AB177954

重组Anti-NPR-C抗体[EPR12716] - C-terminal

Anti-NPR-C antibody [EPR12716] - C-terminal

RabMAb
Recombinant
了解详情

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(5 Publications)

Rabbit Recombinant Monoclonal NPR-C antibody. C-terminal. Suitable for WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 5 publications.

查看别名

ANPRC, C5orf23, NPRC, NPR3, Atrial natriuretic peptide receptor 3, Atrial natriuretic peptide clearance receptor, Atrial natriuretic peptide receptor type C, ANP-C, ANPR-C, NPR-C

6 Images

Flow Cytometry (Intracellular) - Anti-NPR-C antibody [EPR12716] - C-terminal (AB177954)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-NPR-C antibody [EPR12716] - C-terminal (AB177954)

Overlay histogram showingHeLa cells fixed in 2% PFA and stained with purified ab177954 at a dilution of 1 in 270 (pink line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 150. Rabbit monoclonal IgG was used as an isotype control.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-NPR-C antibody [EPR12716] - C-terminal (AB177954)

Intracellular flow cytometric analysis of permeabilized HeLa cells labeling NPR-C with unpurifiedab177954 at 1/100 dilution (red) or a rabbit IgG (negative) (green).

Supplier Data

Western blot - Anti-NPR-C antibody [EPR12716] - C-terminal (AB177954)

All lanes:

Western blot - Anti-NPR-C antibody [EPR12716] - C-terminal (ab177954) at 1/1000 dilution

Lane 1:

Human fetal kidney tissue lysate at 10 µg

Lane 2:

Human placenta lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 60 kDa

false

Lab

Western blot - Anti-NPR-C antibody [EPR12716] - C-terminal (AB177954)

Blocking buffer : 5% NFDM/TBST

Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-NPR-C antibody [EPR12716] - C-terminal (ab177954) at 1/1000 dilution

All lanes:

Human tissue lysate at 1/10 dilution

Secondary

All lanes:

HRP anti-rabbit, specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 60 kDa

Observed band size: 70 kDa

false

Lab

Western blot - Anti-NPR-C antibody [EPR12716] - C-terminal (AB177954)

Blocking buffer : 5% NFDM/TBST

Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-NPR-C antibody [EPR12716] - C-terminal (ab177954) at 1/7000 dilution

Lane 1:

Mouse kidney tissue lysate at 20 µg

Lane 2:

Rat kidney tissue lysate at 20 µg

Secondary

All lanes:

HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 60 kDa

Observed band size: 70 kDa

false

Lab

Western blot - Anti-NPR-C antibody [EPR12716] - C-terminal (AB177954)

Blocking buffer : 5% NFDM/TBST

Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-NPR-C antibody [EPR12716] - C-terminal (ab177954) at 1/1000 dilution

Lane 1:

Mouse kidney tissue lysate at 20 µg

Lane 2:

Rat kidney tissue lysate at 20 µg

Secondary

All lanes:

HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 60 kDa

Observed band size: 70 kDa

false

不同偶联物与剂型 (5)

665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-NPR-C antibody [EPR12716] - C-terminal
578 PE

PE Anti-NPR-C antibody [EPR12716] - C-terminal
660 APC

APC Anti-NPR-C antibody [EPR12716] - C-terminal
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-NPR-C antibody [EPR12716] - C-terminal
Carrier free

Anti-NPR-C antibody [EPR12716] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR12716

亚型

IgG

不含载体蛋白

反应种属

Mouse, Rat, Human

应用

Flow Cyt (Intra), WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100 - 1/500", "FlowCytIntra-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The NPR-C also known as natriuretic peptide receptor C functions as an important regulator in the homeostasis of cardiovascular and renal functions. It is also referred to as atrial natriuretic peptide receptor C (ANPRC). This protein does not possess guanylyl cyclase activity unlike its counterparts NPR-A and NPR-B but instead acts via clearance binding of natriuretic peptides preventing them from binding to other receptors. The receptor has a molecular mass of approximately 60 kDa and is widely expressed in tissues such as vascular endothelium kidney and adipose tissue.

