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Anti-NSE抗体- Neuronal Marker (ab834)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody - Neuronal Marker (ab834)

    Key features and details

    • Rabbit polyclonal to NSE - Neuronal Marker
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG

    选择批间可重复性更高的重组抗体

    Product image
    Anti-NSE antibody [EPR3377] - BSA and Azide free (ab220216)
    • 研究可靠 —— 各批次间结果一致且可重复
    • 长期批量供应 —— 采用重组技术，可实现快速生产
    • 首次实验即可成功 —— 经过大量验证确认了特异性
    • 符合伦理标准 —— 产品不含动物成分

    概述

    • 产品名称

      Anti-NSE抗体- Neuronal Marker
      参阅全部 NSE 一抗

    • 描述

      兔多克隆抗体to NSE - Neuronal Marker

    • 宿主

      Rabbit

    • 特异性

      This antibody reacts with human isoenzymes of neuron specific enolase containing gamma subunits. This antibody gives a specific staining in neurons of the brain, in islets of langerhans in pancreas and in peripheral nerves of the skin. No staining with other tissue types has been seen.

    • 经测试应用

      适用于: IHC-Pmore details

    • 种属反应性

      与反应: Human

    • 免疫原

      Full length native protein (purified) corresponding to Bovine NSE. Neuron specific enolase (gamma-gamma isoenzyme) isolated from bovine brain.

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab834于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    IHC-P
    说明
    应用说明
    IHC: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method.
    Staining Protocol: We suggest an incubation period of 30-60 minutes at room temperature.
    However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user.

    靶标

    • 功能

      Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.

    • 组织特异性

      The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.

    • 通路

      Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.

    • 序列相似性

      Belongs to the enolase family.

    • 发展阶段

      During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.

    • 细胞定位

      Cytoplasm. Cell membrane. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form.
    • Information by UniProt

    • 数据库链接

      • 别名

        • 2 phospho D glycerate hydrolyase antibody
        • 2-phospho-D-glycerate hydro-lyase antibody
        • Eno 2 antibody
        • ENO2 antibody
        • ENOG antibody
        • ENOG_HUMAN antibody
        • Enolase 2 (gamma, neuronal) antibody
        • Enolase 2 antibody
        • Enolase 2 gamma neuronal antibody
        • Enolase2 antibody
        • Epididymis secretory protein Li 279 antibody
        • Gamma enolase antibody
        • Gamma-enolase antibody
        • HEL S 279 antibody
        • Neural enolase antibody
        • Neuron specific enolase antibody
        • Neuron specific gamma enolase antibody
        • Neuron-specific enolase antibody
        • neuronal enriched enolase antibody
        • Neurone specific enolase antibody
        • NSE antibody
        see all

      图片

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody - Neuronal Marker (ab834)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody - Neuronal Marker (ab834)
        Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue, staining NSE - Neuronal Marker with ab834.

      文献 (1)

      发表研究结果有使用 ab834？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab834 被引用在 1 文献中.

      • Tang YY  et al. Ginsenoside Rg1 Promotes the Migration of Olfactory Ensheathing Cells via the PI3K/Akt Pathway to Repair Rat Spinal Cord Injury. Biol Pharm Bull 40:1630-1637 (2017). PubMed: 28966235

      客户评价及客户问答

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      Question

      Is NSE sensitive to triton-X? Should I be leaving this out of my IHC protocol, or can I leave it in?

      Read More
      Answer

      We recommend the use of 0.025% Triton in the TBS as it helps to reduce surface tension, allowing reagents to cover the whole tissue section with ease. It is also believed to dissolve Fc receptors, therefore reducing non-specific binding. Furthermore, TBS gives a cleaner background than PBS. Care must be taken using Triton when working with membrane-bound proteins as the Triton acts as a surfactant and strips the lipid component out of the membrane. Neuron specific enolase (NSE) is located in the cytoplasm of mature neurons so you should have no problem using Triton in your buffer.

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