重组Anti-NPY5R抗体[EPR8382] (ab133757)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8382] to NPY5R
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-NPY5R抗体[EPR8382]
参阅全部 NPY5R 一抗 -
描述
兔单克隆抗体[EPR8382] to NPY5R -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, ICC/IFmore details
不适用于: IHC-P -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide within Human NPY5R aa 1-100 (extracellular). The exact sequence is proprietary.
Database link: Q15761 -
阳性对照
- Human hypothalamus lysate, Human fetal brain lysate, Ramos cell lysate, U87 MG cells and cell lysate, Caco2 cell lysate, BxPC 3 cells and cell lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 1.80 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR8382 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133757于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/1000 - 1/10000.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB | (1) |
1/10000 - 1/50000. Detects a band of approximately 52 kDa (predicted molecular weight: 51 kDa).
|
ICC/IF |
1/250 - 1/500.
|
说明 |
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Flow Cyt (Intra)
1/1000 - 1/10000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/10000 - 1/50000. Detects a band of approximately 52 kDa (predicted molecular weight: 51 kDa). |
ICC/IF
1/250 - 1/500. |
靶标
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功能
Receptor for neuropeptide Y and peptide YY. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity. Seems to be associated with food intake. Could be involved in feeding disorders. -
组织特异性
Brain; hypothalamus. -
序列相似性
Belongs to the G-protein coupled receptor 1 family. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 4889 Human
- Entrez Gene: 18168 Mouse
- Entrez Gene: 25340 Rat
- Omim: 602001 Human
- SwissProt: Q15761 Human
- SwissProt: O70342 Mouse
- SwissProt: Q63634 Rat
- Unigene: 519058 Human
see all -
别名
- Neuropeptide Y receptor type 5 antibody
- Neuropeptide Y receptor Y5 antibody
- NPY 5R antibody
see all
图片
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All lanes : Anti-NPY5R antibody [EPR8382] (ab133757) at 1/10000 dilution
Lane 1 : Human hypothalamus lysate
Lane 2 : Human fetal brain lysate
Lane 3 : Ramos cell lysate
Lane 4 : U87 MG cell lysate
Lane 5 : Caco 2 cell lysate
Lane 6 : BxPC 3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 51 kDa -
Immunofluorescent staining of BxPC3 cells labelling NPY5R with ab133757 at 1/250.
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Immunofluorescent staining of U87 MG cells labelling NPY5R with ab133757 at 1/250.
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Overlay histogram showing SHSY-5Y cells stained with ab133757 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab133757, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed SHSY-5Y cells used under the same conditions. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab133757 被引用在 7 文献中.
- Pascetta SA et al. Pharmacological inhibition of neuropeptide Y receptors Y1 and Y5 reduces hypoxic breast cancer migration, proliferation, and signaling. BMC Cancer 23:494 (2023). PubMed: 37264315
- Yang T et al. The role of NPY2R/NFATc1/DYRK1A regulatory axis in sebaceous glands for sebum synthesis. Cell Mol Biol Lett 28:60 (2023). PubMed: 37501148
- Ramlan H & Damanhuri HA Attenuation of the Counter-Regulatory Glucose Response in CVLM C1 Neurons: A Possible Explanation for Anorexia of Aging. Biomolecules 12:N/A (2022). PubMed: 35327640
- Lu C et al. Hypoxia-activated neuropeptide Y/Y5 receptor/RhoA pathway triggers chromosomal instability and bone metastasis in Ewing sarcoma. Nat Commun 13:2323 (2022). PubMed: 35484119
- Abualsaud N et al. Neuropeptide Y/Y5 Receptor Pathway Stimulates Neuroblastoma Cell Motility Through RhoA Activation. Front Cell Dev Biol 8:627090 (2020). PubMed: 33681186
- Liao T et al. Liraglutide Lowers Body Weight Set Point in DIO Rats and its Relationship with Hypothalamic Microglia Activation. Obesity (Silver Spring) 28:122-131 (2020). PubMed: 31773909
- Boudjadi S et al. Involvement of the Integrin a1ß1 in the Progression of Colorectal Cancer. Cancers (Basel) 9:N/A (2017). PubMed: 28933766