Anti-NRF1抗体[2F9] (ab55744)

Below please find a new discount code and new expiration date regarding testing of ab55744 in zebrafish. Please let me know if you have any questions.

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I am very pleased to hear you would like to accept our offer and test ab55744 in zebrafish. This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for zebrafish and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

DISCOUNT CODE: ***
Expiration date: August 19th, 2012

I am very pleased to hear you would like to accept our offer and test ab55744 in zebrafish. This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for zebrafish and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

Thank you for getting back to me and for providing some further details. It is very much appreciated. It may be worth running human heart or skeletal muscle lysate alongside the samples to see if endogenously expressed NRF1 protein runs differently under the same conditions. Nevertheless, if your customer would prefer to get a replacement vial or a credit note please do let me know. I look forward to hearing from you and hope to solve this problem as soon as possible.

Thank you for contacting Abcam Technical Team and for taking the time to provide some useful details of the experiments. I am very sorry to hear that your customer is having problems with this product. Though you have kindly provided some details, it would be much appreciated if I could get some more information which would help me identify the source of the problem. 1) Image: a) Could you please specify what size are the non-specific bands? Are they lower or higher than the expected size? b) Could you attach an image (saved as a jpeg file) to your e-mail so that I can have a look at the bands. c) There are at least two isoforms have been described in the literature. Could you please comment on which isoform(s) is expected to be expressed in the samples? 2) Construct: Epitope tagging could interact with the recognition of the epitope(s). a) Where is the location of the tag within the reading frame of the NRF1 protein? b) How many copies of tags have been inserted? c) Has the transfection efficiency been tested? 3) Positive control: Since the target protein is tagged, it would be worth running a good positive control alongside with the samples on the same gel to see if the band pattern is the same or not. As the on-line datasheet indicates, human kidney lysates were used to test this antibody, so this type of lysate or human heart could be used as positive control. Thank you for your understanding and co-operation in this matter. I look forward to hearing from you and hope to solve this problem as soon as possible.