AB109984

Anti-Npas4抗体

Name: Npas4抗体 (ab109984)| Abcam中文官网
Brand: Abcam Limited
SKU: AB109984
Price: 4829 CNY
Availability: InStock

Anti-Npas4 antibody

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(4 Publications)

Goat Polyclonal NPAS4 antibody. Suitable for WB and reacts with Mouse, Human, Rat samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human NPAS4 aa 100-200.

查看别名

BHLHE79, NXF, PASD10, NPAS4, Neuronal PAS domain-containing protein 4, Neuronal PAS4, Class E basic helix-loop-helix protein 79, HLH-PAS transcription factor NXF, PAS domain-containing protein 10, bHLHe79

3 Images

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Western blot - Anti-Npas4 antibody (AB109984)

All lanes:

Western blot - Anti-Npas4 antibody (ab109984) at 1 µg/mL

All lanes:

Human frontal cortex lysate (in RIPA buffer) at 35 µg

Predicted band size: 87 kDa

Observed band size: 90 kDa

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Supplier Data

Western blot - Anti-Npas4 antibody (AB109984)

All lanes:

Western blot - Anti-Npas4 antibody (ab109984) at 1 µg/mL

Lane 1:

Mouse brain lysate at 35 µg

Lane 2:

Rat brain lysate at 35 µg

Predicted band size: 87 kDa

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CiteAb

Western blot - Anti-Npas4 antibody (AB109984)

Npas4 western blot using anti-Npas4 antibody ab109984. Publication image and figure legend from Zhang, Z., Wang, M., et al., 2018, Cell Res, PubMed 30297870.

ab109984 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab109984 please see the product overview.

m6A promotes the translation of immediate-early genes upon activity induction. a Immediate-early genes (IEGs) are comparably induced by fear conditioning training in the hippocampus of both the CTRL (blue) and cKO (red) mice. b Increment of m6A modification abundance on IEG mRNAs after fear conditioning training in hippocampus samples of the CTRL mice but not the Mettl3 cKO mice. IP, immunoprecipitated fraction. c Translation of IEGs is impaired in the cKO mice after training. d Immunofluorescent image of EGR1 in CA1 region before and after fear conditioning training (1 h and 4 h). e Translation of IEGs is impaired in Mettl3-KO primary cortical neurons, which can be rescued by re-expression of wildtype Mettl3 but not mutated Mettl3. Student's t-test, *p < 0.05, **p < 0.01, ***p < 0.001, N.S., not significant, (a) n = 2 replicates, (b), (c) and (e) n = 3 replicates

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关键信息

宿主种属

Goat

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Mouse, Rat, Human

应用

applications

免疫原

Synthetic Peptide within Human NPAS4 aa 100-200. The exact immunogen used to generate this antibody is proprietary information.

Q8IUM7

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.3-1 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.3-1 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product." }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.3-1 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product." }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

纯化说明

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

存储溶液

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Npas4 also known as Neuronal PAS domain protein 4 functions mechanically as a transcription factor. It weighs approximately 88 kDa. Scientists find Npas4 expression primarily in the brain particularly in regions involved with learning and memory processes. This protein plays an important role in regulating the expression of other genes especially in neurons.

Biological function summary

Npas4 modulates the response of neurons to activity. It is not part of a larger complex but interacts with various partners to achieve its function. Npas4 activates specific gene programs that control synaptic plasticity supporting the strengthening or weakening of synapses. This helps maintain excitatory-inhibitory balance in neural circuits a critical process for proper brain function.

Pathways

Researchers find Npas4's role integral in the signaling cascades involved in synaptic plasticity and experience-dependent neuronal development. It works closely with pathways like calcium signaling where Npas4 acts in response to changes in calcium levels to alter gene expression. The BDNF protein known for its role in neuronal growth is significantly influenced by Npas4 activity in these pathways highlighting its importance in neural adaptation.

Npas4 shows a connection to mental health conditions. Impaired Npas4 function relates to anxiety disorders where altered synaptic plasticity plays an important role. Additionally its dysregulation has links to neurological disorders like epilepsy due to its part in maintaining synaptic balance. Aberrant Npas4 expression influences these disorders and often involves interactions with GABAergic signaling pathway proteins affecting the overall excitatory and inhibitory dynamics in neuronal networks.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Transcription factor expressed in neurons of the brain that regulates the excitatory-inhibitory balance within neural circuits and is required for contextual memory in the hippocampus (By similarity). Plays a key role in the structural and functional plasticity of neurons (By similarity). Acts as an early-response transcription factor in both excitatory and inhibitory neurons, where it induces distinct but overlapping sets of late-response genes in these two types of neurons, allowing the synapses that form on inhibitory and excitatory neurons to be modified by neuronal activity in a manner specific to their function within a circuit, thereby facilitating appropriate circuit responses to sensory experience (By similarity). In excitatory neurons, activates transcription of BDNF, which in turn controls the number of GABA-releasing synapses that form on excitatory neurons, thereby promoting an increased number of inhibitory synapses on excitatory neurons (By similarity). In inhibitory neurons, regulates a distinct set of target genes that serve to increase excitatory input onto somatostatin neurons, probably resulting in enhanced feedback inhibition within cortical circuits (By similarity). The excitatory and inhibitory balance in neurons affects a number of processes, such as short-term and long-term memory, acquisition of experience, fear memory, response to stress and social behavior (By similarity). Acts as a regulator of dendritic spine development in olfactory bulb granule cells in a sensory-experience-dependent manner by regulating expression of MDM2 (By similarity). Efficient DNA binding requires dimerization with another bHLH protein, such as ARNT, ARNT2 or BMAL1 (PubMed : 14701734). Can activate the CME (CNS midline enhancer) element (PubMed : 14701734).

See full target information NPAS4

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Brain and behavior 15:e70377 PubMed40320842

2025

Analysis and Identification of Therapeutic Targets for Neuronal Regeneration After Ischemic Stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Li Min,Li Guo,Zhenyu Wang,Lei Zhao,Chenglong Shi,Xiaoyong Liu,Zhen Wang,Fei-Fei Shang,Jiaping Wang

Scientific reports 10:17935 PubMed33087769

2020

Repeated stress exposure in mid-adolescence attenuates behavioral, noradrenergic, and epigenetic effects of trauma-like stress in early adult male rats.

Applications

Unspecified application

Species

Unspecified reactive species

Lauren E Chaby,Nareen Sadik,Nicole A Burson,Scott Lloyd,Kelly O'Donnel,Jesse Winters,Alana C Conti,Israel Liberzon,Shane A Perrine

Cell research 28:1050-1061 PubMed30297870

2018

METTL3-mediated N-methyladenosine mRNA modification enhances long-term memory consolidation.

Applications

Species

Mouse

Zeyu Zhang,Meng Wang,Dongfang Xie,Zenghui Huang,Lisha Zhang,Ying Yang,Dongxue Ma,Wenguang Li,Qi Zhou,Yun-Gui Yang,Xiu-Jie Wang

Frontiers in systems neuroscience 12:35 PubMed30087600

2018

Hippocampal Mechanisms Underlying Impairment in Spatial Learning Long After Establishment of Noise-Induced Hearing Loss in CBA Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Lijie Liu,Chuanying Xuan,Pei Shen,Tingting He,Ying Chang,Lijuan Shi,Shan Tao,Zhiping Yu,Richard E Brown,Jian Wang

View all publications

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Anti-Npas4抗体

Anti-Npas4 antibody

Western blot - Anti-Npas4 antibody (AB109984)

Western blot - Anti-Npas4 antibody (AB109984)

Western blot - Anti-Npas4 antibody (AB109984)