Anti-NPTN抗体(ab272652)
Key features and details
- Rabbit polyclonal to NPTN
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-NPTN抗体 -
描述
兔多克隆抗体to NPTN -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, IHC-P, WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human NPTN aa 200-350.
Database link: Q9Y639 -
阳性对照
- WB: A-549 cells. IHC-P: Human cerebellum tissue. IF: MCF7 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272652于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
|
|
| IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml.
|
| 说明 |
|---|
|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
|
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
靶标
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功能
Probable homophilic and heterophilic cell adhesion molecule involved in long term potentiation at hippocampal excitatory synapses through activation of p38MAPK. May also regulate neurite outgrowth by activating the FGFR1 signaling pathway. May play a role in synaptic plasticity. -
组织特异性
Isoform 1 is ubiquitously expressed. Isoform 2 is expressed in brain cortex and cerebellum (at protein level). -
序列相似性
Contains 3 Ig-like (immunoglobulin-like) domains. -
结构域
Some isoforms lack the first Ig-like domain which may confer homophilic adhesion activity. However, they can bind and activate FGFR1. -
翻译后修饰
N-glycosylated. -
细胞定位
Cell membrane. Enriched at post-synaptic density. - Information by UniProt
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数据库链接
- Entrez Gene: 27020 Human
- Omim: 612820 Human
- SwissProt: Q9Y639 Human
- Unigene: 713221 Human
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别名
- GP55 antibody
- GP65 antibody
- Neuroplastin antibody
see all
图片
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Western blot - Anti-NPTN antibody (ab272652)Lane 2 : Anti-NPTN antibody (ab272652) at 0.4 µg/ml
Lane 1 : Molecular Weight ladder
Lane 2 : A-549 cells (Human lung carcinoma cell line) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPTN antibody (ab272652)Paraffin-embedded formalin-fixed human cerebellum tissue stained for NPTN using ab272652 at 1/50 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-NPTN antibody (ab272652)PFA fixed, Triton X-100 permeablized MCF7 cells (human breast adenocarcinoma cell line) labeling NPTN (green) using ab272652 at 2 ug/ml in ICC/IF analysis.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab272652 被引用在 1 文献中.
- Jin L et al. ETS1-activated SNHG10 exerts oncogenic functions in glioma via targeting miR-532-3p/FBXL19 axis. Cancer Cell Int 20:589 (2020). PubMed: 33298070