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重组RabMAb

重组Anti-Nrf2 (phospho S40)抗体[EP1809Y] - BSA and Azide free (ab180844)

Reviews (1) Submit a question References (8)
Flow Cytometry (Intracellular) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] - BSA and Azide free (ab180844)
  • Immunocytochemistry/ Immunofluorescence - Anti-Nrf2 (phospho S40) antibody [EP1809Y] - BSA and Azide free (ab180844)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] - BSA and Azide free (ab180844)
  • Dot Blot - Anti-Nrf2 (phospho S40) antibody [EP1809Y] - BSA and Azide free (ab180844)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] - BSA and Azide free (ab180844)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] - BSA and Azide free (ab180844)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] - BSA and Azide free (ab180844)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] - BSA and Azide free (ab180844)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] - BSA and Azide free (ab180844)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] - BSA and Azide free (ab180844)
  • Anti-Nrf2 (phospho S40) antibody [EP1809Y] - BSA and Azide free (ab180844)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1809Y] to Nrf2 (phospho S40) - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), ICC/IF, IHC-P, WB, Dot blot
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 555 Alexa Fluor® 594 Alexa Fluor® 750 APC PE Unconjugated

概述

  • 产品名称

    Anti-Nrf2 (phospho S40)抗体[EP1809Y] - BSA and Azide free
    参阅全部 Nrf2 一抗

  • 描述

    兔单克隆抗体[EP1809Y] to Nrf2 (phospho S40) - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Flow Cyt (Intra), ICC/IF, IHC-P, WB, Dot blotmore details
    不适用于: IP

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab133404)

  • 阳性对照

    • Human breast carcinoma tissue, HepG2 cell lysate

  • 常规说明

    ab180844 is the carrier-free version of ab76026.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab180844于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF
Use at an assay dependent concentration.
IHC-P (1)
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Predicted molecular weight: 68 kDa.

Can be blocked with Nrf2 (phospho S40) peptide (ab133404).
Dot blot
Use at an assay dependent concentration.
说明
Flow Cyt (Intra)
Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Predicted molecular weight: 68 kDa.

Can be blocked with Nrf2 (phospho S40) peptide (ab133404).
Dot blot
Use at an assay dependent concentration.
应用说明
Is unsuitable for IP.

靶标

  • 功能

    Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.

  • 组织特异性

    Widely expressed. Highest expression in adult muscle, kidney, lung, liver and in fetal muscle.

  • 序列相似性

    Belongs to the bZIP family. CNC subfamily.
    Contains 1 bZIP domain.

  • 结构域

    Acidic activation domain in the N-terminus, and DNA binding domain in the C-terminus.

  • 翻译后修饰

    Phosphorylation of Ser-40 by PKC in response to oxidative stress dissociates NFE2L2 from its cytoplasmic inhibitor KEAP1, promoting its translocation into the nucleus.

  • 细胞定位

    Cytoplasm > cytosol. Nucleus. Cytosolic under unstressed conditions, translocates into the nucleus upon induction by electrophilic agents.
  • Information by UniProt

  • 数据库链接

    • 别名

      • erythroid derived 2 antibody
      • HEBP1 antibody
      • like 2 antibody
      • NF E2 related factor 2 antibody
      • NF-E2-related factor 2 antibody
      • NF2L2_HUMAN antibody
      • NFE2 related factor 2 antibody
      • NFE2-related factor 2 antibody
      • Nfe2l2 antibody
      • Nrf 2 antibody
      • NRF2 antibody
      • Nuclear factor (erythroid derived 2) like 2 antibody
      • Nuclear factor antibody
      • nuclear factor erythroid 2 like 2 antibody
      • Nuclear factor erythroid 2 related factor 2 antibody
      • Nuclear factor erythroid 2-related factor 2 antibody
      • Nuclear factor erythroid derived 2 like 2 antibody
      see all

    图片

    • Flow Cytometry (Intracellular) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] - BSA and Azide free (ab180844)
      Flow Cytometry (Intracellular) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] - BSA and Azide free (ab180844)

      Overlay histogram showing Jurkat cells fixed in 4% PFA and stained with purified ab76026 at a dilution of 1 in 80 (red line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 500. Rabbit monoclonal IgG was used as an isotype control (black line) and cells incubated in the absence of both primary and secondary antibody were used as a negative control (blue line). This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76026). 

