Anti-NOXA1抗体(ab68523)
Key features and details
- Mouse polyclonal to NOXA1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-NOXA1抗体
参阅全部 NOXA1 一抗 -
描述
小鼠多克隆抗体to NOXA1 -
宿主
Mouse -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein within Human NOXA1. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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阳性对照
- NOXA1 transfected 293T lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Constituent: 100% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab68523于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 51 kDa).
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| 说明 |
|---|
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WB
1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 51 kDa). |
靶标
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功能
Functions as an activator of NOX1, a superoxide-producing NADPH oxidase. Functions in the production of reactive oxygen species (ROS) which participate in a variety of biological processes including host defense, hormone biosynthesis, oxygen sensing and signal transduction. May also activate CYBB/gp91phox and NOX3. -
组织特异性
Widely expressed. Detected in pancreas, liver, kidney, spleen, prostate, small intestine and colon. -
序列相似性
Belongs to the NCF2/NOXA1 family.
Contains 1 OPR domain.
Contains 1 SH3 domain.
Contains 4 TPR repeats. -
发展阶段
Expressed in fetal kidney. -
结构域
The SH3 domain mediates interaction with NOXO1 and NCF1 and has autoregulatory function.
The TPR repeats mediate interaction with RAC1. -
翻译后修饰
Interaction with YWHAZ depends on phosphorylation by PKA. -
细胞定位
Cytoplasm. Cell membrane. Translocation to membranes depends on NOXO1 or NCF1 and maybe RAC1. - Information by UniProt
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数据库链接
- Entrez Gene: 10811 Human
- Omim: 611255 Human
- SwissProt: Q86UR1 Human
- Unigene: 495554 Human
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别名
- Antigen NY CO 31 antibody
- Antigen NY-CO-31 antibody
- FLJ25475 antibody
see all
图片
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Western blot - Anti-NOXA1 antibody (ab68523)All lanes : Anti-NOXA1 antibody (ab68523) at 1/500 dilution
Lane 1 : NOXA1 transfected 293T lysate
Lane 2 : Non-transfected 293T lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size: 51 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
数据表及文件
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Datasheet download
文献 (5)
ab68523 被引用在 5 文献中.
- De Bessa TC et al. Subverted regulation of Nox1 NADPH oxidase-dependent oxidant generation by protein disulfide isomerase A1 in colon carcinoma cells with overactivated KRas. Cell Death Dis 10:143 (2019). PubMed: 30760703
- Chocry M et al. Reversion of resistance to oxaliplatin by inhibition of p38 MAPK in colorectal cancer cell lines: involvement of the calpain / Nox1 pathway. Oncotarget 8:103710-103730 (2017). PubMed: 29262595
- Streeter J et al. Phosphorylation of Nox1 regulates association with NoxA1 activation domain. Circ Res 115:911-8 (2014). PubMed: 25228390
- Csányi G et al. Nox2 B-loop peptide, Nox2ds, specifically inhibits the NADPH oxidase Nox2. Free Radic Biol Med 51:1116-25 (2011). WB ; Human . PubMed: 21586323
- Biasi F et al. Pro-oxidant and proapoptotic effects of cholesterol oxidation products on human colonic epithelial cells: a potential mechanism of inflammatory bowel disease progression. Free Radic Biol Med 47:1731-41 (2009). IP ; Human . PubMed: 19778604
