AB174281

重组Anti-NPP-7抗体[EPR12329]

Anti-NPP-7 antibody [EPR12329]

RabMAb
Recombinant
了解详情

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(1 Publication)

Rabbit Recombinant Monoclonal NPP-7 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

查看别名

UNQ3077/PRO9912, ENPP7, Ectonucleotide pyrophosphatase/phosphodiesterase family member 7, E-NPP 7, NPP-7, Alkaline sphingomyelin phosphodiesterase, Intestinal alkaline sphingomyelinase, Alk-SMase

1 Images

Supplier Data

Western blot - Anti-NPP-7 antibody [EPR12329] (AB174281)

All lanes:

Western blot - Anti-NPP-7 antibody [EPR12329] (ab174281) at 1/10000 dilution

Lane 1:

Caco 2 lysate at 10 µg

Lane 2:

HT-29 lysate at 10 µg

Lane 3:

Human fetal liver lysate at 10 µg

Predicted band size: 51 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-NPP-7 antibody [EPR12329] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR12329

亚型

IgG

不含载体蛋白

反应种属

Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

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性能和储存信息

形式

Liquid

纯度

Tissue culture supernatant

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

NPP-7 also known as nucleotide pyrophosphatase/phosphodiesterase 7 is an enzyme involved in the hydrolysis of pyrophosphate-containing substrates. It has a molecular mass of approximately 125 kDa. This enzyme exhibits expression in several human tissues prominently in the intestinal tract and kidney. The enzyme's activity facilitates the breakdown of molecules serving roles in both digestion and cellular signaling processes.

Biological function summary

NPP-7 participates in lipid metabolism and contributes to maintaining homeostasis by degrading extracellular nucleotides. It forms part of larger protein complexes that regulate various cellular activities. Its enzymatic function is important for controlling the concentration of lipid-derived signaling molecules thereby impacting cellular communication and response mechanisms.

Pathways

NPP-7 integrates into the pathway regulating phospholipid metabolism and nucleotide salvage pathways. It interacts with proteins such as NPP-2 which are involved in similar metabolic processes ensuring balance in lipid signaling and energy storage within cells. Its function in these pathways highlights its role in modulating phospholipid levels and influencing liver function and cellular energy dynamics.

NPP-7 relates to conditions such as metabolic syndrome and chronic kidney disease. Dysregulation or altered expression of NPP-7 can affect lipid metabolism and kidney function contributing to these diseases. This protein interacts with other proteins including ENPP1 which ties into its involvement in disrupting normal cellular metabolism and exacerbating pathological states in these conditions.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Choline-specific phosphodiesterase that hydrolyzes sphingomyelin releasing the ceramide and phosphocholine and therefore is involved in sphingomyelin digestion, ceramide formation, and fatty acid (FA) absorption in the gastrointestinal tract (PubMed : 12671034, PubMed : 12885774, PubMed : 15205117, PubMed : 16255717, PubMed : 28292932). Also has phospholipase C activity and can also cleave phosphocholine from palmitoyl lyso-phosphatidylcholine and platelet-activating factor (PAF) leading to its inactivation (PubMed : 12885774, PubMed : 16255717). Does not have nucleotide pyrophosphatase activity (PubMed : 12885774). May promote cholesterol absorption by affecting the levels of sphingomyelin derived from either diet or endogenous sources, in the intestinal lumen (By similarity).

See full target information ENPP7

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

PLoS pathogens 21:e1012772 PubMed40522989

2025

Env from EIAV vaccine delicately regulates NLRP3 activation via attenuating NLRP3-NEK7 interaction.

Applications

Unspecified application

Species

Unspecified reactive species

Xing Guo,Cong Liu,Yuhong Wang,Hongxin Li,Saiwen Ma,Lei Na,Huiling Ren,Yuezhi Lin,Xiaojun Wang

View all publications

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重组Anti-NPP-7抗体[EPR12329]