性
This antibody was initially identified as directed against a nuclear pore complex antigen by immunofluorescence localization. A screen of a lgt11 expression library yielded a single positive clone carrying an insert bearing ~66% of the C-terminal portion of NSP1. To confirm that 32D6 possessed high affinity for NSP1, strain RS453, which expresses a shortened isoform of NSP1, was compared to the wildtype strain BJ5465. NSP1 in RS453 contains an internal deletion that removes the coding sequence for 6 FXFG repeats, which are not essential for function and encodes a protein that has been observed to migrate at approxmately 85 kDa on SDS gels. The predicted size of wildtype NSP1 is 86.5 kDa, but NSP1 has been observed to migrate on SDS gels at ~100 kDa. In our gels, the apparent molecular mass of wildtype NSP1 was 108 kDa, whereas the short isoform of NSP1 in RS453 migrated at 91 kDa. The detection of two protein bands of apparent sizes 108 kDa and 91 kDa in BJ5465 and RS453, respectively, demonstrated that 32D6 recognized the pore complex protein NSP1. -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Saccharomyces cerevisiae -
免疫原
Tissue, cells or virus corresponding to NSP1. Yeast nuclear preparations
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常规说明
This antibody is a useful marker of yeast nuclear pores.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.065% Sodium azide
Constituent: Tissue culture supernatant -
Concentration information loading... -
纯度
Tissue culture supernatant -
纯化说明
Sterile filtered. -
Primary antibody说明
This antibody is a useful marker of yeast nuclear pores. -
克隆
单克隆 -
克隆编号
32D6 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (1) |
1/1000.
Yeast cells. |
| WB |
1/10000 - 1/50000. Detects a band of approximately 108,91 kDa (predicted molecular weight: 85 kDa).
1/10000 (cell lysates) - 1/50000 (nuclear fractions). For other (non-ECL) detection methods, 1/1000 - 1/5000. |
| 说明 |
|---|
|
ICC/IF
1/1000. Yeast cells. |
|
WB
1/10000 - 1/50000. Detects a band of approximately 108,91 kDa (predicted molecular weight: 85 kDa). 1/10000 (cell lysates) - 1/50000 (nuclear fractions). For other (non-ECL) detection methods, 1/1000 - 1/5000. |
靶标
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相关性
NSP1 functions as a component of the nuclear pore complex (NPC). It plays an important role in several nuclear transport pathways including poly(A)+ RNA, tRNA, pre-ribosome, signal recognition particle (SRP), and protein transport. -
细胞定位
Nuclear membrane: part of the nuclear pore complex, it presents a symmetric distribution with a cytoplasmic and a nucleoplasmic side. -
数据库链接
- Entrez Gene: 853409 Saccharomyces cerevisiae
- SwissProt: P14907 Saccharomyces cerevisiae
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别名
- NSP1 antibody
- Nsp1p antibody
- Nuclear pore protein NSP1 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-NSP1 antibody [32D6] (ab4641)Image courtesy of an anonymous Abreview.
ab4641 (1/5000) detecting NSP1 in Saccharomyces cerevisae DDY1810 (green). Cells were fixed in paraformaldehyde, permeabilized in methanol/acetone and counterstained with DAPI in order to highlight the nucleus (blue). For further experimental details please refer to Abreview.
实验方案
数据表及文件
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Datasheet download
文献 (8)
ab4641 被引用在 8 文献中.
- Bittmann J et al. An advanced cell cycle tag toolbox reveals principles underlying temporal control of structure-selective nucleases. Elife 9:N/A (2020). PubMed: 32352375
- Male G et al. Nucleolar size regulates nuclear envelope shape in Saccharomyces cerevisiae. J Cell Sci 133:N/A (2020). PubMed: 32973112
- Zhang W et al. Brr6 and Brl1 locate to nuclear pore complex assembly sites to promote their biogenesis. J Cell Biol 217:877-894 (2018). PubMed: 29439116
- Rüthnick D et al. Characterization of spindle pole body duplication reveals a regulatory role for nuclear pore complexes. J Cell Biol 216:2425-2442 (2017). PubMed: 28659328
- Colombi P et al. The transmission of nuclear pore complexes to daughter cells requires a cytoplasmic pool of Nsp1. J Cell Biol 203:215-32 (2013). WB, ICC/IF . PubMed: 24165936
- Ringel AE et al. Yeast Tdh3 (glyceraldehyde 3-phosphate dehydrogenase) is a Sir2-interacting factor that regulates transcriptional silencing and rDNA recombination. PLoS Genet 9:e1003871 (2013). ICC/IF ; Saccharomyces cerevisiae . PubMed: 24146631
- Beach A et al. Mitochondrial membrane lipidome defines yeast longevity. Aging (Albany NY) 5:551-74 (2013). PubMed: 23924582
- Motwani T et al. Sir3 and epigenetic inheritance of silent chromatin in Saccharomyces cerevisiae. Mol Cell Biol 32:2784-93 (2012). ICC/IF ; Saccharomyces cerevisiae . PubMed: 22586263
