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重组RabMAb

重组Anti-NR2F6/EAR-2抗体[EPR22260] (ab223265)

Submit a review Submit a question References (1)
Western blot - Anti-NR2F6/EAR-2 antibody [EPR22260] (ab223265)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR2F6/EAR-2 antibody [EPR22260] (ab223265)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR2F6/EAR-2 antibody [EPR22260] (ab223265)
  • Immunoprecipitation - Anti-NR2F6/EAR-2 antibody [EPR22260] (ab223265)
  • Flow Cytometry (Intracellular) - Anti-NR2F6/EAR-2 antibody [EPR22260] (ab223265)
  • Anti-NR2F6/EAR-2 antibody [EPR22260] (ab223265)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22260] to NR2F6/EAR-2
  • Suitable for: Flow Cyt (Intra), IP, WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-NR2F6/EAR-2抗体[EPR22260]
    参阅全部 NR2F6/EAR-2 一抗

  • 描述

    兔单克隆抗体[EPR22260] to NR2F6/EAR-2

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Flow Cyt (Intra), IP, WB, IHC-Pmore details
    不适用于: ICC/IF

  • 种属反应性

    与反应: Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: HeLa, HCT116, HT-29, HEK-293T and HepG2 whole cell lysates. IHC-P: Human kidney and human breast carcinoma tissues. Flow Cyt (intra): HeLa cells. IP: HeLa whole cell lysate.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • 存储溶液

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    EPR22260

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab223265于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
1/500.
IP
1/30.
WB
1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
说明
Flow Cyt (Intra)
1/500.
IP
1/30.
WB
1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
应用说明
Is unsuitable for ICC/IF.

靶标

  • 功能

    Transcription factor predominantly involved in transcriptional repression. Binds to promoter/enhancer response elements that contain the imperfect 5'-AGGTCA-3' direct or inverted repeats with various spacings which are also recognized by other nuclear hormone receptors. Involved in modulation of hormonal responses. Represses transcriptional activity of the lutropin-choriogonadotropic hormone receptor/LHCGR gene, the renin/REN gene and the oxytocin-neurophysin/OXT gene. Represses the triiodothyronine-dependent and -independent transcriptional activity of the thyroid hormone receptor gene in a cell type-specific manner. The corepressing function towards thyroid hormone receptor beta/THRB involves at least in part the inhibition of THRB binding to triiodothyronine response elements (TREs) by NR2F6. Inhibits NFATC transcription factor DNA binding and subsequently its transcriptional activity. Acts as transcriptional repressor of IL-17 expression in Th-17 differentiated CD4(+) T cells and may be involved in induction and/or maintenance of peripheral immunological tolerance and autoimmunity. Involved in development of forebrain circadian clock; is required early in the development of the locus coeruleus (LC).

  • 组织特异性

    Expressed in heart, placenta, liver, skeletal muscle, kidney and pancreas.

  • 序列相似性

    Belongs to the nuclear hormone receptor family. NR2 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.

  • 细胞定位

    Nucleus.
  • Information by UniProt

  • 数据库链接

    • 别名

      • EAR 2 antibody
      • EAR-2 antibody
      • EAR2 antibody
      • ERBA RELATED 2 antibody
      • ERBA related gene 2 antibody
      • ERBAL2 antibody
      • Nr2f6 antibody
      • NR2F6_HUMAN antibody
      • Nuclear receptor subfamily 2 group F member 6 antibody
      • Orphan nuclear receptor EAR2 (V erbA related protein EAR 2) antibody
      • v erb a avian erythroblastic leukemia viral oncogene homolog like antibody
      • V erbA related protein EAR 2 antibody
      • V-erbA-related protein 2 antibody
      see all

    图片

    • Western blot - Anti-NR2F6/EAR-2 antibody [EPR22260] (ab223265)
      Western blot - Anti-NR2F6/EAR-2 antibody [EPR22260] (ab223265)
      All lanes : Anti-NR2F6/EAR-2 antibody [EPR22260] (ab223265) at 1/1000 dilution

      Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma), whole cell lysate
      Lane 2 : HCT 116 (human colorectal carcinoma cell line), whole cell lysate
      Lane 3 : HT-29 (human colorectal adenocarcinoma cell line), whole cell lysate
      Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen), whole cell lysate
      Lane 5 : HepG2 (human liver hepatocellular carcinoma cell line), whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 43 kDa
      Observed band size: 43 kDa


      Exposure time: 3 minutes


      Blocking/Diluting buffer and concentration: 5% NFDM/TBST 

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR2F6/EAR-2 antibody [EPR22260] (ab223265)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR2F6/EAR-2 antibody [EPR22260] (ab223265)

      Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling NR2F6/EAR-2 with ab223265 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on human breast carcinoma (PMID: 27775588). Counterstained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR2F6/EAR-2 antibody [EPR22260] (ab223265)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR2F6/EAR-2 antibody [EPR22260] (ab223265)

      Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling NR2F6/EAR-2 with ab223265 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on human kidney (PMID: 10713182). Counterstained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

       

    • Immunoprecipitation - Anti-NR2F6/EAR-2 antibody [EPR22260] (ab223265)
      Immunoprecipitation - Anti-NR2F6/EAR-2 antibody [EPR22260] (ab223265)

      NR2F6/EAR-2 was immunoprecipitated from 0.35 mg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab223265 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab223265 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.

      Lane 1: HeLa whole cell lysate 10 µg (input).
      Lane 2: ab223265 IP in HeLa whole cell lysate.
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab223265 in HeLa whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.
      Exposure time: 30 seconds.

    • Flow Cytometry (Intracellular) - Anti-NR2F6/EAR-2 antibody [EPR22260] (ab223265)
      Flow Cytometry (Intracellular) - Anti-NR2F6/EAR-2 antibody [EPR22260] (ab223265)

      Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed 90% methanol permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling NR2F6/EAR-2 with ab223265 at 1/40 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody. 

       

       

    • Anti-NR2F6/EAR-2 antibody [EPR22260] (ab223265)
      Anti-NR2F6/EAR-2 antibody [EPR22260] (ab223265)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (1)

    发表研究结果有使用 ab223265？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab223265 被引用在 1 文献中.

    • Yang SL  et al. The expression and biological effect of NR2F6 in non-small cell lung cancer. Front Oncol 12:940234 (2022). PubMed: 36119482

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