Anti-NR5A2 / LRH1抗体(ab223211)
Key features and details
- Goat polyclonal to NR5A2 / LRH1
- Suitable for: WB
- Reacts with: Human, Pig
- Isotype: IgG
概述
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产品名称
Anti-NR5A2 / LRH1抗体
参阅全部 NR5A2 / LRH1 一抗 -
描述
山羊多克隆抗体to NR5A2 / LRH1 -
宿主
Goat -
特异性
ab223211 is expected to recognize both reported isoforms (NP_995582.1; NP_003813.1). -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human, Pig
预测可用于: Mouse, Rat, Chicken
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免疫原
Synthetic peptide corresponding to Human NR5A2/ LRH1 aa 200-300 (internal sequence) (Cysteine residue). (NP_995582.1).
Database link: O00482 -
阳性对照
- WB: HepG2 nuclear lysate; Pig ovary lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab223211 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab223211于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 61 kDa).
1 hour primary incubation is recommended for this product. |
| 说明 |
|---|
|
WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 61 kDa). 1 hour primary incubation is recommended for this product. |
靶标
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功能
Binds to the sequence element 5'-AACGACCGACCTTGAG-3' of the enhancer II of hepatitis B virus genes, a critical cis-element of their expression and regulation. May be responsible for the liver-specific activity of enhancer II, probably in combination with other hepatocyte transcription factors. Key regulator of cholesterol 7-alpha-hydroxylase gene (CYP7A) expression in liver. May also contribute to the regulation of pancreas-specific genes and play important roles in embryonic development. -
组织特异性
Abundantly expressed in pancreas, less in liver, very low levels in heart and lung. Expressed in the Hep-G2 cell line. Isoform 1 and isoform 2 seem to be present in fetal and adult liver and Hep-G2 cells. -
序列相似性
Belongs to the nuclear hormone receptor family. NR5 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 395961 Chicken
- Entrez Gene: 2494 Human
- Entrez Gene: 26424 Mouse
- Entrez Gene: 100523688 Pig
- Entrez Gene: 60349 Rat
- Omim: 604453 Human
- SwissProt: O42101 Chicken
- SwissProt: O00482 Human
see all -
别名
- Alpha-1-fetoprotein transcription factor antibody
- B1-binding factor antibody
- B1F antibody
see all
图片
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Western blot - Anti-NR5A2 / LRH1 antibody (ab223211)Anti-NR5A2 / LRH1 antibody (ab223211) at 1 µg/ml + HepG2 (human liver hepatocellular carcinoma cell line) nuclear lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 61 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour.
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Western blot - Anti-NR5A2 / LRH1 antibody (ab223211)Anti-NR5A2 / LRH1 antibody (ab223211) at 0.3 µg/ml + Pig ovary lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 61 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour.
数据表及文件
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Datasheet download
文献 (1)
ab223211 被引用在 1 文献中.
- Sun W et al. LRH1 Promotes Tumor Cell Proliferation and Migration and Is Correlated With Poor Prognosis in Ovarian Cancer. Front Oncol 10:583566 (2020). PubMed: 33194722
