AB307837

重组Anti-NPAT抗体[27/NPAT]

Anti-NPAT antibody [27/NPAT]

Recombinant
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Mouse Recombinant Monoclonal NPAT antibody. Suitable for ICC/IF and reacts with Human samples.

查看别名

CAND3, E14, NPAT, Protein NPAT, Nuclear protein of the ataxia telangiectasia mutated locus, p220, Nuclear protein of the ATM locus

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-NPAT antibody [27/NPAT] (AB307837)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NPAT antibody [27/NPAT] (AB307837)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1%Triton X-100 permeabilized Saos-2 (human osteosarcoma epithelial cell) cells labelling NPAT with ab307837 at 1/250 dilution (3.99 ug/ml), followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 ug/ml) (Green). Confocal image showing nuclear foci staining in Saos-2 cell line. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab206369 Anti-beta Tubulin rabbit monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 dilution (5 ug/ml) (Red). The nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/ml).

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NPAT antibody [27/NPAT] (AB307837)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1%Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell) cells labelling NPAT with ab307837 at 1/250 dilution (3.99 ug/ml), followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2ug/ml) (Green). Confocal image showing nuclear foci staining in U-2 OS cell line. The number of positive-staining cells decreased after treatment with 20 uM roscovitine for 48 h (PMID : 14976556). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab206369 Anti-beta Tubulin rabbit monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 dilution (5ug/ml) (Red). The nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2ug/ml).

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NPAT antibody [27/NPAT] (AB307837)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1%Triton X-100 permeabilized A431 (human epidermoid carcinoma epithelial cell) cells labelling NPAT with ab307837 at 1/250 dilution (3.99 ug/ml), followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2ug/ml) (Green). Confocal image showing nuclear foci staining in A431 cell line. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab206369 Anti-beta Tubulin rabbit monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (10ug/ml) (Red). The nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2ug/ml).

不同偶联物与剂型 (1)

Carrier free

Anti-NPAT antibody [27/NPAT] - BSA and Azide free

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

27/NPAT

亚型

IgG2b

不含载体蛋白

反应种属

Human

应用

ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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产品详情

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

NPAT also known as nuclear protein ataxia-telangiectasia locus is a protein that weighs approximately 220 kDa. It is widely present in various tissues with a high expression in proliferating cells. NPAT participates in cell cycle regulation and links directly to the control of histone gene expression during the G1/S phase transition. Its presence in the nuclear matrix further highlights its integrative role in gene regulation.

Biological function summary

NPAT plays an important role in the cell cycle's advancement by associating with the MMB (Myb-MuvB) complex. This interaction signifies its importance in DNA replication and cell cycle progression. NPAT activates transcription upon phosphorylation by CDK2/cyclin E influencing S-phase entry. It acts as a transcriptional regulator for histone gene expression necessary for chromatin structure and genome stability.

Pathways

NPAT integrates into the cyclin/CDK regulatory pathway governing cell cycle progression and is related to histone gene clusters regulation. The interaction with CDK2 illustrates its part in the regulation of cell proliferation. NPAT also associates with the E2F cell cycle pathway further influencing gene transcription important for DNA replication.

NPAT relates to cancer and ataxia-telangiectasia. These connections highlight its importance in cell cycle control disruption which can lead to oncogenesis. NPAT's relationship with the ATM protein emerges in the context of ataxia-telangiectasia suggesting a link between DNA damage response defects and neurological disorders. These associations reinforce NPAT’s involvement in maintaining cellular health and genomic integrity.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Transcription regulator required for progression through the G1 and S phases of the cell cycle and for S phase entry (PubMed : 12665581, PubMed : 15555599, PubMed : 9472014). Acts as a key transcription regulator of histones (PubMed : 10995386, PubMed : 10995387, PubMed : 12724424, PubMed : 14585971, PubMed : 14612403, PubMed : 15988025, PubMed : 16131487, PubMed : 17163457, PubMed : 25339177, PubMed : 40516528, PubMed : 40516529). Activates transcription of the histone H2A, histone H2B, histone H3 and histone H4 genes in conjunction with GON4L and MIZF (PubMed : 10995386, PubMed : 10995387, PubMed : 12724424, PubMed : 14585971, PubMed : 14612403, PubMed : 15988025, PubMed : 16131487, PubMed : 17163457, PubMed : 25339177). Together with CRAMP1, binds to the promoters of H1 genes (H1-2, H1-3, H1-4, H1-5 and H1-10/H1x), driving their transcription (PubMed : 40516528, PubMed : 40516529). Also positively regulates the ATM, MIZF and PRKDC promoters (PubMed : 17826007, PubMed : 17974976, PubMed : 8743993, PubMed : 8923007). Transcriptional activation may be accomplished at least in part by the recruitment of the NuA4 histone acetyltransferase (HAT) complex to target gene promoters (PubMed : 17967892).

See full target information NPAT

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重组Anti-NPAT抗体[27/NPAT]