       

       

    • Immunocytochemistry/ Immunofluorescence - Anti-Nrf2 (phospho S40) antibody [EP1809Y] - BSA and Azide free (ab180844)
      Immunocytochemistry/ Immunofluorescence - Anti-Nrf2 (phospho S40) antibody [EP1809Y] - BSA and Azide free (ab180844)

      Immunofluorescence staining of HepG2 cells with purified ab76026 at a working dilution of 1/100, counter-stained with DAPI. The treated cells were treated with alkaline phosphatase for 1 h at 37°C. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab76026 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76026).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] - BSA and Azide free (ab180844)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] - BSA and Azide free (ab180844)
      Immunohistochemical staining of paraffin embedded human breast carcinoma with purified ab76026 at a working dilution of 1/500. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76026).

    • Dot Blot - Anti-Nrf2 (phospho S40) antibody [EP1809Y] - BSA and Azide free (ab180844)
      Dot Blot - Anti-Nrf2 (phospho S40) antibody [EP1809Y] - BSA and Azide free (ab180844)

      Dot blot analysis of Nrf2 peptides using unpurified ab76026 at 1/1000 dilution followed by Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated secondary antibody at 1/1000 dilution. Blocking and diluting buffer was 5% NFDM/TBST.

       

      Lane 1: Nrf2 (pS40) phospho peptide

      Lane 2: Nrf2 non-phospho peptide

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76026).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] - BSA and Azide free (ab180844)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] - BSA and Azide free (ab180844)

      Immunohistochemical analysis of paraffin-embedded human breast carcinoma using unpurified ab76026 at 1/100 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76026).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] - BSA and Azide free (ab180844)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] - BSA and Azide free (ab180844)This image is courtesy of an anonymous Abreview.

      Unpurified ab76026 staining Nrf2 (phospho S40) in Human normal lung tissue sections by IHC-P (Formaldehyde-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% casein for 30 minutes at 4°C. Antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/50) in 1% casein for 24 hours at 4°C. An undiluted HRP-conjugated Goat polyclonal to rabbit IgG was used as the secondary antibody.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76026).

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] - BSA and Azide free (ab180844)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] - BSA and Azide free (ab180844)

      Unpurified ab76026 showing positive staining in Breast carcinoma tissue. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76026).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] - BSA and Azide free (ab180844)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] - BSA and Azide free (ab180844)

      Unpurified ab76026 showing positive staining in Cervical carcinoma tissue. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76026).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] - BSA and Azide free (ab180844)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] - BSA and Azide free (ab180844)

      Unpurified ab76026 showing positive staining in Ovarian carcinoma tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76026).

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] - BSA and Azide free (ab180844)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] - BSA and Azide free (ab180844)

      Unpurified ab76026 showing positive staining in Normal tonsil tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76026).

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Anti-Nrf2 (phospho S40) antibody [EP1809Y] - BSA and Azide free (ab180844)
      Anti-Nrf2 (phospho S40) antibody [EP1809Y] - BSA and Azide free (ab180844)

    文献 (8)

    发表研究结果有使用 ab180844？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab180844 被引用在 8 文献中.

    • Wu CC  et al. Evaluation of the post-treatment anti-inflammatory capacity of osteoarthritic chondrocytes: An in vitro study using baicalein. Regen Ther 14:177-183 (2020). PubMed: 32128354
    • Ji L  et al. Nrf2 pathway regulates multidrug-resistance-associated protein 1 in small cell lung cancer. PLoS One 8:e63404 (2013). IHC-P ; Human . PubMed: 23667609
    • Bhaskaran N  et al. Induction of heme oxygenase-1 by chamomile protects murine macrophages against oxidative stress. Life Sci 90:1027-33 (2012). WB ; Mouse . PubMed: 22683429
    • Li Y  et al. Mechanism of chemical activation of Nrf2. PLoS One 7:e35122 (2012). WB . PubMed: 22558124
    • Im JY  et al. DJ-1 induces thioredoxin 1 expression through the Nrf2 pathway. Hum Mol Genet 21:3013-24 (2012). PubMed: 22492997
    • Kim AD  et al. Increased glutathione synthesis following nrf2 activation by vanadyl sulfate in human chang liver cells. Int J Mol Sci 12:8878-94 (2011). WB ; Human . PubMed: 22272109
    • Murakami G  et al. Chemical library screening identifies a small molecule that downregulates SOD1 transcription for drugs to treat amyotrophic lateral sclerosis. J Biomol Screen 16:405-14 (2011). WB . PubMed: 21364087
    • Niture SK  et al. Antioxidant-induced modification of INrf2 cysteine 151 and PKC-delta-mediated phosphorylation of Nrf2 serine 40 are both required for stabilization and nuclear translocation of Nrf2 and increased drug resistance. J Cell Sci 122:4452-64 (2009). WB ; Mouse . PubMed: 19920073

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    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Nrf2 (phospho S40) antibody [EP1809Y] - BSA and Azide free

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Tonsil)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH 9
    Permeabilization
    Yes - Tween, Triton
    Specification
    Tonsil
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    提交于 Jan 12 2022

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