重组Anti-NPR-C抗体[EPR12716] - C-terminal (ab177954)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12716] to NPR-C - C-terminal
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
-
产品名称
Anti-NPR-C抗体[EPR12716] - C-terminal
参阅全部 NPR-C 一抗 -
描述
兔单克隆抗体[EPR12716] to NPR-C - C-terminal -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WBmore details
不适用于: IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- Human fetal kidney, Human placenta, and HeLa lysates; HeLa cells.
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR12716 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
- Anti-NPR-C antibody [EPR12716] - BSA and Azide free (ab250024)
- APC Anti-NPR-C antibody [EPR12716] - C-terminal (ab319282)
- PE Anti-NPR-C antibody [EPR12716] - C-terminal (ab319430)
- Alexa Fluor® 488 Anti-NPR-C antibody [EPR12716] - C-terminal (ab319564)
- Alexa Fluor® 647 Anti-NPR-C antibody [EPR12716] - C-terminal (ab319677)
-
Isotype control
-
Positive Controls
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab177954于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 60 kDa).
|
说明 |
---|
Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 60 kDa). |
靶标
-
功能
Receptor for the natriuretic peptide hormones, binding with similar affinities atrial natriuretic peptide NPPA/ANP, brain natriuretic peptide NPPB/BNP, and C-type natriuretic peptide NPPC/CNP. May function as a clearance receptor for NPPA, NPPB and NPPC, regulating their local concentrations and effects. May regulate diuresis, blood pressure and skeletal development. Does not have guanylate cyclase activity. -
序列相似性
Belongs to the ANF receptor family. -
细胞定位
Membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 4883 Human
- Entrez Gene: 18162 Mouse
- Entrez Gene: 25339 Rat
- Omim: 108962 Human
- SwissProt: P17342 Human
- SwissProt: P70180 Mouse
- SwissProt: P41740 Rat
- Unigene: 237028 Human
see all -
别名
- ANP C antibody
- ANP-C antibody
- ANPC antibody
see all
图片
-
All lanes : Anti-NPR-C antibody [EPR12716] - C-terminal (ab177954) at 1/1000 dilution (purified)
All lanes : Human tissue lysate
Lysates/proteins at 1/10 dilution per lane.
Secondary
All lanes : HRP anti-rabbit, specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 60 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
-
All lanes : Anti-NPR-C antibody [EPR12716] - C-terminal (ab177954) at 1/7000 dilution (purified)
Lane 1 : Mouse kidney tissue lysate
Lane 2 : Rat kidney tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 60 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
-
All lanes : Anti-NPR-C antibody [EPR12716] - C-terminal (ab177954) at 1/1000 dilution (unpurified)
Lane 1 : Mouse kidney tissue lysate
Lane 2 : Rat kidney tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 60 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
-
Overlay histogram showingHeLa cells fixed in 2% PFA and stained with purified ab177954 at a dilution of 1 in 270 (pink line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 150. Rabbit monoclonal IgG was used as an isotype control.
-
All lanes : Anti-NPR-C antibody [EPR12716] - C-terminal (ab177954) at 1/1000 dilution (unpurified)
Lane 1 : Human fetal kidney tissue lysate
Lane 2 : Human placenta lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 60 kDa -
Intracellular flow cytometric analysis of permeabilized HeLa cells labeling NPR-C with unpurifiedab177954 at 1/100 dilution (red) or a rabbit IgG (negative) (green).
数据表及文件
-
SDS download
-
Datasheet download
文献 (4)
ab177954 被引用在 4 文献中.
- Zhang YL et al. Metformin combats high glucose-induced damage to the osteogenic differentiation of human periodontal ligament stem cells via inhibition of the NPR3-mediated MAPK pathway. Stem Cell Res Ther 13:305 (2022). PubMed: 35841070
- Roos J et al. miR-146a regulates insulin sensitivity via NPR3. Cell Mol Life Sci 78:2987-3003 (2021). PubMed: 33206203
- Zhu XL et al. High salt-induced weakness of anti-oxidative function of natriuretic peptide receptor-C and podocyte damage in the kidneys of Dahl rats. Chin Med J (Engl) 133:1182-1191 (2020). PubMed: 32433050
- Ding DZ et al. C-type natriuretic peptide prevents angiotensin II-induced atrial connexin 40 and 43 dysregulation by activating AMP-activated kinase signaling. Mol Med Rep 20:5091-5099 (2019). PubMed: 31638216