Anti-NPHS2抗体(ab177242)
Key features and details
- Goat polyclonal to NPHS2
- Suitable for: WB
- Reacts with: Mouse, Human, Pig
- Isotype: IgG
概述
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产品名称
Anti-NPHS2抗体
参阅全部 NPHS2 一抗 -
描述
山羊多克隆抗体to NPHS2 -
宿主
Goat -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Human, Pig
预测可用于: Cow
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免疫原
Synthetic peptide corresponding to Human NPHS2 aa 365-377 (C terminal) (Cysteine residue).
Sequence:SPSKPVEPLNPKK
Database link: NP_055440.1 -
阳性对照
- WB: Mouse, human and pig kidney lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab177242 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab177242于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 42 kDa).
1 hour primary incubation is recommended for this product. |
| 说明 |
|---|
|
WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 42 kDa). 1 hour primary incubation is recommended for this product. |
靶标
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功能
Plays a role in the regulation of glomerular permeability, acting probably as a linker between the plasma membrane and the cytoskeleton. -
组织特异性
Almost exclusively expressed in the podocytes of fetal and mature kidney glomeruli. -
疾病相关
Defects in NPHS2 are the cause of nephrotic syndrome type 2 (NPHS2) [MIM:600995]. It is a renal disorder characterized clinically by childhood onset of proteinuria, hypoalbuminemia, hyperlipidemia, and edema. Kidney biopsies show non-specific histologic changes such as focal segmental glomerulosclerosis and diffuse mesangial proliferation. The disorder is resistant to steroid treatment and progresses to end-stage renal failure in the first or second decades. Some patients show later onset of the disorder. -
序列相似性
Belongs to the band 7/mec-2 family. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 537633 Cow
- Entrez Gene: 7827 Human
- Entrez Gene: 170484 Mouse
- Omim: 604766 Human
- SwissProt: Q9NP85 Human
- SwissProt: Q91X05 Mouse
- Unigene: 412710 Human
- Unigene: 289099 Mouse
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别名
- nephrosis 2 antibody
- Nephrosis 2, idiopathic, steroid resistant (podocin) antibody
- nephrosis 2, idiopathic, steroid resistant antibody
see all
图片
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Western blot - Anti-NPHS2 antibody (ab177242)All lanes : Anti-NPHS2 antibody (ab177242) at 0.3 µg/ml
Lane 1 : Human kidney lysate (in RIPA buffer)
Lane 2 : Mouse kidney lysate (in RIPA buffer)
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 42 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour.
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Western blot - Anti-NPHS2 antibody (ab177242)Anti-NPHS2 antibody (ab177242) at 1 µg/ml + Pig kidney lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 42 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab177242 尚未被引用在任何文献中。