Anti-NRG1 type III抗体(ab23248)
Key features and details
- Rabbit polyclonal to NRG1 type III
- Suitable for: IHC-Fr, WB
- Reacts with: Mouse, Rat
- Isotype: IgG
概述
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产品名称
Anti-NRG1 type III抗体 -
描述
兔多克隆抗体to NRG1 type III -
宿主
Rabbit -
特异性
ab23248 detects ~50-55kDa band in rat sciatic nerve, rat and mouse brain lysate. IHC detection with ab23248 in mouse DRG shows a primarily nuclear localisation of the protein rather than the expected cytoplasmic staining (See Bao and Wang references). -
经测试应用
适用于: IHC-Fr, WBmore details -
种属反应性
与反应: Mouse, Rat
预测可用于: Chicken, Human
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免疫原
Synthetic peptide corresponding to Human NRG1 type III aa 50-150 (internal sequence) conjugated to keyhole limpet haemocyanin.
(Peptide available asab23378) -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab23248于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-Fr | (1) |
Use a concentration of 0.34 µg/ml.
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| WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa.
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| 说明 |
|---|
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IHC-Fr
Use a concentration of 0.34 µg/ml. |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa. |
靶标
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相关性
NRG1 type III is a sensory and motor neuron-derived factor isoform of Neuregulin 1, specifically expressed in the nervous system: spinal cord motor neurons, dorsal root ganglion neurons, and brain. NRG1 type III is the predominant isoform expressed in sensory and motor neurons. The Nrg1 gene encodes more than 15 transmembrane and secreted protein isoforms, generated by alternative promoter usage and mRNA splicing. Nrg1 subtypes I through III share the epidermal growth factor-like signaling domain and are defined by different amino-termini. The N-terminal cysteine-rich domain (CRD), as found in the sensory and motor neuronderived factor (SMDF), defines Nrg1 type III, which in embryonic development is responsible for survival of Schwann cell precursors. -
细胞定位
Cell Membrane and Secreted -
数据库链接
- Entrez Gene: 3084 Human
- Entrez Gene: 211323 Mouse
- Entrez Gene: 112400 Rat
- SwissProt: Q02297 Human
- SwissProt: Q15491 Human
- SwissProt: P43322 Rat
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别名
- Heregulin antibody
- HRG antibody
- HRG1 antibody
see all
图片
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Immunohistochemistry (Frozen sections) - Anti-NRG1 type III antibody (ab23248)This image is courtesy of David Bennett, Kings College London, United Kingdom
ab23248 detecting NRG1 Type III in mouse L4/5 dorsal root ganglion (DRG) tissue (perfused with 4% paraformaldehyde and cryoprotected overnight, cut on a cryostat at 15µm). Tissue was incubated in blocking serum (normal goat serum) for 1hr, washed 3XPBS before primary antibody incubation in PBS with triton (0.1%) overnight (ab23248 was used at 1/800 = 0.34µg/ml). Secondary antibody used was goat anti-rabbit Alexa 546 (1/1000). The image shown is taken with a X20 objective and shows mostly nuclear NRG1 Type III staining with some cytomplasmic staining (nuclear counterstain with DAPI confirmed this - not shown).
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Western blot - Anti-NRG1 type III antibody (ab23248)All lanes : Anti-NRG1 type III antibody (ab23248) at 1 µg/ml
Lane 1 : Mouse Sciatic Nerve Lysate
Lane 2 : Mouse Brain
Lane 3 : Brain (Rat) Tissue Lysate (ab7942)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Rabbit IgG secondary antibody (ab28446) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 27,52,74,75 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?ab23248 picks up an ~50kDa band in WB when tested on rat sciatic nerve, rat brain or mouse brain. This band size concurrs with an entry for NRG1 in both Expasy and NCBI databases (Q3URY7). Carroll et al., (J Neurosci. 1997 Mar 1;17(5):1642-59) also detect a 50kDa band in dorsal root ganglion and spinal cord. However, it is surprising that we do not detect more bands; it is likely that ab23248 does not pick up all NRG1 type III isoforms or that there is very restricted expression in the tissues tested here.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab23248 被引用在 7 文献中.
- Tan C et al. Effect of Schwann cell transplantation combined with electroacupuncture on axonal regeneration and remyelination in rats with spinal cord injury. Anat Rec (Hoboken) 304:2506-2520 (2021). PubMed: 34319000
- Exposito-Alonso D et al. Subcellular sorting of neuregulins controls the assembly of excitatory-inhibitory cortical circuits. Elife 9:N/A (2020). PubMed: 33320083
- Shea GK et al. Juxtacrine signalling via Notch and ErbB receptors in the switch to fate commitment of bone marrow-derived Schwann cells. Eur J Neurosci 52:3306-3321 (2020). PubMed: 32460437
- Chen S et al. Neuregulin-1 Accelerates Functional Motor Recovery by Improving Motoneuron Survival After Brachial Plexus Root Avulsion in Mice. Neuroscience 404:510-518 (2019). PubMed: 30731156
- Nguyen TV et al. Alzheimer's associated amyloid and tau deposition co-localizes with a homeostatic myelin repair pathway in two mouse models of post-stroke mixed dementia. Acta Neuropathol Commun 6:100 (2018). PubMed: 30249297
- Yue Y et al. Low-intensity pulsed ultrasound upregulates pro-myelination indicators of Schwann cells enhanced by co-culture with adipose-derived stem cells. Cell Prolif 49:720-728 (2016). PubMed: 27625295
- Sango K et al. Myelination in coculture of established neuronal and Schwann cell lines. Histochem Cell Biol 137:829-39 (2012). ICC/IF ; Rat . PubMed: 22366958