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AB95963

Anti-NSFL1C抗体

Anti-NSFL1C antibody

4

(1 Review)

|

(3 Publications)

Rabbit Polyclonal NSFL1C antibody. Suitable for WB, IHC-P, IP and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NSFL1C aa 1-300.

查看别名

UBXN2C, NSFL1C, NSFL1 cofactor p47, UBX domain-containing protein 2C, p97 cofactor p47

2 Images
Western blot - Anti-NSFL1C antibody (AB95963)
  • WB

Supplier Data

Western blot - Anti-NSFL1C antibody (AB95963)

Samples were separated by 10% SDS PAGE.

All lanes:

Western blot - Anti-NSFL1C antibody (ab95963) at 1/1000 dilution

All lanes:

Mouse brain whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 41 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSFL1C antibody (AB95963)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSFL1C antibody (AB95963)

ab95963, at 1/100, staining paraffin-embedded differential N/T pair Lung SCC cancer by Immunohistochemistry.

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Mouse, Human

应用

IHC-P, WB, IP

applications

免疫原

Recombinant Fragment Protein within Human NSFL1C aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

Q9UNZ2

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搭配使用产品

Primary Antibodies

AB181047

Anti-MMS22L (C6ORF167) antibody [EPR13654]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-MMS22L (C6ORF167) antibody [EPR13654] (AB181047)

  • 4
  • 2
Primary Antibodies

AB32072

Anti-c-Myc antibody [Y69] - ChIP Grade

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myc antibody [Y69] - ChIP Grade (AB32072)

  • 4
  • 56
Primary Antibodies

AB58623

Anti-NFS1 antibody [2635E1a]

  • 0
  • 0
Primary Antibodies

AB199489

Anti-MAX antibody [EPR19352] - ChIP Grade

20ul selling size|RabMAb|Advanced Validation|Recombinant

ChIP-sequencing - Anti-MAX antibody [EPR19352] - ChIP Grade (AB199489)

  • 5
  • 3
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" } } }
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
存储溶液
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

NSFL1C also known as p97 cofactor p47 is a protein involved in cellular functions related to NSF ATPases. The protein has a mass of approximately 47 kDa. NSFL1C is expressed in various tissues throughout the body where it plays an active role in modulating interactions between cellular structures. This protein mainly functions by regulating membrane fusion events and protein degradation working closely with other components to maintain cellular integrity.
Biological function summary

NSFL1C acts as an important modulator in cellular homeostasis often participating in complexes with other proteins like p97/VCP a well-known AAA ATPase. This partnership allows NSFL1C to influence diverse cellular activities including endoplasmic-reticulum-associated protein degradation (ERAD) ensuring the removal of misfolded proteins. Therefore NSFL1C helps maintain protein quality control a critical cellular process that prevents accumulation of dysfunctional proteins.

Pathways

NSFL1C connects closely with the ubiquitin-proteasome pathway and autophagy process. Through its association with p97/VCP NSFL1C facilitates the disassembly and recycling of ubiquitinated proteins highlighting its role in cellular proteostasis. Another key pathway involves its cooperation with complexes involved in membrane trafficking linking NSFL1C to cellular transport mechanisms. These associations enable it to coordinate the removal of defective proteins and contribute to regular cellular operations.

NSFL1C alterations contribute to the pathology of neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS) and inclusion body myopathy. Mutations in NSFL1C or its partner protein p97/VCP have been implicated in these conditions leading to impaired clearance of misfolded proteins and disrupted cellular functions. An understanding of NSFL1C's role in these disorders highlights the importance of investigating therapeutic strategies targeting protein quality control mechanisms.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Reduces the ATPase activity of VCP (By similarity). Necessary for the fragmentation of Golgi stacks during mitosis and for VCP-mediated reassembly of Golgi stacks after mitosis (By similarity). May play a role in VCP-mediated formation of transitional endoplasmic reticulum (tER) (By similarity). Inhibits the activity of CTSL (in vitro) (PubMed : 15498563). Together with UBXN2B/p37, regulates the centrosomal levels of kinase AURKA/Aurora A during mitotic progression by promoting AURKA removal from centrosomes in prophase (PubMed : 23649807). Also, regulates spindle orientation during mitosis (PubMed : 23649807).
See full target information NSFL1C
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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Cell chemical biology 23:517-28 PubMed27105284

2016

p97 Composition Changes Caused by Allosteric Inhibition Are Suppressed by an On-Target Mechanism that Increases the Enzyme's ATPase Activity.

Applications

Unspecified application

Species

Unspecified reactive species

Nam-Gu Her,Julia I Toth,Chen-Ting Ma,Yang Wei,Khatereh Motamedchaboki,Eduard Sergienko,Matthew D Petroski

Nature structural & molecular biology 19:1093-100 PubMed23042607

2012

DVC1 (C1orf124) recruits the p97 protein segregase to sites of DNA damage.

Applications

WB

Species

Human

Emily J Davis,Christophe Lachaud,Paul Appleton,Thomas J Macartney,Inke Näthke,John Rouse

Nature structural & molecular biology 19:1084-92 PubMed23042605

2012

DVC1 (C1orf124) is a DNA damage-targeting p97 adaptor that promotes ubiquitin-dependent responses to replication blocks.

Applications

WB

Species

Human

Anna Mosbech,Ian Gibbs-Seymour,Konstantinos Kagias,Tina Thorslund,Petra Beli,Lou Povlsen,Sofie Vincents Nielsen,Stine Smedegaard,Garry Sedgwick,Claudia Lukas,Rasmus Hartmann-Petersen,Jiri Lukas,Chunaram Choudhary,Roger Pocock,Simon Bekker-Jensen,Niels Mailand
View all publications
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