AB8387

Anti-Notch1 intracellular domain抗体

Anti-Notch1 intracellular domain antibody

Be the first to review this product! Submit a review

(5 Publications)

Rabbit Polyclonal Notch1 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 5 publications.

查看别名

TAN1, NOTCH1, Neurogenic locus notch homolog protein 1, Notch 1, hN1, Translocation-associated notch protein TAN-1

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Notch1 intracellular domain antibody (AB8387)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Notch1 intracellular domain antibody (AB8387)

ab8387 staining Notch1 in Human brain cerebellum tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde. Samples were incubated with primary antibody (1/500). Nuclear counter stain using hematoxylin (purple).

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

Notch1 antibody is directed against human NOTCH 1. No reaction is detected against NOTCH 2. No reactivity was observed against Mouse Notch. Other species have not been tested.

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/5000", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }

style

left-column

style

left-column

性能和储存信息

形式

Liquid

纯度

Whole antiserum

存储溶液

Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.42% Tripotassium orthophosphate

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The Notch1 intracellular domain often called NICD plays a significant role in cell signaling. NICD is approximately 120 kDa in mass and is primarily expressed in various tissues especially during development. It also shows expression in multiple cell types including T-cells in the immune system. The Notch1 protein undergoes proteolytic cleavage to release the NICD which then translocates to the nucleus to influence gene expression.

Biological function summary

The Notch1 intracellular domain acts as a transcriptional regulator within the Notch signaling pathway. It forms a complex with other proteins including the transcription factors RBPJ (Recombination Signal Binding Protein for Immunoglobulin Kappa J Region) and MAML (Mastermind-like) to modulate the transcription of target genes. This domain influences cell fate decisions differentiation and proliferation in many developmental processes. The Notch signaling pathway is often described as a communication system between adjacent cells conferring the ability to respond appropriately to developmental and environmental cues.

Pathways

The Notch1 intracellular domain has a critical function in the Notch signaling pathway and is inherently involved in the regulation of the canonical Wnt pathway. The NICD regulates interaction with the signaling protein β-catenin linking it to non-canonical Wnt signaling as well. Notch1 interacts closely with Delta-like and Jagged proteins which act as ligands to initiate the signaling cascade across cell membranes. The interactions among these components ensure precise control over cell differentiation and proliferation.

The Notch1 intracellular domain holds significance in the progression of cancers such as T-cell acute lymphoblastic leukemia (T-ALL) where aberrant Notch1 signaling leads to uncontrolled cell proliferation. In addition NICD has a connection to Alagille syndrome a genetic disorder affecting the liver heart and other organs which involves mutations in Jagged1. The links between NICD and these conditions highlight the importance of controlled Notch1 activity in maintaining tissue homeostasis and preventing pathological changes.

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

Functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Involved in the maturation of both CD4(+) and CD8(+) cells in the thymus. Important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, functions as a receptor for neuronal DNER and is involved in the differentiation of Bergmann glia. Represses neuronal and myogenic differentiation. May play an essential role in postimplantation development, probably in some aspect of cell specification and/or differentiation. May be involved in mesoderm development, somite formation and neurogenesis. May enhance HIF1A function by sequestering HIF1AN away from HIF1A. Required for the THBS4 function in regulating protective astrogenesis from the subventricular zone (SVZ) niche after injury. Involved in determination of left/right symmetry by modulating the balance between motile and immotile (sensory) cilia at the left-right organiser (LRO).

See full target information NOTCH1

style

left-column

文献 (5)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 12:1365705 PubMed38572484

2024

A maternal dorsoventral prepattern revealed by an asymmetric distribution of ventralizing molecules before fertilization in .

Applications

Unspecified application

Species

Unspecified reactive species

Aitana M Castro Colabianchi,Nicolás G González Pérez,Lucía F Franchini,Silvia L López

Cell 176:1379-1392.e14 PubMed30773315

2019

Lateral Inhibition in Cell Specification Mediated by Mechanical Signals Modulating TAZ Activity.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Xia,Daniel Gütl,Vanessa Zheden,Carl-Philipp Heisenberg

Development (Cambridge, England) 145: PubMed29866901

2018

Notch1 is asymmetrically distributed from the beginning of embryogenesis and controls the ventral center.

Applications

Unspecified application

Species

Unspecified reactive species

Aitana M Castro Colabianchi,Diego R Revinski,Paula I Encinas,María Verónica Baez,Renato J Monti,Mateo Rodríguez Abinal,Laurent Kodjabachian,Lucía F Franchini,Silvia L López

Clinical cancer research : an official journal of 17:5686-94 PubMed21771876

2011

Notch1 expression, which is related to p65 Status, is an independent predictor of prognosis in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Dake Chu,Yi Zhou,Zixi Zhang,Yuming Li,Jipeng Li,Jianyong Zheng,Hongwei Zhang,Qingchuan Zhao,Weizhong Wang,Rui'an Wang,Gang Ji

Cell cycle (Georgetown, Tex.) 6:418-29 PubMed17329972

2007

Notch, Myc and breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Argiris Efstratiadis,Matthias Szabolcs,Apostolos Klinakis

View all publications

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Notch1 intracellular domain抗体

Anti-Notch1 intracellular domain antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Notch1 intracellular domain antibody (AB8387)

关键信息

宿主种属

克隆

亚型

不含载体蛋白

反应种属

应用

免疫原

特异性