Anti-NOX2/gp91phox抗体[54.1] (ab80897)
![Western blot - Anti-NOX2/gp91phox antibody [54.1] (ab80897)](https://www.abcam.cn/ps/products/80/ab80897/Images/ab80897-96144-anti-nox2-gp91phox-antibody-541-western-blot.jpg "Western blot - Anti-NOX2/gp91phox antibody [54.1] (ab80897)")
Key features and details
- Mouse monoclonal [54.1] to NOX2/gp91phox
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG1
概述
-
产品名称
Anti-NOX2/gp91phox抗体[54.1]
参阅全部 NOX2/gp91phox 一抗 -
描述
小鼠单克隆抗体[54.1] to NOX2/gp91phox -
宿主
Mouse -
特异性
This antibody is specific for NOX2/gp91phox 382-PKIAVDGP-389. -
经测试应用
适用于: WB, ICC/IF, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Full length protein. This information is proprietary to Abcam and/or its suppliers.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Protein G purified -
克隆
单克隆 -
克隆编号
54.1 -
骨髓瘤
Sp2/0 -
同种型
IgG1 -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab80897于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).
|
|
| ICC/IF |
Use a concentration of 1 µg/ml.
|
|
| IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
WB
Use at an assay dependent concentration. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa). |
|
ICC/IF
Use a concentration of 1 µg/ml. |
|
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
-
功能
Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc. -
疾病相关
Defects in CYBB are a cause of chronic granulomatous disease X-linked (XCGD) [MIM:306400]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections. -
序列相似性
Contains 1 FAD-binding FR-type domain.
Contains 1 ferric oxidoreductase domain. -
翻译后修饰
Glycosylated. -
细胞定位
Membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 1536 Human
- Omim: 300481 Human
- SwissProt: P04839 Human
- Unigene: 292356 Human
-
别名
- AMCBX2 antibody
- CGD antibody
- CGD91-phox antibody
see all
图片
-
Western blot - Anti-NOX2/gp91phox antibody [54.1] (ab80897)All lanes : Anti-NOX2/gp91phox antibody [54.1] (ab80897) at 1 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : Human lymph node tissue lysate - total protein (ab29871)
Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 65 kDa
Observed band size: 65 kDa
Additional bands at: 29 kDa, 35 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes -
![Immunocytochemistry/ Immunofluorescence - Anti-NOX2/gp91phox antibody [54.1] (ab80897)](https://www.abcam.cn/ps/products/80/ab80897/Images/ab80897-96143-anti-nox2-gp91phox-antibody-541-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-NOX2/gp91phox antibody [54.1] (ab80897)ICC/IF image of ab80897 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80897, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOX2/gp91phox antibody [54.1] (ab80897)](https://www.abcam.cn/ps/products/80/ab80897/Images/ab80897-96142-anti-nox2-gp91phox-antibody-541-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOX2/gp91phox antibody [54.1] (ab80897)IHC image of ab80897 staining in normal human skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab80897, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (20)
ab80897 被引用在 20 文献中.
- Jiang Z et al. NOX2 and NOX5 are increased in cardiac microvascular endothelium of deceased COVID-19 patients. Int J Cardiol 370:454-462 (2023). PubMed: 36332749
- Liu X et al. Resveratrol Improves Paclitaxel-Induced Cognitive Impairment in Mice by Activating SIRT1/PGC-1α Pathway to Regulate Neuronal State and Microglia Cell Polarization. Drug Des Devel Ther 17:1125-1138 (2023). PubMed: 37077409
- Dilly S et al. Targeting M2 Macrophages with a Novel NADPH Oxidase Inhibitor. Antioxidants (Basel) 12:N/A (2023). PubMed: 36830003
- de Jong JCBC et al. Evidence for sex-specific intramuscular changes associated to physical weakness in adults older than 75 years. Biol Sex Differ 14:45 (2023). PubMed: 37430322
- Maneta E et al. G-CSF induces CD15+ CD14+ cells from granulocytes early in the physiological environment of pregnancy and the cancer immunosuppressive microenvironment. Clin Transl Immunology 11:e1395 (2022). PubMed: 35602884