Anti-NOXA1抗体(ab222852)
Key features and details
- Rabbit polyclonal to NOXA1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-NOXA1抗体
参阅全部 NOXA1 一抗 -
描述
兔多克隆抗体to NOXA1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant fragment corresponding to Human NOXA1 aa 150-313.
Database link: Q86UR1 -
阳性对照
- WB: Mouse brain lysate. IHC: Human lung cancer and kidney tissues. ICC/IF: HepG2 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Protein G purified -
纯化说明
Purity >95%. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab222852于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/5000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
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| IHC-P |
1/20 - 1/200.
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| ICC/IF |
1/50 - 1/200.
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| 说明 |
|---|
|
WB
1/1000 - 1/5000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa). |
|
IHC-P
1/20 - 1/200. |
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ICC/IF
1/50 - 1/200. |
靶标
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功能
Functions as an activator of NOX1, a superoxide-producing NADPH oxidase. Functions in the production of reactive oxygen species (ROS) which participate in a variety of biological processes including host defense, hormone biosynthesis, oxygen sensing and signal transduction. May also activate CYBB/gp91phox and NOX3. -
组织特异性
Widely expressed. Detected in pancreas, liver, kidney, spleen, prostate, small intestine and colon. -
序列相似性
Belongs to the NCF2/NOXA1 family.
Contains 1 OPR domain.
Contains 1 SH3 domain.
Contains 4 TPR repeats. -
发展阶段
Expressed in fetal kidney. -
结构域
The SH3 domain mediates interaction with NOXO1 and NCF1 and has autoregulatory function.
The TPR repeats mediate interaction with RAC1. -
翻译后修饰
Interaction with YWHAZ depends on phosphorylation by PKA. -
细胞定位
Cytoplasm. Cell membrane. Translocation to membranes depends on NOXO1 or NCF1 and maybe RAC1. - Information by UniProt
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数据库链接
- Entrez Gene: 10811 Human
- Entrez Gene: 241275 Mouse
- Omim: 611255 Human
- SwissProt: Q86UR1 Human
- SwissProt: Q8CJ00 Mouse
- Unigene: 495554 Human
- Unigene: 218774 Mouse
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别名
- Antigen NY CO 31 antibody
- Antigen NY-CO-31 antibody
- FLJ25475 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOXA1 antibody (ab222852)
Paraffin-embedded human lung cancer tissue stained for NOXA1 using ab222852 at 1/100 dilution in immunohistochemical analysis.
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Western blot - Anti-NOXA1 antibody (ab222852)Anti-NOXA1 antibody (ab222852) at 1/1000 dilution + Mouse brain lysate
Secondary
Goat polyclonal to rabbit at 1/10000 dilution
Predicted band size: 51 kDa
Observed band size: 51 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOXA1 antibody (ab222852)Paraffin-embedded human kidney tissue stained for NOXA1 using ab222852 at 1/100 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-NOXA1 antibody (ab222852)HepG2 (human liver hepatocellular carcinoma cell line) cells stained for NOXA1 (green) using ab222852 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® congugated Goat Anti-Rabbit IgG (H+L).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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