重组Anti-NSE抗体[EPR12483] (ab180943)
![Immunocytochemistry/ Immunofluorescence - Anti-NSE antibody [EPR12483] (ab180943)](https://www.abcam.cn/ps/products/180/ab180943/Images/ab180943-6-anti-nse-antibody-epr12483-immunocytochemistry-pc12-rat.jpg "Immunocytochemistry/ Immunofluorescence - Anti-NSE antibody [EPR12483] (ab180943)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12483] to NSE
- Suitable for: Flow Cyt (Intra), ICC/IF, WB, IP
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-NSE抗体[EPR12483]
参阅全部 NSE 一抗 -
描述
兔单克隆抗体[EPR12483] to NSE -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), ICC/IF, WB, IPmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat
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免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Fetal brain, HeLa, SH-SY5Y, U87-MG and HepG2 whole cell lysate (ab7900); U87-MG cells. ICC/IF: NIH/3T3, Ramos and Raji cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR12483 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab180943于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/10.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| ICC/IF |
1/100.
|
|
| WB |
1/1000 - 1/10000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
|
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| IP |
1/50.
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| 说明 |
|---|
|
Flow Cyt (Intra)
1/10. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
ICC/IF
1/100. |
|
WB
1/1000 - 1/10000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa). |
|
IP
1/50. |
靶标
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功能
Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival. -
组织特异性
The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons. -
通路
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5. -
序列相似性
Belongs to the enolase family. -
发展阶段
During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells. -
细胞定位
Cytoplasm. Cell membrane. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. - Information by UniProt
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数据库链接
- Entrez Gene: 2026 Human
- Entrez Gene: 13807 Mouse
- Entrez Gene: 24334 Rat
- Omim: 131360 Human
- SwissProt: P09104 Human
- SwissProt: P17183 Mouse
- SwissProt: P07323 Rat
- Unigene: 511915 Human
see all -
别名
- 2 phospho D glycerate hydrolyase antibody
- 2-phospho-D-glycerate hydro-lyase antibody
- Eno 2 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-NSE antibody [EPR12483] (ab180943)
Immunocytochemistry/ Immunofluorescence analysis of NIH/3T3 (mouse embryonic fibroblast) cells labeling alpha smooth muscle Actin with purified ab150301 at 1/100(1.65 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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![Immunocytochemistry/ Immunofluorescence - Anti-NSE antibody [EPR12483] (ab180943)](https://www.abcam.cn/ps/products/180/ab180943/Images/ab180943-5-anti-nse-antibody-epr12483-immunocytochemistry-neuro2a-mouse.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-NSE antibody [EPR12483] (ab180943)Immunocytochemistry/ Immunofluorescence analysis of Ramos (human Burkitt's lymphoma B lymphocyte) cells labeling Tcl1 with purified ab225718 at 1/50 (2.6 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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![Immunocytochemistry/ Immunofluorescence - Anti-NSE antibody [EPR12483] (ab180943)](https://www.abcam.cn/ps/products/180/ab180943/Images/ab180943-4-anti-nse-antibody-epr12483-immunocytochemistry-hela-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-NSE antibody [EPR12483] (ab180943)Immunocytochemistry/ Immunofluorescence analysis of Raji (human Burkitt's lymphoma B lymphocyte) cells labeling CD23 with purified ab135386 at 1/25 (7.48 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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![Western blot - Anti-NSE antibody [EPR12483] (ab180943)](https://www.abcam.cn/ps/products/180/ab180943/Images/ab180943-212412-anti-nse-antibody-epr12483-western-blot.jpg)
Western blot - Anti-NSE antibody [EPR12483] (ab180943)All lanes : Anti-NSE antibody [EPR12483] (ab180943) at 1/5000 dilution
Lane 1 : Fetal brain lysate
Lane 2 : HeLa cell lysate
Lane 3 : SH-SY5Y cell lysate
Lane 4 : U87-MG cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDa -
![Flow Cytometry (Intracellular) - Anti-NSE antibody [EPR12483] (ab180943)](https://www.abcam.cn/ps/products/180/ab180943/Images/ab180943-212413-anti-nse-antibody-epr12483-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-NSE antibody [EPR12483] (ab180943)Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed U87-MG cells labeling NSE with ab180943 at 1/10 dilution (red) compared to a Rabbit monoclonal IgG Isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
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![Immunoprecipitation - Anti-NSE antibody [EPR12483] (ab180943)](https://www.abcam.cn/ps/products/180/ab180943/Images/ab180943-212414-anti-nse-antibody-epr12483-immunoprecipitation.jpg)
Immunoprecipitation - Anti-NSE antibody [EPR12483] (ab180943)Western blot analysis of HepG2 cell lysate immunoprecipitated using ab180943 at 1/50 dilution (Lane 1). Lane 2: Negative control. Anti-Rabbit IgG (HRP) secondary antibody, specific to the non-reduced form of IgG, used at 1/1500 dilution.
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![Anti-NSE antibody [EPR12483] (ab180943)](https://www.abcam.cn/ps/products/180/ab180943/Images/ab180943-7-benefits-of-recombinant-antibodies.png)
Anti-NSE antibody [EPR12483] (ab180943)
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (9)
ab180943 被引用在 9 文献中.
- Yin L et al. AR antagonists develop drug resistance through TOMM20 autophagic degradation-promoted transformation to neuroendocrine prostate cancer. J Exp Clin Cancer Res 42:204 (2023). PubMed: 37563661
- He XY et al. Magnetic resonance imaging focused on the ferritin heavy chain 1 reporter gene detects neuronal differentiation in stem cells. Neural Regen Res 18:1563-1569 (2023). PubMed: 36571363
- Ji S et al. miR-145-5p Inhibits Neuroendocrine Differentiation and Tumor Growth by Regulating the SOX11/MYCN Axis in Prostate cancer. Front Genet 13:790621 (2022). PubMed: 35368699
- Yang T et al. TTF1 suppresses neuroblastoma growth and induces neuroblastoma differentiation by targeting TrkA and the miR-204/TrkB axis. iScience 25:104655 (2022). PubMed: 35811845
- Zhang F et al. Blood-brain barrier permeability to enrofloxacin in Pengze crucian carp (Carassius auratus var. Pengze). Vet Med Sci 8:2404-2410 (2022). PubMed: 36037402
- Feng L et al. Transmembrane p24 trafficking protein 2 regulates inflammation through the TLR4/NF-?B signaling pathway in lung adenocarcinoma. World J Surg Oncol 20:32 (2022). PubMed: 35135563
- Xu Y et al. Green tea protects against hippocampal neuronal apoptosis in diabetic encephalopathy by inhibiting JNK/MLCK signaling. Mol Med Rep 24:N/A (2021). PubMed: 34132368
- Lin H et al. Brain-derived extracellular vesicles mediated coagulopathy, inflammation and apoptosis after sepsis. Thromb Res 207:85-95 (2021). PubMed: 34583153
- Deng Y et al. SOX9 Knockdown-Mediated FOXO3 Downregulation Confers Neuroprotection Against Ischemic Brain Injury. Front Cell Dev Biol 8:555175 (2020). PubMed: 33791290