AB109179

重组Anti-NR2E1/Tailless/TLX抗体[EPR4491]

Anti-NR2E1/Tailless/TLX antibody [EPR4491]

RabMAb
Recombinant
了解详情

(1 Review)

(4 Publications)

Rabbit Recombinant Monoclonal NR2E1/Tailless/TLX antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 4 publications.

查看别名

TLX, NR2E1, Nuclear receptor subfamily 2 group E member 1, Nuclear receptor TLX, Protein tailless homolog, Tll, hTll

2 Images

Unknown

Western blot - Anti-NR2E1/Tailless/TLX antibody [EPR4491] (AB109179)

All lanes:

Western blot - Anti-NR2E1/Tailless/TLX antibody [EPR4491] (ab109179) at 1/1000 dilution

Lane 1:

Fetal brain lysate at 10 µg

Lane 2:

Human hippocampus lysate at 10 µg

Lane 3:

SH-SY5Y lysate at 10 µg

Lane 4:

Jurkat lysate at 10 µg

Predicted band size: 43 kDa

false

OI-RD Scanning - Anti-NR2E1/Tailless/TLX antibody [EPR4491] (AB109179)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-NR2E1/Tailless/TLX antibody [EPR4491] (AB109179)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR4491

亚型

IgG

不含载体蛋白

反应种属

Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

运输条件

Blue Ice

储存信息

Stable for 12 months at -20°C

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

NR2E1 also known as Tailless or TLX is a nuclear receptor protein with a mass of approximately 50 kDa. It acts as a transcription factor functioning primarily by binding to specific DNA sequences and regulating gene expression. NR2E1 is highly expressed in the brain particularly in areas involved in regulating neurogenesis and maintaining neural stem cells. Additionally it shows expression in the retina and various embryonic tissues where it plays an important role in development.

Biological function summary

NR2E1 influences the development and function of the central nervous system by regulating neural stem cell proliferation and differentiation. It forms a transcriptional complex by interacting with co-repressors leading to suppression of target gene expression. This protein helps to maintain neural precursor populations and controls the balance between stem cell self-renewal and differentiation an important process in neurodevelopment and adult brain function.

Pathways

The NR2E1 protein participates in the Notch signaling pathway and is also associated with the Wnt signaling pathway. These pathways are important for cellular differentiation and proliferation in the context of neurodevelopment. NR2E1 interacts with several key proteins including PAX6 and SOX2 which are important in the regulation and identity of neural precursor cells ensuring proper brain development and function through these pathways.

NR2E1 links to neurodegenerative diseases and psychiatric disorders. Abnormalities in its expression or function are associated with conditions like schizophrenia and depression. Its role in maintaining neural stem cell populations implicates it in disorders involving neurogenesis dysregulation. Additionally NR2E1's interactions with proteins such as REST may have implications in understanding and potentially addressing these disorders as REST is involved in neuronal gene silencing in various neuropathological conditions.

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

Orphan receptor that binds DNA as a monomer to hormone response elements (HRE) containing an extended core motif half-site sequence 5'-AAGGTCA-3' in which the 5' flanking nucleotides participate in determining receptor specificity (By similarity). May be required to pattern anterior brain differentiation. Involved in the regulation of retinal development and essential for vision. During retinogenesis, regulates PTEN-Cyclin D expression via binding to the promoter region of PTEN and suppressing its activity (By similarity). May be involved in retinoic acid receptor (RAR) regulation in retinal cells.

See full target information NR2E1

style

left-column

文献 (4)

Recent publications for all applications. Explore the full list and refine your search

EMBO reports 25:704-724 PubMed38263327

2024

Mitochondrial translocation of TFEB regulates complex I and inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Chiara Calabrese,Hendrik Nolte,Melissa R Pitman,Raja Ganesan,Philipp Lampe,Raymond Laboy,Roberto Ripa,Julia Fischer,Ruhi Polara,Sameer Kumar Panda,Sandhya Chipurupalli,Saray Gutierrez,Daniel Thomas,Stuart M Pitson,Adam Antebi,Nirmal Robinson

iScience 25:105050 PubMed36185374

2022

Induced retinal pigment epithelial cells with anti-epithelial-to-mesenchymal transition ability delay retinal degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Haibin Tian,Zhiyang Chen,Xiaoman Zhu,Qingjian Ou,Zhe Wang,Binxin Wu,Jing-Ying Xu,Caixia Jin,Furong Gao,Juan Wang,Jingfa Zhang,Jieping Zhang,Lixia Lu,Guo-Tong Xu

Biochimica et biophysica acta. Molecular basis of disease 1868:166515 PubMed35932893

2022

The nuclear receptor TLX (NR2E1) inhibits growth and progression of triple- negative breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Adam T Nelczyk,Liqian Ma,Anasuya Das Gupta,Hashni Epa Vidana Gamage,Michael T McHenry,Madeline A Henn,Mohammed Kadiri,Yu Wang,Natalia Krawczynska,Shruti Bendre,Sisi He,Sayyed Hamed Shahoei,Zeynep Madak-Erdogan,Shih-Hsuan Hsiao,Tareq Saleh,Valerie Carpenter,David A Gewirtz,Michael J Spinella,Erik R Nelson

Molecules (Basel, Switzerland) 23: PubMed30441799

2018

Computer-Aided Discovery of Small Molecule Inhibitors of Transcriptional Activity of TLX (NR2E1) Nuclear Receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Evgenia Dueva,Kriti Singh,Anastasia Kalyta,Eric LeBlanc,Paul S Rennie,Artem Cherkasov

View all publications

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-NR2E1/Tailless/TLX抗体[EPR4491]

Anti-NR2E1/Tailless/TLX antibody [EPR4491]

Western blot - Anti-NR2E1/Tailless/TLX antibody [EPR4491] (AB109179)

OI-RD Scanning - Anti-NR2E1/Tailless/TLX antibody [EPR4491] (AB109179)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原