Anti-NPL4抗体(ab224435)
Key features and details
- Rabbit polyclonal to NPL4
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-NPL4抗体
参阅全部 NPL4 一抗 -
描述
兔多克隆抗体to NPL4 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, ICC/IF, WBmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment corresponding to Human NPL4 aa 350-550.
Database link: Q8TAT6 -
阳性对照
- WB: A549, NIH/3T3 and NBT-II cell lysates. IHC-P: Human skeletal muscle and testis tissue. ICC/IF: A431 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab224435于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
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| WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 68 kDa.
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| 说明 |
|---|
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IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 68 kDa. |
靶标
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功能
The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. -
组织特异性
Expressed at highest levels in brain, heart, skeletal muscle, kidney and fetal liver. -
通路
Protein degradation; proteasomal ubiquitin-dependent pathway. -
序列相似性
Belongs to the NPL4 family.
Contains 1 RanBP2-type zinc finger. -
结构域
Binds ubiquitinated proteins via its RanBP2-type zinc finger. -
细胞定位
Cytoplasm > cytosol. Endoplasmic reticulum. Nucleus. Associated with the endoplasmic reticulum and nuclear. - Information by UniProt
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数据库链接
- Entrez Gene: 55666 Human
- Entrez Gene: 217365 Mouse
- Entrez Gene: 140639 Rat
- Omim: 606590 Human
- SwissProt: Q8TAT6 Human
- SwissProt: P60670 Mouse
- SwissProt: Q9ES54 Rat
- Unigene: 464333 Human
see all -
别名
- KIAA1499 antibody
- NPL4 antibody
- NPL4, S. cerevisiae, homolog of antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPL4 antibody (ab224435)
Paraffin-embedded human skeletal muscle tissue stained for NPL4 using ab224435 at 1/20 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPL4 antibody (ab224435)Paraffin-embedded human testis tissue stained for NPL4 using ab224435 at 1/20 dilution in immunohistochemical analysis.
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Western blot - Anti-NPL4 antibody (ab224435)Anti-NPL4 antibody (ab224435) at 1/100 dilution + A549 (human lung carcinoma cell line) cell lysate
Developed using the ECL technique.
Predicted band size: 68 kDa -
Western blot - Anti-NPL4 antibody (ab224435)All lanes : Anti-NPL4 antibody (ab224435) at 1/100 dilution
Lane 1 : NIH/3T3 (mouse embryonic fibroblast cell line) cell lysate
Lane 2 : NBT-II cell lysate
Developed using the ECL technique.
Predicted band size: 68 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-NPL4 antibody (ab224435)PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for NPL4 (green) using ab224435 at 4 µg/ml in ICC/IF.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab224435 被引用在 2 文献中.
- Wang N et al. NPLOC4 is a potential target and a poor prognostic signature in lung squamous cell carcinoma. Sci Rep 13:20430 (2023). PubMed: 37993584
- Liu Y et al. Disulfiram/Copper induces antitumor activity against gastric cancer via the ROS/MAPK and NPL4 pathways. Bioengineered 13:6579-6589 (2022). PubMed: 35290151