Biological function summary

NPR-C binds multiple natriuretic peptides including ANP BNP and CNP and modulates their effects by facilitating their clearance from circulation. This receptor does not function as part of a larger protein complex unlike other receptor subtypes. It controls blood volume and pressure by influencing the natriuretic diuretic and vasorelaxant actions of its peptide ligands. NPR-C helps maintain fluid balances and prevents overactivation of hormonal pathways linked to cardiovascular conditions.

Pathways

NPR-C serves an important role in the natriuretic peptide signaling pathway primarily through the regulation of peptide availability. It physically interacts with several circulating natriuretic peptides but not through second messenger cascades. NPR-C is integral to maintaining the balance between the ANP receptor pathway and the renin-angiotensin-aldosterone system therefore indirectly modulating the functions of NPR-A and NPR-B which are responsible for cyclic GMP-dependent signaling.

NPR-C contributes to the pathophysiology of hypertension and heart failure by overseeing the peptide-hormone balance in the cardiovascular system. Anomalies in NPR-C expression or function can lead to improper regulation of blood pressure and fluid retention. Its interaction with proteins such as angiotensin II receptors can exacerbate the effects of hypertension. Understanding the role of NPR-C in these conditions presents potential therapeutic targets for modulating cardiovascular diseases.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Receptor for the natriuretic peptide hormones, binding with similar affinities atrial natriuretic peptide NPPA/ANP, brain natriuretic peptide NPPB/BNP, and C-type natriuretic peptide NPPC/CNP. May function as a clearance receptor for NPPA, NPPB and NPPC, regulating their local concentrations and effects. Acts as a regulator of osteoblast differentiation and bone growth by binding to its ligand osteocrin, thereby preventing binding between NPR3/NPR-C and natriuretic peptides, leading to increase cGMP production (By similarity).

See full target information NPR3

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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

Biomedicines 13: PubMed41007781

2025

Prior Aerobic Exercise Training Fails to Confer Cardioprotection Under Varying Exercise Volumes in Early Post-Infarction Cardiac Remodeling in Female Rats.

Applications

Unspecified application

Species

Unspecified reactive species

André Rodrigues Lourenço Dias,Ednei Luiz Antonio,Helenita Antonia de Oliveira,Ighor Luiz Azevedo Teixeira,Larissa Emília Seibt,Andrey Jorge Serra

Stem cell research & therapy 13:305 PubMed35841070

2022

Metformin combats high glucose-induced damage to the osteogenic differentiation of human periodontal ligament stem cells via inhibition of the NPR3-mediated MAPK pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Lin Zhang,Fen Liu,Zhi-Bang Li,Xiao-Tao He,Xuan Li,Rui-Xin Wu,Hai-Hua Sun,Shao-Hua Ge,Fa-Ming Chen,Ying An

Cellular and molecular life sciences : CMLS 78:2987-3003 PubMed33206203

2020

miR-146a regulates insulin sensitivity via NPR3.

Applications

Unspecified application

Species

Unspecified reactive species

Julian Roos,Meike Dahlhaus,Jan-Bernd Funcke,Monika Kustermann,Gudrun Strauss,Daniel Halbgebauer,Elena Boldrin,Karlheinz Holzmann,Peter Möller,Bernadette M Trojanowski,Bernd Baumann,Klaus-Michael Debatin,Martin Wabitsch,Pamela Fischer-Posovszky

Chinese medical journal 133:1182-1191 PubMed32433050

2020

High salt-induced weakness of anti-oxidative function of natriuretic peptide receptor-C and podocyte damage in the kidneys of Dahl rats.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Long Zhu,Tao Zhang,Zhen-Qiang Xu,Xiao-Chun Ma,Zheng-Jun Wang,Cheng-Wei Zou,Jing-Xin Li,Hai-Yan Jing

Molecular medicine reports 20:5091-5099 PubMed31638216

2019

C‑type natriuretic peptide prevents angiotensin II‑induced atrial connexin 40 and 43 dysregulation by activating AMP‑activated kinase signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Da-Zhi Ding,Ya-Nan Jia,Bo Zhang,Cheng-Ming Guan,Shuai Zhou,Xiang Li,Xun Cui

View all publications

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重组Anti-NPR-C抗体[EPR12716] - C-terminal