重组Anti-NOXA2/p67phox抗体[EPR5064] (ab109366)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5064] to NOXA2/p67phox
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-NOXA2/p67phox抗体[EPR5064]
参阅全部 NOXA2/p67phox 一抗 -
描述
兔单克隆抗体[EPR5064] to NOXA2/p67phox -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Human spleen tissue. THP-1 cell lysate. HL-60 cells treated with dexomethasome, dimethyl sulfoxide, and TPA
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5064 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109366于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (3) |
1/1000 - 1/10000. Predicted molecular weight: 60 kDa.
|
IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 60 kDa. |
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production). -
疾病相关
Defects in NCF2 are a cause of chronic granulomatous disease autosomal recessive cytochrome-b-positive type 2 (CGD2) [MIM:233710]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections. -
序列相似性
Belongs to the NCF2/NOXA1 family.
Contains 1 OPR domain.
Contains 2 SH3 domains.
Contains 3 TPR repeats. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 4688 Human
- Entrez Gene: 17970 Mouse
- Entrez Gene: 364018 Rat
- Omim: 608515 Human
- SwissProt: P19878 Human
- SwissProt: O70145 Mouse
- SwissProt: Q3U5S4 Mouse
- SwissProt: A7E3N2 Rat
see all -
别名
- 67 kDa neutrophil oxidase factor antibody
- Chronic granulomatous disease autosomal 2 antibody
- FLJ93058 antibody
see all
图片
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All lanes : Anti-NOXA2/p67phox antibody [EPR5064] (ab109366) at 1/1000 dilution
Lane 1 : HL-60 cells treated with dexomethasome, dimethyl sulfoxide, and TPA
Lane 2 : THP-1 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti-Rabbit at 1/2000 dilution
Predicted band size: 60 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOXA2/p67phox antibody [EPR5064] (ab109366)
Immunohistochemical analysis of NOXA2/p67phox in paraffin-embedded Human spleen tissue using ab109366 at 1/500 dilution
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (16)
ab109366 被引用在 16 文献中.
- Huang N et al. Role of NCF2 as a potential prognostic factor and immune infiltration indicator in hepatocellular carcinoma. Cancer Med 12:8991-9004 (2023). PubMed: 36680322
- Ma LL et al. Bioinformatics analysis of potential key ferroptosis-related genes involved in tubulointerstitial injury in patients with diabetic nephropathy. Ren Fail 45:2199095 (2023). PubMed: 37038746
- Luo LP et al. Shenkang Injection and Its Three Anthraquinones Ameliorates Renal Fibrosis by Simultaneous Targeting IƙB/NF-ƙB and Keap1/Nrf2 Signaling Pathways. Front Pharmacol 12:800522 (2021). PubMed: 35002735
- Yang X et al. Enhanced cardiomyocyte reactive oxygen species signaling promotes ibrutinib-induced atrial fibrillation. Redox Biol 30:101432 (2020). PubMed: 31986467
- Duan M et al. Indigo Fruits Ingredient, Aucubin, Protects against LPS-Induced Cardiac Dysfunction in Mice. J Pharmacol Exp Ther 371:348-359 (2019). PubMed: 31467086