重组Anti-NOXA2/p67phox抗体[EPR5065] (ab109523)
![Western blot - Anti-NOXA2/p67phox antibody [EPR5065] (ab109523)](https://www.abcam.cn/ps/products/109/ab109523/Images/ab109523-122449-anti-noxa2-p67phox-antibody-epr5065-western-blot.jpg "Western blot - Anti-NOXA2/p67phox antibody [EPR5065] (ab109523)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5065] to NOXA2/p67phox
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-NOXA2/p67phox抗体[EPR5065]
参阅全部 NOXA2/p67phox 一抗 -
描述
兔单克隆抗体[EPR5065] to NOXA2/p67phox -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, IHC-Pmore details
不适用于: IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- THP-1 or HL-60 treated with Dexomethasone and TPA cell lysate Human colon tissue
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5065 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109523于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/10000 - 1/50000. Detects a band of approximately 67 kDa (predicted molecular weight: 60 kDa).
|
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| IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/10000 - 1/50000. Detects a band of approximately 67 kDa (predicted molecular weight: 60 kDa). |
|
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production). -
疾病相关
Defects in NCF2 are a cause of chronic granulomatous disease autosomal recessive cytochrome-b-positive type 2 (CGD2) [MIM:233710]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections. -
序列相似性
Belongs to the NCF2/NOXA1 family.
Contains 1 OPR domain.
Contains 2 SH3 domains.
Contains 3 TPR repeats. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 4688 Human
- Omim: 608515 Human
- SwissProt: P19878 Human
- Unigene: 587558 Human
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别名
- 67 kDa neutrophil oxidase factor antibody
- Chronic granulomatous disease autosomal 2 antibody
- FLJ93058 antibody
see all
图片
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Western blot - Anti-NOXA2/p67phox antibody [EPR5065] (ab109523)All lanes : Anti-NOXA2/p67phox antibody [EPR5065] (ab109523) at 1/10000 dilution
Lane 1 : THP-1 cell lysate
Lane 2 : HL-60 treated with Dexomethasone and TPA cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 60 kDa
Observed band size: 67 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOXA2/p67phox antibody [EPR5065] (ab109523)](https://www.abcam.cn/ps/products/109/ab109523/Images/ab109523-122448-anti-noxa2-p67phox-antibody-epr5065-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOXA2/p67phox antibody [EPR5065] (ab109523)ab109523, at a 1/500 dilution, staining Human NOXA2/p67phox in colon, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Flow Cytometry (Intracellular) - Anti-NOXA2/p67phox antibody [EPR5065] (ab109523)](https://www.abcam.cn/ps/products/109/ab109523/Images/ab109523-122447-anti-noxa2-p67phox-antibody-epr5065-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-NOXA2/p67phox antibody [EPR5065] (ab109523)Intracellular Flow Cytometry analysis of permeabilized THP-1 cells using ab109523 (red) and a rabbit IgG (negative contol) (green). The dilution is 1/150. The secondary antibody is a goat anti-rabbit IgG FITC.
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![Anti-NOXA2/p67phox antibody [EPR5065] (ab109523)](https://www.abcam.cn/ps/products/109/ab109523/Images/ab109523-4-benefits-of-recombinant-antibodies.png)
Anti-NOXA2/p67phox antibody [EPR5065] (ab109523)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab109523 被引用在 3 文献中.
- Bakri FG et al. Second Report of Chronic Granulomatous Disease in Jordan: Clinical and Genetic Description of 31 Patients From 21 Different Families, Including Families From Lybia and Iraq. Front Immunol 12:639226 (2021). PubMed: 33746979
- Brault J et al. Ex Vivo Models of Chronic Granulomatous Disease. Methods Mol Biol 1982:587-622 (2019). PubMed: 31172497
- Hayashi G & Cortopassi G Lymphoblast Oxidative Stress Genes as Potential Biomarkers of Disease Severity and Drug Effect in Friedreich's Ataxia. PLoS One 11:e0153574 (2016). WB . PubMed: 27078885