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Cardiovascular Blood Other

Anti-NPR-C抗体(ab14355)

  • Datasheet
Reviews (1)Q&A (1)References (6)

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Immunocytochemistry/ Immunofluorescence - Anti-NPR-C antibody (ab14355)

    Key features and details

    • Rabbit polyclonal to NPR-C
    • Suitable for: ICC/IF
    • Reacts with: Human
    • Isotype: IgG

    你可能会感兴趣

    蛋白
    Product image
    Recombinant Human NPR-C protein (ab114355)
    二抗
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    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    查看更多相关产品

    概述

    • 产品名称

      Anti-NPR-C抗体
      参阅全部 NPR-C 一抗
    • 描述

      兔多克隆抗体to NPR-C
    • 宿主

      Rabbit
    • 经测试应用

      适用于: ICC/IFmore details
    • 种属反应性

      与反应: Human
      预测可用于: Mouse, Cow
    • 免疫原

      Synthetic peptide corresponding to Human NPR-C aa 199-212 conjugated to keyhole limpet haemocyanin.
      Sequence:

      QEEGLHSIYSFDET


      (Peptide available as ab49406)
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • 存储溶液

      Constituent: Whole serum
    • Concentration information loading...
    • 纯度

      Whole antiserum
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Cardiovascular
      • Blood
      • Other
      • Signal Transduction
      • Signaling Pathway
      • G Protein Signaling
      • GPCR
      • Signal Transduction
      • Cytoskeleton / ECM
      • Extracellular Matrix
      • Structures
      • Bone
      • Cardiovascular
      • Blood
      • Blood Pressure regulation

    相关产品

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human NPR-C protein (ab114355)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab14355于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    ICC/IF
    1/800.
    说明
    ICC/IF
    1/800.

    靶标

    • 功能

      Receptor for the natriuretic peptide hormones, binding with similar affinities atrial natriuretic peptide NPPA/ANP, brain natriuretic peptide NPPB/BNP, and C-type natriuretic peptide NPPC/CNP. May function as a clearance receptor for NPPA, NPPB and NPPC, regulating their local concentrations and effects. May regulate diuresis, blood pressure and skeletal development. Does not have guanylate cyclase activity.
    • 序列相似性

      Belongs to the ANF receptor family.
    • 细胞定位

      Membrane.
    • Target information above from: UniProt accession P17342 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 281358 Cow
      • Entrez Gene: 4883 Human
      • Entrez Gene: 18162 Mouse
      • Omim: 108962 Human
      • SwissProt: P10730 Cow
      • SwissProt: P17342 Human
      • SwissProt: P70180 Mouse
      • Unigene: 237028 Human
      • Unigene: 619466 Human
      • Unigene: 25259 Mouse
      see all
    • 别名

      • ANP C antibody
      • ANP-C antibody
      • ANPC antibody
      • ANPR-C antibody
      • ANPRC antibody
      • ANPRC_HUMAN antibody
      • Atrial natriuretic peptide C type receptor antibody
      • Atrial natriuretic peptide clearance receptor antibody
      • Atrial natriuretic peptide receptor 3 antibody
      • Atrial natriuretic peptide receptor type C antibody
      • Atrionatriuretic peptide receptor C antibody
      • C5orf23 antibody
      • FLJ14054 antibody
      • GUCY2B antibody
      • MGC22189 antibody
      • Natriuretic peptide receptor C/guanylate cyclase C (atrionatriuretic peptide receptor C) antibody
      • Natriuretic peptide receptor C/guanylate cyclase C antibody
      • NPR 3 antibody
      • NPR C antibody
      • NPR-C antibody
      • Npr3 antibody
      • NPRC antibody
      see all

    图片

    • Immunocytochemistry/ Immunofluorescence - Anti-NPR-C antibody (ab14355)
      Immunocytochemistry/ Immunofluorescence - Anti-NPR-C antibody (ab14355)

      ICC/IF image of ab14355 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14355, purified lot with known concentration equal to 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    实验方案

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet download

      Download

    文献 (6)

    发表研究结果有使用 ab14355?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab14355 被引用在 6 文献中.

    • Mousavi MS  et al. Correlation between adolescent chronic emotional stress and incidence of adult cardiovascular disease in female rats. Iran J Basic Med Sci 22:1179-1185 (2019). PubMed: 31998460
    • Li YX  et al. Role of musclin in the pathogenesis of hypertension in rat. PLoS One 8:e72004 (2013). WB . PubMed: 23940802
    • Abdelalim EM & Tooyama I NPR-C is expressed in the cholinergic and dopaminergic amacrine cells in the rat retina. Peptides 31:180-3 (2010). IHC-FoFr ; Rat . PubMed: 19878700
    • Santos-Araújo C  et al. Local modulation of the natriuretic peptide system in the rat remnant kidney. Nephrol Dial Transplant 24:1774-82 (2009). WB ; Rat . PubMed: 19145001
    • Abdelalim EM  et al. Distribution of natriuretic peptide receptor-C immunoreactivity in the rat brainstem and its relationship to cholinergic and catecholaminergic neurons. Neuroscience 155:192-202 (2008). WB, IHC-FrFl ; Rat . PubMed: 18571869
    • Tian M & Yang XL C-type natriuretic peptide modulates glutamate receptors on cultured rat retinal amacrine cells. Neuroscience 139:1211-20 (2006). WB, ICC/IF ; Rat . PubMed: 16600513

    客户评价及客户问答

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    1-2 of 2 Abreviews or Q&A

    Immunohistochemistry free floating abreview for Anti-NPR-C antibody

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry free floating
    Sample
    Mouse Tissue sections (brain)
    Specification
    brain
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Dec 22 2020

    Question

    Please recommend antibodies that will be specific for extracellular and intracellular epitopes on membrane-bound proteins such as integrins. We are using a mouse cell line, ATDC5.

    Read More

    Abcam community

    Verified customer

    Asked on May 11 2007

    Answer

    Thank you for contacting us. Your question is more difficult than I expected it to be! We have a menu in our Product Browser (located in the upper left-hand corner of our homepage: Tags and Cell Markers/Subcellular Markers/Plasma Membrane) that gives some ideas for antibodies against membrane proteins that may be found in ATDC5 but few that fit your criteria. The pan-cadherin antibodies may be your best choice though they have not been tested for flow cytometry. Antibody ab6529 has been tested in immunocytochemistry, is raised against an intracellular portion of the protein, and reacts with the mouse protein. I expect it can be used for flow cytometry if the cells are fixed and permeabilized, but we cannot guarantee this application. For an antibody that recognizes an extracellular region, the antibodies against the integrins may be useful. Typing "integrin" brings up a list of antibodies that can be filtered by species and application. The ones that have been used for functional studies (FuncS), blocking (BL), or cellular activation will be directed against a portion of the integrin that is extracellular, for instance ab25198 (CD103) or ab36436 (CD61). But these integrins are expressed to different degrees on cell types and have mostly been studied on leukocytes. Various receptors may be useful; a literature search on ATDC5 gave the following link: http://ajplung.physiology.org/cgi/content/full/274/3/L425 The article mentions the natriuretic peptide receptor on ATDC5. We carry several NPR antibodies which can be brought up in a list by searching with our browser for NPR. None have been tested in flow cytometry but ab14355 is raised against an extracellular portion of NPR-C, and ab37620 is raised against a cytoplasmic portion of NPR-B. But this has only been tested for Western blotting, ELISA, and IHC. I hope this helps. Please let me know if you have any questions.

    Read More

    Abcam Scientific Support

    回复于 May 11 2007

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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