重组Anti-NQO1抗体[EPR3309] (ab80588)
![Western blot - Anti-NQO1 antibody [EPR3309] (ab80588)](https://www.abcam.cn/ps/products/80/ab80588/Images/ab80588-297796-ab80588KOWBJRIZBranford.jpg "Western blot - Anti-NQO1 antibody [EPR3309] (ab80588)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3309] to NQO1
- Suitable for: Flow Cyt (Intra), WB, IP, ICC/IF
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-NQO1抗体[EPR3309]
参阅全部 NQO1 一抗 -
描述
兔单克隆抗体[EPR3309] to NQO1 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, IP, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human NQO1 (N terminal). The exact sequence is proprietary.
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阳性对照
- WB: SH-SY5Y, MCF7, HeLa and A549 cell lysates, rat brain tissue lysate. ICC/IF: MCF-7 cells. IP: HeLa cell lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR3309 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab80588于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
Use at an assay dependent concentration.
|
|
| WB | (4) |
1/10000 - 1/150000. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).
|
| IP |
1/30 - 1/50.
|
|
| ICC/IF |
1/50 - 1/80.
|
| 说明 |
|---|
|
Flow Cyt (Intra)
Use at an assay dependent concentration. |
|
WB
1/10000 - 1/150000. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa). |
|
IP
1/30 - 1/50. |
|
ICC/IF
1/50 - 1/80. |
靶标
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功能
The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. -
序列相似性
Belongs to the NAD(P)H dehydrogenase (quinone) family. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 1728 Human
- Entrez Gene: 18104 Mouse
- Entrez Gene: 24314 Rat
- Omim: 125860 Human
- SwissProt: P15559 Human
- SwissProt: Q64669 Mouse
- SwissProt: P05982 Rat
- Unigene: 406515 Human
see all -
别名
- Azoreductase antibody
- Cytochrome b 5 reductase antibody
- DHQU antibody
see all
图片
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Western blot - Anti-NQO1 antibody [EPR3309] (ab80588)All lanes : Anti-NQO1 antibody [EPR3309] (ab80588) at 1 µg/ml
Lane 1 : Wild-type HAP1 whole cell lysate at 40 µg
Lane 2 : NQO1 knockout HAP1 whole cell lysate at 40 µg
Lane 3 : HepG2 whole cell lysate at 20 µg
Lane 4 : MEF1 whole cell lysate at 20 µg
Lane 5 : Human Kidney tissue lysate at 20 µg
Lane 6 : Mouse Kidney tissue lysate at 20 µg
Predicted band size: 31 kDaLanes 1 - 6: Merged signal (red and green). Green - ab80588 observed at 31 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab80588 was shown to recognize NQO1 in wild-type HAP1 cells as signal was lost at the expected MW in NQO1 knockout cells. Wild-type and NQO1 knockout samples were subjected to SDS-PAGE. Ab80588 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-NQO1 antibody [EPR3309] (ab80588)](https://www.abcam.cn/ps/products/80/ab80588/Images/ab80588-239097-ab80588pwb.jpg)
Western blot - Anti-NQO1 antibody [EPR3309] (ab80588)All lanes : Anti-NQO1 antibody [EPR3309] (ab80588) at 1/25000 dilution (purified)
Lane 1 : SH-SY5Y cell lysate
Lane 2 : Rat brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 31 kDa
Observed band size: 31 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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![Immunocytochemistry/ Immunofluorescence - Anti-NQO1 antibody [EPR3309] (ab80588)](https://www.abcam.cn/ps/products/80/ab80588/Images/ab80588-239101-ab80588piccif.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-NQO1 antibody [EPR3309] (ab80588)Immunocytochemsitry/Immunofluorescence analysis of MCF-7 cells labelling NQO1 (green) with purified ab80588 at 1/80. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI (blue).
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![Flow Cytometry (Intracellular) - Anti-NQO1 antibody [EPR3309] (ab80588)](https://www.abcam.cn/ps/products/80/ab80588/Images/ab80588-269571-ab80588FC1.jpg)
Flow Cytometry (Intracellular) - Anti-NQO1 antibody [EPR3309] (ab80588)ab80588 staining NQO1in the human cell line MCF-7 (human breast carcinoma) by intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde, permeabilized with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/20. A goat anti rabbit IgG (Alexa Fluorr® 488) at a dilution of 1/2000 was used as the secondary antibody.
Isoytype control: Rabbit monoclonal IgG (Black)
Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)
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![Immunoprecipitation - Anti-NQO1 antibody [EPR3309] (ab80588)](https://www.abcam.cn/ps/products/80/ab80588/Images/ab80588-239099-ab80588pip.jpg)
Immunoprecipitation - Anti-NQO1 antibody [EPR3309] (ab80588)ab80588 (purified) at 1/50 immunoprecipitating NQO1 in HeLa cell lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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![Western blot - Anti-NQO1 antibody [EPR3309] (ab80588)](https://www.abcam.cn/ps/products/80/ab80588/Images/NQO1-Primary-antibodies-ab80588-1.jpg)
Western blot - Anti-NQO1 antibody [EPR3309] (ab80588)All lanes : Anti-NQO1 antibody [EPR3309] (ab80588) at 1/50000 dilution (unpurified)
Lane 1 : MCF7
cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : A549
cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 31 kDa
Observed band size: 31 kDa -
![Anti-NQO1 antibody [EPR3309] (ab80588)](https://www.abcam.cn/ps/products/80/ab80588/Images/ab80588-11-benefits-of-recombinant-antibodies.png)
Anti-NQO1 antibody [EPR3309] (ab80588)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (135)
ab80588 被引用在 135 文献中.
- Xu WN et al. Sesn2 Serves as a Regulator between Mitochondrial Unfolded Protein Response and Mitophagy in Intervertebral Disc Degeneration. Int J Biol Sci 19:571-592 (2023). PubMed: 36632468
- Bhat A et al. Quantitative Proteomics Identifies Novel Nrf2-Mediated Adaptative Signaling Pathways in Skeletal Muscle Following Exercise Training. Antioxidants (Basel) 12:N/A (2023). PubMed: 36671013
- Zhao F et al. A Multifunctional (-)-Meptazinol-Serotonin Hybrid Ameliorates Oxidative Stress-Associated Apoptotic Neuronal Death and Memory Deficits via Activating the Nrf2/Antioxidant Enzyme Pathway. Oxid Med Cell Longev 2023:6935947 (2023). PubMed: 36819782
- Li Y et al. Human umbilical cord-derived mesenchymal stem cell transplantation supplemented with curcumin improves the outcomes of ischemic stroke via AKT/GSK-3β/β-TrCP/Nrf2 axis. J Neuroinflammation 20:49 (2023). PubMed: 36829224
- Xu X et al. Coumarin-derived imino sulfonate 5h ameliorates cardiac injury induced by myocardial infarction via activating the Sirt1/Nrf2 signaling pathway. Eur J Pharmacol 945:175615 (2023). PubMed: 36841283