Anti-NRAP抗体(ab122427)
Key features and details
- Rabbit polyclonal to NRAP
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-NRAP抗体 -
描述
兔多克隆抗体to NRAP -
宿主
Rabbit -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
internal sequence amino acids 1309-1393 (RHDFVKERGK LIGPQSVRDD PRIQHCRRMG QLQSELQYRR GATSSQAQFH LPMDMVHLVH AKNAQALASD HDYRTQYHKF TALPE) of the Human NRAP protein (UniProt ID: Q86VF7).
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阳性对照
- IHC-P: Human skeletal muscle and heart muscle tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab122427于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
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IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
May be involved in anchoring the terminal actin filaments in the myofibril to the membrane and in transmitting tension from the myofibrils to the extracellular matrix. -
组织特异性
Expressed in cardiac and skeletal muscle. -
序列相似性
Contains 1 LIM zinc-binding domain.
Contains 44 nebulin repeats. -
细胞定位
Localized at the myotendinous junction in skeletal muscle and at the intercalated disk in cardiac muscle. - Information by UniProt
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数据库链接
- Entrez Gene: 4892 Human
- Omim: 602873 Human
- SwissProt: Q86VF7 Human
- Unigene: 268788 Human
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别名
- N-RAP antibody
- Nebulin-related-anchoring protein antibody
- Nrap antibody
- NRAP_HUMAN antibody
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NRAP antibody (ab122427)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue labelling NRAP with ab122427 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NRAP antibody (ab122427)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human heart muscle tissue labelling NRAP with ab122427 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NRAP antibody (ab122427)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling NRAP with ab122427 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NRAP antibody (ab122427)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue labelling NRAP with ab122427 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab122427 被引用在 2 文献中.
- Li F et al. Expressing a Z-disk nebulin fragment in nebulin-deficient mouse muscle: effects on muscle structure and function. Skelet Muscle 10:2 (2020). PubMed: 31992366
- Truszkowska GT et al. Homozygous truncating mutation in NRAP gene identified by whole exome sequencing in a patient with dilated cardiomyopathy. Sci Rep 7:3362 (2017). WB ; Human . PubMed: 28611399