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AB181049

重组Anti-NQO2抗体[EPR13345]

Anti-NQO2 antibody [EPR13345]

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(3 Publications)

Rabbit Recombinant Monoclonal NQO2 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Mouse, Rat samples. Cited in 3 publications.

查看别名

NMOR2, NQO2, Ribosyldihydronicotinamide dehydrogenase [quinone], NRH dehydrogenase [quinone] 2, NRH:quinone oxidoreductase 2, Quinone reductase 2, QR2

3 Images
Flow Cytometry (Intracellular) - Anti-NQO2 antibody [EPR13345] (AB181049)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-NQO2 antibody [EPR13345] (AB181049)

Intracellular flow cytometric analysis of C6 cells labeling NQO2 with ab181049 at 1/140 dilution (red). Goat anti rabbit IgG (FITC) used as the secondary at 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde.

Western blot - Anti-NQO2 antibody [EPR13345] (AB181049)
  • WB

Supplier Data

Western blot - Anti-NQO2 antibody [EPR13345] (AB181049)

Blocking/ Dilution buffer : 5% NFDM /TBST.

All lanes:

Western blot - Anti-NQO2 antibody [EPR13345] (ab181049) at 1/5000 dilution

Lane 1:

L6 cell lysate at 10 µg

Lane 2:

C2C12 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 26 kDa

false

Western blot - Anti-NQO2 antibody [EPR13345] (AB181049)
  • WB

Supplier Data

Western blot - Anti-NQO2 antibody [EPR13345] (AB181049)

All lanes:

Western blot - Anti-NQO2 antibody [EPR13345] (ab181049) at 1/1000 dilution

Lane 1:

Mouse kidney tissue lysate at 20 µg

Lane 2:

Rat heart tissue lysate at 20 µg

Lane 3:

Rat kidney tissue lysate at 20 µg

Lane 4:

Rat spleen tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 26 kDa

false

不同偶联物与剂型 (1)

  • Carrier free

    Anti-NQO2 antibody [EPR13345] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR13345

亚型

IgG

不含载体蛋白

No

反应种属

Mouse, Rat

应用

Flow Cyt (Intra), WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/140", "FlowCytIntra-species-notes": "<p><a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }
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产品详情

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式
Liquid
纯度
Tissue culture supernatant
存储溶液
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

NQO2 also known as NRH:quinone oxidoreductase 2 is a flavoprotein enzyme playing roles in cellular redox reactions. It has a molecular mass of approximately 25 kDa. NQO2 is expressed widely in human tissues such as liver colon and brain. It catalyzes the two-electron reduction of quinones to hydroquinones therefore helping prevent oxidative stress by converting reactive quinone species into less reactive and more stable molecules. The protein often interacts with other cellular components to perform its functions effectively.
Biological function summary

NQO2 participates in detoxification processes and acts as a dihydronicotinamide riboside:quinone oxidoreductase. Although it is not part of a large protein complex NQO2 may associate with other molecules or enzymes that modulate cellular antioxidant defense systems. It serves as a backup system when other primary antioxidant enzymes such as NQO1 are impaired. This function reflects the importance of NQO2 in maintaining cellular health and response to environmental stresses.

Pathways

NQO2 is involved in metabolism and oxidative stress response pathways. Its role in quinone detoxification connects it to the cellular defense mechanism against oxidative damage interacting with proteins like NQO1. NQO2's enzymatic activity goes hand-in-hand with the NAD(P)H dehydrogenase (quinone) pathway which collaborates with other proteins to maintain redox homeostasis and protect cells against electrophilic and oxidative stress.

Alterations in NQO2 expression or function are linked to cancer and neurodegenerative diseases. NQO2's involvement in detoxifying potent quinones links it to cancerous developments when these processes fail while its role against oxidative stress connects it to neurodegenerative conditions like Parkinson’s disease. During these conditions interactions with proteins such as NQO1 and p53 can be significant. The balance of NQO2 activity may contribute to disease progression or suppression highlighting its therapeutic potential in disease management.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinones involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.
See full target information NQO2
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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 23:920 PubMed40817243

2025

MRO/HNRNPU/CCL5 feedback loop amplifies M2 macrophage and breast cancer cell crosstalk to drive progression.

Applications

Unspecified application

Species

Unspecified reactive species

Zhirui Zeng,Xin Bao,Shan Lei,Wenjing Tang,Huading Tai,Lu Wang,Dahuan Li,Shu Liu,Tengxiang Chen

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2400253 PubMed39119847

2024

Homeostatic Shrinkage of Dendritic Spines Requires Melatonin Type 3 Receptor Activation During Sleep.

Applications

Unspecified application

Species

Unspecified reactive species

Shiyin Li,Xin Li,Minmin Lu,Quanhui Chen,Di Yao,Xiaoqian Yu,Zhen Li,Woo-Ping Ge,Na Wang,Jiehua Jin,Yaling Wang,Yixiang Liao,Fenlan Luo,Jie Yan,Xuedan Chen,Chenggang Jiang,Faguo Yue,Dong Gao,Xiangdong Tang,Hong Guo,Yanjiang Wang,Xiaowei Chen,Jianxia Xia,Min Xu,Shuancheng Ren,Chao He,Zhian Hu

Journal of proteome research 20:2596-2606 PubMed33793246

2021

Reduced Nicotinamide Mononucleotide (NMNH) Potently Enhances NAD and Suppresses Glycolysis, the TCA Cycle, and Cell Growth.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Liu,Chengting Luo,Ting Li,Wenhao Zhang,Zhaoyun Zong,Xiaohui Liu,Haiteng Deng
View all publications
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替代产品

Primary Antibodies

AB236917

Anti-NQO2 antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NQO2 antibody (AB236917)

  • 0
  • 0
Primary Antibodies

AB137612

Anti-NQO2 antibody

Western blot - Anti-NQO2 antibody (AB137612)

  • 0
  • 0

搭配使用产品

Proteins & Peptides

AB93933

Recombinant Human NQO2 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human NQO2 protein (His tag N-Terminus) (AB93933)

  • 0
  • 0
Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 16
Primary Antibodies

AB80588

Anti-NQO1 antibody [EPR3309]

20ul selling size|RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-NQO1 antibody [EPR3309] (AB80588)

  • 4
  • 5
Primary Antibodies

AB206129

Anti-PKM antibody [EPR10138(B)] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-PKM antibody [EPR10138(B)] - BSA and Azide free (AB206129)

  • 5
  • 2

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