Anti-NRAS抗体(ab77392)
Key features and details
- Goat polyclonal to NRAS
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-NRAS抗体
参阅全部 NRAS 一抗 -
描述
山羊多克隆抗体to NRAS -
宿主
Goat -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide within Human NRAS aa 150 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. Accession Number: NP_002515.1
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阳性对照
- WB: MCF7 cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab77392 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunising peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab77392于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (6) |
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
1 hour primary incubation at room temperature is recommended for this product. |
| 说明 |
|---|
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WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa). 1 hour primary incubation at room temperature is recommended for this product. |
靶标
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功能
Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. -
疾病相关
Defects in NRAS are a cause of juvenile myelomonocytic leukemia (JMML) [MIM:607785]. JMML is a pediatric myelodysplastic syndrome that constitutes approximately 30% of childhood cases of myelodysplastic syndrome (MDS) and 2% of leukemia.
Defects in NRAS are the cause of Noonan syndrome type 6 (NS6) [MIM:613224]. A syndrome characterized by facial dysmorphic features such as hypertelorism, a downward eyeslant and low-set posteriorly rotated ears. Other features can include short stature, a short neck with webbing or redundancy of skin, cardiac anomalies, deafness, motor delay and variable intellectual deficits. -
序列相似性
Belongs to the small GTPase superfamily. Ras family. -
翻译后修饰
Palmitoylated by the ZDHHC9-GOLGA7 complex. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi. -
细胞定位
Cell membrane. Golgi apparatus membrane. Shuttles between the plasma membrane and the Golgi apparatus. - Information by UniProt
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数据库链接
- Entrez Gene: 4893 Human
- Entrez Gene: 18176 Mouse
- Entrez Gene: 24605 Rat
- Omim: 164790 Human
- SwissProt: P01111 Human
- SwissProt: P08556 Mouse
- SwissProt: Q04970 Rat
- Unigene: 486502 Human
see all -
别名
- ALPS4 antibody
- AV095280 antibody
- GTPase NRas antibody
see all
图片
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Western blot - Anti-NRAS antibody (ab77392)Anti-NRAS antibody (ab77392) at 0.5 µg/ml + MCF7 (human breast adenocarcinoma cell line) whole cell lysate in RIPA buffer. at 35 µg
Predicted band size: 21 kDa
Observed band size: 21 kDaPrimary incubation for 1 hour at room temperature. Detected by chemiluminescence.
数据表及文件
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SDS download
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Datasheet download
文献 (13)
ab77392 被引用在 13 文献中.
- Yan Y et al. NRAS expression is associated with prognosis and tumor immune microenvironment in lung adenocarcinoma. J Cancer Res Clin Oncol 148:565-575 (2022). PubMed: 34746975
- Ma Y et al. Neuronal miR-29a protects from obesity in adult mice. Mol Metab 61:101507 (2022). PubMed: 35490865
- Nam HJ et al. GPR110 promotes progression and metastasis of triple-negative breast cancer. Cell Death Discov 8:271 (2022). PubMed: 35614051
- Gopal P et al. The Mutational Landscape of Cancer's Vulnerability to Ionizing Radiation. Clin Cancer Res 28:5343-5358 (2022). PubMed: 36222846
- Bery N et al. A potent KRAS macromolecule degrader specifically targeting tumours with mutant KRAS. Nat Commun 11:3233 (2020). PubMed: 32591521