重组Anti-NPP-7抗体[EPR12329] (ab174281)
![Western blot - Anti-NPP-7 antibody [EPR12329] (ab174281)](https://www.abcam.cn/ps/products/174/ab174281/Images/ab174281-199272-anti-npp-7-antibody-epr12329-western-blot.jpg "Western blot - Anti-NPP-7 antibody [EPR12329] (ab174281)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12329] to NPP-7
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-NPP-7抗体[EPR12329]
参阅全部 NPP-7 一抗 -
描述
兔单克隆抗体[EPR12329] to NPP-7 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF,IHC-P or IP -
种属反应性
与反应: Human
预测可用于: Mouse
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免疫原
Synthetic peptide within Human NPP-7 aa 350-450 (Cysteine residue). The exact sequence is proprietary.
Database link: Q6UWV6 -
阳性对照
- Caco 2, HT-29 and Human fetal liver lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR12329 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab174281于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/10000 - 1/50000. Predicted molecular weight: 51 kDa.
|
| 说明 |
|---|
|
WB
1/10000 - 1/50000. Predicted molecular weight: 51 kDa. |
应用说明
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
靶标
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功能
Converts sphingomyelin to ceramide. Also has phospholipase C activity toward palmitoyl lyso-phosphocholine. Does not appear to have nucleotide pyrophosphatase activity. -
组织特异性
Detected in the colon (at protein level). Expressed in the duodenum, jejunum and liver and at low levels in the ileum. Expression was very low in the esophagus, stomach and colon. -
序列相似性
Belongs to the nucleotide pyrophosphatase/phosphodiesterase family. -
翻译后修饰
N-glycosylated; required for activity and transport to the plasma membrane. -
细胞定位
Membrane. Localized at the surface of the microvillar membrane in small intestine enterocytes, and in endosome-like structures situated beneath the microvillar membrane, and in Golgi complex. - Information by UniProt
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数据库链接
- Entrez Gene: 339221 Human
- Entrez Gene: 238011 Mouse
- SwissProt: Q6UWV6 Human
- Unigene: 114084 Human
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别名
- ALK SMase antibody
- Alk-SMase antibody
- Alkaline sphingomyelin phosphodiesterase antibody
see all
图片
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Western blot - Anti-NPP-7 antibody [EPR12329] (ab174281)All lanes : Anti-NPP-7 antibody [EPR12329] (ab174281) at 1/10000 dilution
Lane 1 : Caco 2 lysate
Lane 2 : HT-29 lysate
Lane 3 : Human fetal liver lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 51 kDa -
![Anti-NPP-7 antibody [EPR12329] (ab174281)](https://www.abcam.cn/ps/products/174/ab174281/Images/ab174281-2-benefits-of-recombinant-antibodies.png)
Anti-NPP-7 antibody [EPR12329] (ab174281)
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab174281 尚未被引用在任何文献中。