JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB183024

重组Anti-Nova1抗体[EPR13847]

Anti-Nova1 antibody [EPR13847]

Be the first to review this product! Submit a review

|

(19 Publications)

Rabbit Recombinant Monoclonal NOVA1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human, Rat samples. Cited in 19 publications.

查看别名

RNA-binding protein Nova-1, Neuro-oncological ventral antigen 1, Onconeural ventral antigen 1, Paraneoplastic Ri antigen, Ventral neuron-specific protein 1, NOVA1

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nova1 antibody [EPR13847] (AB183024)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nova1 antibody [EPR13847] (AB183024)

Immunohistochemical analysis of paraffin-embedded human gliocytoma tissue labeling Nova1 using ab183024 at 1/500 dilution. HRP-conjugated Rabbit IgG was used as secondary (prediluted). Counterstain : Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nova1 antibody [EPR13847] (AB183024)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nova1 antibody [EPR13847] (AB183024)

Immunohistochemical analysis of paraffin-embedded Rat brain tissue labeling Nova1 using ab183024 at 1/500 dilution. A HRP conjugated Rabbit IgG was used as secondary (prediluted). Counterstain : Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Western blot - Anti-Nova1 antibody [EPR13847] (AB183024)
  • WB

Supplier Data

Western blot - Anti-Nova1 antibody [EPR13847] (AB183024)

All lanes:

Western blot - Anti-Nova1 antibody [EPR13847] (ab183024) at 1/5000 dilution

All lanes:

Human cerebellum lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab136636'>ab136636</a>) at 1/500 dilution

Predicted band size: 52 kDa

false

Western blot - Anti-Nova1 antibody [EPR13847] (AB183024)
  • WB

Supplier Data

Western blot - Anti-Nova1 antibody [EPR13847] (AB183024)

All lanes:

Western blot - Anti-Nova1 antibody [EPR13847] (ab183024) at 1/1000 dilution

Lane 1:

Mouse brain lysate at 10 µg

Lane 2:

PC12 lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 52 kDa

false

不同偶联物与剂型 (8)

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR13847

亚型

IgG

不含载体蛋白

No

反应种属

Mouse, Rat, Human

应用

IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "<p></p>" } } }
style
left-column

产品详情

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
style
left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Nova1 also known as Neuro-oncological ventral antigen 1 is a member of the Nova family of RNA-binding proteins. It has a molecular mass of approximately 53 kDa. Nova1 is predominantly expressed in the nervous system with high presence in neurons. The protein plays a role in the regulation of alternative splicing by binding to specific RNA sequences. This binding activity influences the splicing of various pre-mRNA transcripts highlighting the role of Nova1 in post-transcriptional gene regulation.
Biological function summary

Nova1 influences neuronal integrity by regulating RNA processing events. It binds to RNA motifs to ensure the accurate splicing needed for correct neuronal function. Nova1 functions as part of a multi-protein complex that includes spliceosomal components which help in recognizing splice sites in precursor RNA. The splicing activity of Nova1 affects the expression of numerous genes related to synaptic function and neurodevelopment which is critical for maintaining proper neuronal communication and plasticity.

Pathways

Nova1 participates in the neuronal alternative splicing pathway and influences synaptic signaling pathways. Its activity affects the pathways by regulating the splicing of various genes critical for these pathways. For example Nova1 is involved with proteins like Nova2 within these pathways. Their combined roles ensure that various proteins essential for synaptic function are produced in the correct isoforms which is necessary for synaptic health and plasticity.

Nova1 has been linked to neurodevelopmental disorders and certain cancers such as brain tumors. It plays a role in these diseases by dysregulating the alternative splicing of transcripts necessary for normal neuronal development and tumor suppression. Nova1 interacts with other proteins such as hnRNP proteins in the context of these disorders which further modulates the disease process. Understanding Nova1's roles helps in developing therapeutic strategies that target aberrant splicing events in these conditions.
style
left-column
style
left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

靶点信息

RNA-binding protein which regulates alternative splicing of pre-mRNAs in the brain and spinal cord in a sequence-specific manner (By similarity). Binds to 5'-YCAY-3' repeats, with a minimum of 2 to 3 repeats necessary for high-affinity binding. Mediates both exon inclusion and exclusion, depending upon the position of its binding site within the pre-mRNA (PubMed : 10811881). Binding to 5'-YCAY-3' clusters results in a local and asymmetric action to regulate spliceosome assembly. Binding to an exonic 5'-YCAY-3' cluster changes the protein complexes assembled on pre-mRNA, blocking U1 small nuclear ribonucleoprotein (snRNP) binding and inhibiting exon inclusion, whereas binding to an intronic 5'-YCAY-3' repeat enhances spliceosome assembly and favors exon inclusion (By similarity). Regulates the splicing of gamma-aminobutyric acid receptor subunit gamma-2 (GABRG2) and glycine receptor subunit alpha-2 (GLRA2) pre-mRNAs, among others (By similarity). Autoregulates its own splicing. Binds to its own exon 4 and directs its exclusion, thus leading to NOVA1 isoform 3 production (By similarity). May affect the splicing of many genes involved in vocal behavior (By similarity).
See full target information NOVA1
style
left-column

文献 (19)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:1542 PubMed39966351

2025

A humanized NOVA1 splicing factor alters mouse vocal communications.

