Anti-NOX1抗体(ab121009)
Key features and details
- Goat polyclonal to NOX1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-NOX1抗体
参阅全部 NOX1 一抗 -
描述
山羊多克隆抗体to NOX1 -
宿主
Goat -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Rabbit, Guinea pig, Dog, Pig, Rhesus monkey, Gorilla
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免疫原
Synthetic peptide:
DKATDIVTGLKQK
with a Cysteine residue linker, corresponding to internal sequence amino acids 490-502 of Human NOX1 (NP_008983.2) or 441-453 of Human NOX1 (NP_039249.1). -
阳性对照
- WB: HeLa, CaCo-2, A549 and U937 cell lysates; IHC-P: Human kidney tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab121009 is purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab121009于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).
1 hour primary incubation is recommended for this product. |
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| IHC-P |
Use a concentration of 7.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
|
WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa). 1 hour primary incubation is recommended for this product. |
|
IHC-P
Use a concentration of 7.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
NOH-1S is a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes and other tissues. It participates in the regulation of cellular pH and is blocked by zinc. NOH-1L is a pyridine nucleotide-dependent oxidoreductase that generates superoxide and might conduct H(+) ions as part of its electron transport mechanism, whereas NOH-1S does not contain an electron transport chain. -
组织特异性
NOH-1L is detected in colon, uterus, prostate, and colon carcinoma, but not in peripheral blood leukocytes. NOH-1S is detected only in colon and colon carcinoma cells. -
序列相似性
Contains 1 FAD-binding FR-type domain.
Contains 1 ferric oxidoreductase domain. -
细胞定位
Cell projection > invadopodium membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 492016 Dog
- Entrez Gene: 27035 Human
- Entrez Gene: 701376 Rhesus monkey
- Omim: 300225 Human
- SwissProt: Q9Y5S8 Human
- Unigene: 592227 Human
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别名
- GP91 2 antibody
- Mitogenic oxidase (pyridine nucleotide dependent superoxide generating) antibody
- Mitogenic oxidase 1 antibody
see all
图片
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Western blot - Anti-NOX1 antibody (ab121009)All lanes : Anti-NOX1 antibody (ab121009) at 0.5 µg/ml
Lane 1 : CaCo-2 cell lysate
Lane 2 : CaCo-2 + peptide cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HeLa + peptide cell lysate
Lane 5 : A549 cell lysate
Lane 6 : A549 + peptide cell lysate
Lane 7 : U937 cell lysate
Lane 8 : U937 + peptide cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 65 kDaLysates in RIPA buffer. Detected by chemiluminescence.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOX1 antibody (ab121009)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling NOX1 with ab121009 at 7.5 µg/mL. Heat mediated antigen retrieval was performed using citrate buffer pH 6. An HRP-conjugated secondary antibody was used. Counterstained with hematoxylin.
Inset: Negative control showing staining of paraffin embedded human kidney, with no primary antibody.
数据表及文件
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Datasheet download
文献 (11)
ab121009 被引用在 11 文献中.
- Zheng R et al. Cordycepin from Cordyceps militaris ameliorates diabetic nephropathy via the miR-193b-5p/MCL-1 axis. Chin Med 18:134 (2023). PubMed: 37833817
- Wei X et al. Increased NOX1 and DUOX2 expression in the colonic mucosa of patients with chronic functional constipation. Medicine (Baltimore) 101:e30028 (2022). PubMed: 35960091
- Xu S et al. Naringenin alleviates myocardial ischemia/reperfusion injury by regulating the nuclear factor-erythroid factor 2-related factor 2 (Nrf2) /System xc-/ glutathione peroxidase 4 (GPX4) axis to inhibit ferroptosis. Bioengineered 12:10924-10934 (2021). PubMed: 34699317
- Jing Cao et al. Peroxidasin promotes diabetic vascular endothelial dysfunction induced by advanced glycation end products via NOX2/HOCl/Akt/eNOS pathway. Redox Biol 45:102031 (2021). PubMed: 34116361
- Wang Q et al. A natural product of acteoside ameliorate kidney injury in diabetes db/db mice and HK-2 cells via regulating NADPH/oxidase-TGF-β/Smad signaling pathway. Phytother Res 35:5227-5240 (2021). PubMed: 34236110
