Key features and details
- Rabbit polyclonal to NOX1
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
参阅全部 NOX1 一抗
经测试应用适用于: ICC/IFmore details
Synthetic peptide corresponding to C terminal residues of human NOX1.
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, PBS
Concentration information loading...
纯度Immunogen affinity purified
纯化说明Purified by antigen specific affinity chromatography.
功能NOH-1S is a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes and other tissues. It participates in the regulation of cellular pH and is blocked by zinc. NOH-1L is a pyridine nucleotide-dependent oxidoreductase that generates superoxide and might conduct H(+) ions as part of its electron transport mechanism, whereas NOH-1S does not contain an electron transport chain.
组织特异性NOH-1L is detected in colon, uterus, prostate, and colon carcinoma, but not in peripheral blood leukocytes. NOH-1S is detected only in colon and colon carcinoma cells.
序列相似性Contains 1 FAD-binding FR-type domain.
Contains 1 ferric oxidoreductase domain.
细胞定位Cell projection > invadopodium membrane.
- Information by UniProt
- GP91 2 antibody
- Mitogenic oxidase (pyridine nucleotide dependent superoxide generating) antibody
- Mitogenic oxidase 1 antibody
ICC/IF image of ab55831 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55831, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab55831 被引用在 45 文献中.
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- Jiao P et al. The protective effect of doxofylline against lipopolysaccharides (LPS)-induced activation of NLRP3 inflammasome is mediated by SIRT1 in human pulmonary bronchial epithelial cells. Artif Cells Nanomed Biotechnol 48:687-694 (2020). PubMed: 32098511