Applications

Unspecified application

Species

Unspecified reactive species

Yoko Tajima,César D M Vargas,Keiichi Ito,Wei Wang,Ji-Dung Luo,Jiawei Xing,Nurdan Kuru,Luiz Carlos Machado,Adam Siepel,Thomas S Carroll,Erich D Jarvis,Robert B Darnell

Drug development research 85:e22185 PubMed38657094

2024

Upregulation of FGF9 and NOVA1 in cancer-associated fibroblasts promotes cell proliferation, invasion and migration of triple negative breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Zhang,Yang Liu,Jinsong Yu,Xi Lin

Proceedings of the National Academy of Sciences of the United States of America 121:e2314695121 PubMed38416679

2024

NOVA1 acts as an oncogenic RNA-binding protein to regulate cholesterol homeostasis in human glioblastoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yuhki Saito,Yanhong Yang,Misa Saito,Christopher Y Park,Kosuke Funato,Viviane Tabar,Robert B Darnell

Journal of translational medicine 21:879 PubMed38049848

2023

Altered splicing machinery in lung carcinoids unveils NOVA1, PRPF8 and SRSF10 as novel candidates to understand tumor biology and expand biomarker discovery.

Applications

Unspecified application

Species

Unspecified reactive species

Ricardo Blázquez-Encinas,Víctor García-Vioque,Teresa Caro-Cuenca,María Trinidad Moreno-Montilla,Federica Mangili,Emilia Alors-Pérez,Sebastian Ventura,Aura D Herrera-Martínez,Paula Moreno-Casado,Marco A Calzado,Ángel Salvatierra,María A Gálvez-Moreno,Lynnette Fernandez-Cuesta,Matthieu Foll,Raúl M Luque,Nicolas Alcala,Sergio Pedraza-Arevalo,Alejandro Ibáñez-Costa,Justo P Castaño

PloS one 18:e0289327 PubMed37531400

2023

Dynamics of TERT regulation via alternative splicing in stem cells and cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jeongjin J Kim,Mohammed E Sayed,Alexander Ahn,Aaron L Slusher,Jeffrey Y Ying,Andrew T Ludlow

Cell reports 42:112050 PubMed36716149

2023

NOVA1 acts on Impact to regulate hypothalamic function and translation in inhibitory neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Yoko Tajima,Keiichi Ito,Yuan Yuan,Mayu O Frank,Yuhki Saito,Robert B Darnell

BMC cancer 22:1091 PubMed36284263

2022

NOVA1 promotes NSCLC proliferation and invasion by activating Wnt/β-catenin signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Lianyue Qu,Yulong Tian,Fan Wang,Zixuan Li

Acta neuropathologica 144:413-435 PubMed35778567

2022

Aberrant NOVA1 function disrupts alternative splicing in early stages of amyotrophic lateral sclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Florian Krach,Emily C Wheeler,Martin Regensburger,Tom Boerstler,Holger Wend,Anthony Q Vu,Ruth Wang,Stephanie Reischl,Karsten Boldt,Ranjan Batra,Stefan Aigner,John Ravits,Juergen Winkler,Gene W Yeo,Beate Winner

Neural regeneration research 17:2530-2536 PubMed35535907

2022

NOVA1 promotes SMN2 exon 7 splicing by binding the UCAC motif and increases SMN protein expression.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Li Du,Jun-Jie Sun,Zhi-Heng Chen,Yi-Xiang Shao,Liu-Cheng Wu

Bioengineered 13:8982-8993 PubMed35348441

2022

MicroRNA miR-27a-3p accelerates cardiac hypertrophy by targeting neuro-oncological ventral antigen 1.

Applications

Unspecified application

Species

Unspecified reactive species

Dongyun Li,Mingzhi Shen,Xinxin Deng,Yongyi Bai
View all publications
style
left-column
style
left-column
style
right-column

替代产品

Primary Antibodies

AB97368

Anti-Nova1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Nova1 antibody (AB97368)

  • 4
  • 1
Primary Antibodies

AB183723

Anti-Nova1 antibody [EPR13848] - C-terminal

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nova1 antibody [EPR13848] - C-terminal (AB183723)

  • 5
  • 2

搭配使用产品

Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

  • 5
  • 104
Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 16
Primary Antibodies

AB96474

Anti-HuD antibody

Immunocytochemistry/ Immunofluorescence - Anti-HuD antibody (AB96474)

  • 0
  • 0
Primary Antibodies

AB198291

Anti-SRSF3 antibody [EPR16976]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-SRSF3 antibody [EPR16976] (AB198291)

  • 5
  • 6

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com